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All Journal Jurnal Fitopatologi Indonesia Buletin Penelitian Tanaman Rempah dan Obat Jurnal Ilmu dan Teknologi Kayu Tropis Buletin Penelitian Tanaman Rempah dan Obat
Arief Heru Prianto
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Medicine & Pharmacology

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Evaluation of Plant-based Pesticide containing Neem Extract (Azadirachta sp.) to Control Anthracnose Growth in Chili Fruits Yashanti Berlinda Paradisa; Wahyuni; Enung Sri Mulyaningsih; Ambar Yuswi Perdani; Arief Heru Prianto
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.112-122

Abstract

Cabai merupakan salah satu produk hortikultura yang banyak dikonsumsi di Indonesia. Antraknosa merupakan salah satu penyakit utama dalam budi daya cabai. Kehilangan hasil akibat antraknosa mencapai 35%. Penelitian ini bertujuan untuk menentukan efektivitas pestisida nabati dengan bahan utama ekstrak mimba dalam mengendalikan antraknosa pada cabai. Penelitian ini dilakukan di Pusat Penelitian Bioteknologi, LIPI. Pengujian in vitro dilakukan menggunakan rancangan acak kelompok faktorial yang diulang 4 kali dengan faktor pertama ialah cendawan Colletotrichum acutatum dan Colletotrichum gloeosporioides; faktor kedua ialah pestisida nabati dengan bahan aktif mimba (Agr I dan Agr II); serta faktor ketiga berupa 6 taraf perlakuan konsentrasi pestisida (0%, 0.5%, 1%, 2%. 3%, 4%, dan 5%). Pada pengujian in vivo dilakukan menggunakan rancangan acak kelompok faktorial dengan 4 ulangan. Faktor pertama ialah cabai besar dan cabai keriting; faktor kedua ialah C. acutatum dan C. gloeosporioides; dan faktor ketiga ialah 4 taraf konsentrasi pestisida Agr I (0%, 5%, 10, dan 15%). Berdasarkan hasil pengujian diketahui bahwa pestisida nabati Agr I dan Agr II dapat menghambat pertumbuhan cendawan secara in vitro dan Agr I lebih potensial untuk mengendalikan Colletotrichum spp. Namun pestisida nabati Agr I tidak mampu mengendalikan patogen yang telah berada di dalam jaringan tanaman.
The Synergy Effect of Azadirachtin and Minor Components of Neem Seed Oil on Antifeedant Activity of Spodoptera litura Arief Heru Prianto; NFN Budiawan; Yoki Yulizar; Partomuan Simanjuntak
Buletin Penelitian Tanaman Rempah dan Obat Vol 30, No 1 (2019): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v30n1.2019.27-34

Abstract

Azadirachtin in neem oil (Azadirachta indica A.Juss) might have potential synergism effect with a minor compound in the neem oil as an antifeedant. This study aimed to determine the content of minor components that have the most significant contribution to the effect of synergy with the main component (azadirachtin) in antifeedant activity against Spodoptera litura. Neem oil was fractionated with n-hexane, ethyl acetate, and water. The antifeedant activity of three neem oil fractions was evaluated using the 4th instar of S. litura larvae. The bioassay was conducted using the choice leaf disc test with 2.5; 5; 10; and 20 % (v/v) concentrations of each fraction. The concentration of the fractions showed equal azadirachtin content, hence the antifeedant activity represented the effect of the minor component in the fraction. The best activity was shown by water fraction (84-100 %). Probit analysis of the antifeedant activity of all fractions showed that water fraction has the best effective inhibitory (EI50) at 1.0 %.  The minor component in the water fraction was dominated by phenol compounds (48.5 %) which were suspected to synergize with the main component (azadirachtin) in increasing the antifeedant activity on S. litura. These results indicated the phenol compound in this fraction has the role in increasing azadirachtin antifeedant activity against S. litura.
The Synergy Effect of Azadirachtin and Minor Components of Neem Seed Oil on Antifeedant Activity of Spodoptera litura Arief Heru Prianto; NFN Budiawan; Yoki Yulizar; Partomuan Simanjuntak
Buletin Penelitian Tanaman Rempah dan Obat Vol 30, No 1 (2019): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v30n1.2019.27-34

Abstract

Azadirachtin in neem oil (Azadirachta indica A.Juss) might have potential synergism effect with a minor compound in the neem oil as an antifeedant. This study aimed to determine the content of minor components that have the most significant contribution to the effect of synergy with the main component (azadirachtin) in antifeedant activity against Spodoptera litura. Neem oil was fractionated with n-hexane, ethyl acetate, and water. The antifeedant activity of three neem oil fractions was evaluated using the 4th instar of S. litura larvae. The bioassay was conducted using the choice leaf disc test with 2.5; 5; 10; and 20 % (v/v) concentrations of each fraction. The concentration of the fractions showed equal azadirachtin content, hence the antifeedant activity represented the effect of the minor component in the fraction. The best activity was shown by water fraction (84-100 %). Probit analysis of the antifeedant activity of all fractions showed that water fraction has the best effective inhibitory (EI50) at 1.0 %.  The minor component in the water fraction was dominated by phenol compounds (48.5 %) which were suspected to synergize with the main component (azadirachtin) in increasing the antifeedant activity on S. litura. These results indicated the phenol compound in this fraction has the role in increasing azadirachtin antifeedant activity against S. litura.
Daya Patogenitas Cendawan Cunninghamella sp. terhadap Rayap Tanah Coptotermes sp. Pathogenic Ability of Cunninghamella sp. against Subterranean Termites Coptotermes sp Titik Kartika; Suciatmih Suciatmih; Didi Tarmadi; Ikhsan Guswenrivo; Arief Heru Prianto; Sulaeman Yusuf
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 4, No 1 (2006): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.355 KB) | DOI: 10.51850/jitkt.v4i1.285

Abstract

Termites are the major agent in wood biodeterioration, particularly in building materials, thus its existence in wood become trouble. Chemical insecticides are widely used to prevent the lost from termites attack. The use of chemical insecticides, however, do not recomended as it may cause risk to human health, environment, and may also harm to the non-target organisms and lead to the development of pest resistance. For that reason, these situations encourage many scientists to develop and evaluate various prospective biological control of termites. One of them is utilization of entomopathogenic fungi to suppress termite population.The object of this research is learning pathogenic ability of Cunninghamella sp. (habitually found around termites nests) in termite’s mortality. The methods of this research are fungal isolation and then pathogenic test to termites (termite’s infection). The ways of termite infection are contact and digestion. The best method is based on termite’s mortality rate after infection.The results show that Cunninghamella sp. causes termites mortality significantly than control. The higher mortality rate of termites is shown by contact infection method than digestion infection method. It might indicate that contact infection method more effective to suppress termite’s population
Co-Authors Ambar Yuswi Perdani Didi Tarmadi Enung Sri Mulyaningsih Ikhsan Guswenrivo NFN Budiawan Partomuan Simanjuntak Suciatmih Suciatmih Sulaeman Yusuf Titik Kartika Wahyuni Yashanti Berlinda Paradisa Yoki Yulizar