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Rita Kurnia Apindiati
Institut Pertanian Bogor
Medicine & Pharmacology

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Identifikasi Polerovirus Penyebab Klorosis pada Cabai Asal Bali, Indonesia Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (790.801 KB) | DOI: 10.14692/jfi.11.2.43

Abstract

Based on the results of a survey conducted on chilipepper plants in Kertha Village, Payangan Subdistrict, Gianyar District, Bali Province, many chilipepper plants with chlorosis symptoms caused by the virus was observed. Infected plants showed yellowing of the lamina, but the veins remain green. Identification was focused on the Pepper vein yellows virus (PeVYV) through transmission using Aphis nasturtii, observation on the morphology of virus particles, total RNA detection by reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequence. The results showed that the disease can be transmitted from infected plant to healthy plant using A. nasturtii. Viral particle has hexagonal shape with the diameter ~30 nm. RT-PCR of total RNA from severe, moderate, and mild symptomatic infected plants, using specific primer sets for coat protein gene of PeVYV successfully amplified DNA with size of ~650 bp. Coat protein gene sequence of PeVYV isolates Bali, Indonesia showing different chlorosis intensity had the highest homology with PeVYV isolates from Taiwan 95.1–96.6%. Based on the data, PeVYV has been confirmed as the cause of chlorosis in chilipepper plants in Bali, Indonesia.
Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.953 KB) | DOI: 10.14692/jfi.11.4.137

Abstract

Pepper vein yellows virus (PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in E. coli strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.
Co-Authors Gede Suastika Kikin H Mutaqin