Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Fitopatologi Indonesia
Giyanto Giyanto
Institut Pertanian Bogor, Bogor 16680
Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Keefektifan Perlakuan Fisik dan Minyak Atsiri untuk Mengeliminasi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Siti Tri Wahyuni; Ali Nurmansyah; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.454 KB) | DOI: 10.14692/jfi.13.4.136

Abstract

Clavibacter michiganensis subsp. michiganensis is the causal agent of bacterial canker disease of tomato. The bacteria is seed borne and may cause yield loss up to 70%. Seed treatment is an alternative method for controlling bacterial canker. The objective of the research was to study the effectiveness of physical and essential oil treatment for elimination of C. michiganensis subsp. michiganensis from tomato seed. Research was conducted in 4 separate experiments, i.e. (1) screening essential oils to control C. michiganensis subsp. Michiganensis; (2) to determine the treatment window of physical and essential oil treatment; (3) to determine the physical and essential oil treatment on tomato seed infested by C. michiganensis subsp. Michiganensis; and (4) to determine the effectiveness of treatment combination of physical and essential oil. The results showed that betel oil at concentration of 8% had the greatest inhibitory level (approximately 27.33 mm). Hot water treatment (53 °C) for 25 minutes reduced 90.94% of C. michiganensis subsp. michiganensis population; whereas dry heat treatment (60 °C, 24 hours) and betel oil treatment at 0.25% concentration reduced C. michiganensis subsp. michiganensis population (85.13 and 99.82% respectively). The combination of betel oil (0.5%), hot water (55 °C), and dry heat treatments (60 °C) was the most effective control method, because it reduced 99.99% of C. michiganensis subsp. michiganensis population and maintained the germination level of seed up to 100%.  This combination might be recommended for seed treatment to eliminate C. michiganensis subsp. michiganensis.
Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp. Ariny Prasetya; Kikin Hamzah Mutaqin; Meity Suradji Sinaga; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.327 KB) | DOI: 10.14692/jfi.13.4.145

Abstract

Cactus species (Opuntia sp.) is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done.  This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma.  BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma). This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.   
Co-Authors ALI NURMANSYAH Ariny Prasetya Kikin H Mutaqin Meity Suradji Sinaga Siti Tri Wahyuni