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Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680; Pusat Penelitian Sumberdaya Hayati & Bioteknologi (PPSHB)-LPPM IPB, Kampus IPB Darmaga, Institut Pertanian Bogor, Bogor 1668
Agriculture, Biological Sciences & Forestry

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Skrining dan Identifikasi Bakteri Laut Penghasil Enzim Selulase yang Berasosiasi dengan Spons Rika Indri Astuti; Yohanes Bernadino Putera Saju; Dina Aribah; Aris Tri Wahyudi
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.70

Abstract

Marine bacteria associated with sponges can be used as a source of new enzymes, especially cellulase which are potentially used for various industries. The aim of this study was to screen and identify the cellulolytic bacteria associated with sponges. A total of 38 isolates have been successfully isolated from sponges Aaptos sp., Euryspongia sp., and Haliclona sp. by a spread plate method. Amongst 38 isolates, 14 (37%) showed cellulolytic activities in carboxy methyl cellulose (CMC) medium with a cellulolytic index ranging from 0.31 to 1.63. The three highest cellulolytic index was exhibited by bacterial isolates coded Y.5.10, Y.5.11, and Y.40.6. Isolates Y.5.10, Y.5.11, and Y.40.6 showed cellulolytic activity of 0.0053 U/mL; 0.0083 U/mL; and 0.0124 U/mL, respectively, as were tested by spectrophotometry. The highest specific enzymatic activity was showed by isolate Y.40.6 with a value of 0.3391 U/mg. Based on 16S rRNA gene, isolate Y.5.10, Y.5.11, and Y.40.6 were highly similar to (similarity ≥ 99%) Bacillus sp., B. subtilis, and B. cereus, respectively. Keywords: cellulase, isolation, sponge-associated bacteria, 16S rRNA
Co-Authors Aris Tri Wahyudi Rika Indri Astuti Yohanes Bernadino Putera Saju