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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Pustakawan Indonesia Agrikultura BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Informatika Pertanian Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Pustakawan Indonesia JURNAL ECONOMINA

Kikin H Mutaqin

Institut Pertanian Bogor

Author-ID : 298800

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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RANCANG BANGUN SISTEM PAKAR PENANGGULANGAN PENYAKIT KEDELAI Astuti, Indah Puji; Hermadi, Irman; Buono, Agus; Mutaqin, Kikin H
Informatika Pertanian Vol 25, No 1 (2016): JUNI 2016
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Early detection and identification of soybean diseases is important to support better productivity of soybean. The demand for the availability of an expert on soybean disease is very high, especially for the beginners in the field of agriculture. However, the number and time allocation of the experts are not adequate to serve farmers located in different geographical areas. Therefore, an expert system is proposed as a solution to use as a diagnostic tool for soybean diseases just like a human expert. It will be even easier when the system is implemented into an Android-based application to be used anywhere and anytime. The objective of this study was to analyze and design an expert system for early identification of soybean diseases. This study was adopting the Expert System Development Life Cycle (ESDLC) approach. The stages were project initialization, knowledge engineering process, and implementation. The study was started with the project initialization phase that conducted in September 2014 and the completion of the implementationphase in August 2015. The results of research were in the form of document analysis and prototype system.

TEST OF BLOOD DISEASE BACTERIUM (BDB) TRANSMISSION BY POTENTIAL INSECT VECTORS Sahetapy, Betty; Maryana, Nina; Manuwoto, Syafrida; H. Mutaqin, Kikin; Latumahina, Fransina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12071-77

Blood disease bacterium (BDB) is one of the important diseases in banana and a major obstacle in developing and increasing banana production in Indonesia. The purpose of this study was to prove the ability of the Drosophilidae insect as a vector in transmitting BDB. The research was conducted at the Insect Biosystematics Laboratory and Plant Bacteriology Laboratory, Department of Plant Protection, Faculty of Agriculture, IPB University. Drosophilidae insects were taken from the field and then reared in laboratory by being fed with ripe bananas to obtain offspring that are free from diseases or pathogens. Imago of the Drosophilidae from rearing was fed by inoculum sources which was infected banana, then inoculated into healthy plants. The plants used were healthy and flowering, heliconia. The results showed that the Drosophilidae insects were able to transmit BDB to heliconia plants that showed symptoms, brownish flower colors and falling flower crowns. Detection of BDB isolated from flower parts and the inside parts of the insects used in transmission test using the PCR method showed positive results.

KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Mutaqin, Kikin Hamzah; ., Giyanto; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.

Efektivitas Formulasi Spora Bacillus subtilis B12 sebagai Agen Pengendali Hayati Penyakit Hawar Daun Bakteri pada Tanaman Padi Wartono, Wartono; Giyanto, Giyanto; Mutaqin, Kikin H.
Jurnal Penelitian Pertanian Tanaman Pangan Vol 34, No 1 (2015): April 2015
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Bacterial leaf blight control in rice (Oryza sativa Lin.) using bactericide is prohibitive, due to its high cost and its negative effect on the environment. Biocontrol when avaible, therefore is the best alternative solution. Bacillus subtilis is a perspective of biocontrol agent to control several plant diseases, because of its ability to produce antimicrobial and produce plant growth promoting substances. This research was aimed to examine the effectiveness of B. subtilis spore formulation by way of seed treatments and foliar sprays, using different frequencies and concentrations, to control bacterial leaf blight disease (BLB) on rice, caused by Xanthomonas oryzae pv. oryzae, and to evaluate its function as plant growth promoter. The experiments were conducted at greenhouse and in the field using factorial design. At the greenhouse experiment, seed treatment and foliar spray, using concentration of 2% produced better result in controlling BLB, and better result on promoting rice plant growth. In the field experiment, application at 2 week interval showed better effect on suppressing the the disease and on increasing yield. Applications of the formulation of B. subtilis B12 spore reduced BLB disease by 21% and potentially increased yield up to 50%.

Taksasi Kehilangan Hasil oleh Penyakit Kerdil pada Kentang di Jawa Tengah Sofranita Syifa Fitriyati; Kikin Hamzah Mutaqin; Tri Asmira Damayanti
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Potato is one of economically important crops in Indonesia. However, its production fluctuates since there are some factors affecting the yield. Recently, we found dwarf disease at potato central production area in Wonosobo and Banjarnegara Regencies in Central Java. So far, there is no information related to the yield loss caused by the dwarf disease on potato. Therefore, the research aimed to estimate the yield loss caused by the dwarf disease on potato in Central Java. The yield loss estimation was determined on some potato cultivations with different disease severities. The disease intensity was observed for five times with interval two weeks on six plots. The quantity and quality of potato yields, and selling price was calculated at the end of the observation and the correlation between the severity of disease and AUDPC (Area under disease progress curve) with yield loss was determined by regression and correlation analysis. The dwarf disease reduced plant height, individual and total tuber weight without affecting the number of tuber yield. The different of disease intensities in some area affected to the percentage of yield loss. The yield loss caused by dwarf diseases on individual potato plants up to 64.42%. Yield loss estimation caused by the dwarf disease in fields with different level of disease intensities ranged from 10.35-26.43%. Regression analysis between disease severities and yield losses showed the R square value at the level of 88.6% and a correlation at the level of 94.14%. These results indicated that the correlation between the severity of dwarf disease and yield loss is high. Keywords: disease severity, potato yield, selling price, tuber quality

Sapu pada Kacang Hias (Arachis pintoi): Penyakit Baru yang Berasosiasi dengan Fitoplasma Budiyarto .; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pinto peanut (Arachis pintoi) witches’ broom is a new disease found in Bogor at high incidence and has not yet been reported elsewhere. Symptom type of the disease is very similar to that of peanut (A. hypogea) witches’ broom, which has been well known to be associated with phytoplasma. Both A. pintoi and A. hypogea witches’ broom causal agent are transmissible to both healthy A. pintoi or A. hypogea, with grafting or vector transmission using peanut witches’ broom phytoplasma specific vector, Orosius argentatus. All combination of transmission means resulted in an identical type of witches’ broom symptom. Further confirmation using polymerase chain reaction detection and identification of phytoplasma employing universal primer pair for phytoplasmas (P1/P7), showed that all of the diseased plants, either from field or transmitted plants is associated with phytoplasma. The A. pintoi witches’ broom phytoplasma is likely identical to that of A. hypogea.Key words: Arachis pintoi, Orosius argentatus, phytoplasma, witches’ broom

Pemanfaatan Mulsa Jerami dan Plant Growth Promoting Rhizobacteria untuk Menekan Penyakit Pustul Bakteri pada Tanaman Kedelai Tita Widjayanti; Abdjad Asih Nawangsih; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial pustule caused by Xanthomonas axonopodis pv. glycines is one of important bacterial diseases of soybean in Indonesia. The development of alternative control of the disease is required to support integrated diseases management for sustainable agriculture. This research was conducted to observe the effect of straw mulch and plant growth promoting Rhizobacteria (PGPR) on the incidence of bacterial pustule disease on two soybean varieties in the farmer field at Cigombong, Sukabumi, West Java. The result of this research showed that all of the factors as combinations did not significantly affect the value of total area under disease progress curve (AUDPC) of bacterial pustule. Factor that significantly affected the values of AUDPC was soybean varieties. Gepak Kuning variety has lowest AUDPC value compared to Anjasmoro variety. In addition, combination of Gepak Kuning variety and straw mulch showed significantly different effect on AUDPC value. Therefore, this treatment combination should be recommended for the farmers.Key word: PGPR, soybean variety, straw mulch, Xanthomonas axonopodis pv. glycines

Penularan Fitoplasma Sapu pada Tanaman Kacang Tanah oleh Serangga Vektor Orosius argentatus dan Deteksi Molekuler dengan Teknik PCR Tatit Sastrini; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Witches’ broom disease caused by phytoplasma is a very serious disease on peanut (Arachis hypogaea) which may potentially lead to high yield loss. Insects are the most important agents of phytoplasma transmission in the field. The objective of this research was to examine the potential role of leafhoppers species as insect vector of phytoplasma and to determine their transmission characteristic. Two species of leafhopper i.e. O. argentatus and Empoasca sp. (both belong to Hemiptera: Cicadellidae) were chosen for this study. The methodology involved were transmission study of phytoplasma by O. argentatus and Empoasca sp., and molecular detection of phytoplasma by PCR technique to confirm the association of pathogen, insect vector and symptomatic plants. The result showed that specific symptom was observed when using O. argentatus in the transmission study with number of insect as low as 1 insect per plant, whereas Empoasca sp. was not able to transmit the disease. Incubation period of phytoplasma in the host plant was affected by the number of insect, i.e. the more insect vector the shortest incubation period. The phytoplasma was successfully detected using P1/P7 primer in symptomatic plants as well as in the insect vector.Key words: Empoasca sp., leafhoppers, polymerase chain reaction

Identifikasi Polerovirus Penyebab Klorosis pada Cabai Asal Bali, Indonesia Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Based on the results of a survey conducted on chilipepper plants in Kertha Village, Payangan Subdistrict, Gianyar District, Bali Province, many chilipepper plants with chlorosis symptoms caused by the virus was observed. Infected plants showed yellowing of the lamina, but the veins remain green. Identification was focused on the Pepper vein yellows virus (PeVYV) through transmission using Aphis nasturtii, observation on the morphology of virus particles, total RNA detection by reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequence. The results showed that the disease can be transmitted from infected plant to healthy plant using A. nasturtii. Viral particle has hexagonal shape with the diameter ~30 nm. RT-PCR of total RNA from severe, moderate, and mild symptomatic infected plants, using specific primer sets for coat protein gene of PeVYV successfully amplified DNA with size of ~650 bp. Coat protein gene sequence of PeVYV isolates Bali, Indonesia showing different chlorosis intensity had the highest homology with PeVYV isolates from Taiwan 95.1–96.6%. Based on the data, PeVYV has been confirmed as the cause of chlorosis in chilipepper plants in Bali, Indonesia.

Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pepper vein yellows virus (PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in E. coli strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.

Co-Authors . Giyanto Abdjad Asih Nawangsih Abdul Munif Ade Syahputra Af’idzatuttama Agus Buono Agus Eko Prasetyo Agus Purwito Ali Husni ALI NURMANSYAH Ana Feronika Cindra Irawati Ani Widarti Anna Feronika Cindra Irawati Ariny Prasetya Asmar Hasan Astuti, Indah Puji Asysyuura, Asysyuura Auliya Selamet Bonny Poernomo Wahyu Soekarno Budi Tjahjono BUDI TJAHJONO Budiyarto . Christian, Michael Dede Maryana Diana Putri Dono Wahyuno Efi Toding Tondok Eny Widajati Fitri Kemala Sandra Fitrianingrum Kurniawati, Fitrianingrum Fransina Latumahina Gede Suastika Giyanto Giyanto . Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Hagia Sophia Khairani Hamidi, Ilmi Hidayat, Sri Indah Puji Astuti Indah Puji Astuti Indriati Husain Irman Hermadi Irsan Nuhantoro Isti Wulandari John Thomas Jubaedah, Dedah Kresnamurti Kurniasih Kristi, Agusti Kusumah, Yayi Munara Laksono Trisnantoro Leiwakabessy, Christoffol Maggy Tenawidjaja Suhartono Maggy Tenawidjaja Suhartono MEITY SURADJI SINAGA Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Miftakhurohmah Muhammad Firdaus Oktafiyanto Muhammad Rizal nFN Sulastri nFN Widodo Nurfadillah PURNAMA HIDAYAT Rita Kurnia Apindiati Rizky G S Purnama RUSMILAH SUSENO Sahetapy, Betty Sari Nurulita Siska Irhamnawati Pulogu Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sri Kurniawati Sudir Sudir, Sudir Sulaeman Sulaeman Sulastri Sulastri Supramana Supriadi Suryo Wiyono Syafrida Manuwoto SYAFRIDA MANUWOTO Tatit Sastrini Tita Widjayanti TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Wahyudin, Denih Wartono, Wartono Widi Amaria Widodo Widodo Widodo Widodo Yadi Janwari Yayu Siti Nurhasanah Yudi Sastro Yudi Sastro Yuliana Susanti Yuliani, Fitria

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