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All Journal Jurnal Ilmu Pertanian Indonesia Biogenesis: Jurnal Ilmiah Biologi
Dina Aribah
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680; Pusat Penelitian Sumberdaya Hayati & Bioteknologi (PPSHB)-LPPM IPB, Kampus IPB Darmaga, Institut Pertanian Bogor, Bogor 1668
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Pengujian Potensi Jus Tomat (Lycopersicum esculentum) Sebagai Terapi Herbal Pada Tikus Rattus norvegicus Hiperkolesterolemik Dina Aribah; Alif Rofiqotun Nurul Alimah; Anindya Nirmala Permata; Betty Lukiati
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 2 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i2.2512

Abstract

Hypercholesterolemia defines as the increasing of cholesterol serum that more than the normal limit. WHO (2011) on 2008 at Indonesian said that hypercholesterolemia prevalence of woman approach about 37,2% and for man about 32,8%. Tomato contain lycopene is one powerful antioxidant and can manage cholesterol metabolism with blocked the HMG-KoA enzyme reductase (enzyme for cholesterol synthesis in hepar). This study aims to determine the effect of tomato juice on the cholesterol levels in the blood of hypercholesterolemic rats and to find the effective dosis of tomato juice to decrease the cholesterol of hypercholesterolemic rats. The object of research covering 20 wistar rats (Rattus norvegicus) male 2-3 months old with 50-150g weight. The rats were divided into five groups and four replications: normal (K-), high-fat diet (K+), high-fat diet+tomato juice 200mg/100gBB (P1), high-fat diet+tomato juice 400mg/100gBB (P2), high-fat diet+tomato juice 800mg/100gBB (P3). Tomato juice was given orally for one month every day. At the end of the treatment of rats was dissected to take blood from the heart. Data were analyzed statistically with one-way ANAVA with α=0,05. The results showed that the tomato juice affects on the cholesterol levels of blood serum of hypercholesterolemic rats and the effective dose of tomato juice used in this study was 800mg/100gBB.
Skrining dan Identifikasi Bakteri Laut Penghasil Enzim Selulase yang Berasosiasi dengan Spons Rika Indri Astuti; Yohanes Bernadino Putera Saju; Dina Aribah; Aris Tri Wahyudi
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.70

Abstract

Marine bacteria associated with sponges can be used as a source of new enzymes, especially cellulase which are potentially used for various industries. The aim of this study was to screen and identify the cellulolytic bacteria associated with sponges. A total of 38 isolates have been successfully isolated from sponges Aaptos sp., Euryspongia sp., and Haliclona sp. by a spread plate method. Amongst 38 isolates, 14 (37%) showed cellulolytic activities in carboxy methyl cellulose (CMC) medium with a cellulolytic index ranging from 0.31 to 1.63. The three highest cellulolytic index was exhibited by bacterial isolates coded Y.5.10, Y.5.11, and Y.40.6. Isolates Y.5.10, Y.5.11, and Y.40.6 showed cellulolytic activity of 0.0053 U/mL; 0.0083 U/mL; and 0.0124 U/mL, respectively, as were tested by spectrophotometry. The highest specific enzymatic activity was showed by isolate Y.40.6 with a value of 0.3391 U/mg. Based on 16S rRNA gene, isolate Y.5.10, Y.5.11, and Y.40.6 were highly similar to (similarity ≥ 99%) Bacillus sp., B. subtilis, and B. cereus, respectively. Keywords: cellulase, isolation, sponge-associated bacteria, 16S rRNA
Co-Authors Alif Rofiqotun Nurul Alimah Anindya Nirmala Permata Aris Tri Wahyudi Betty Lukiati Rika Indri Astuti Yohanes Bernadino Putera Saju