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JAMUR ENTOMOPATOGEN DAN AKTIVITAS ENZIM EKSTRASELULERNYA Suciatmih Suciatmih; Titik Kartika; Sulaeman Yusuf
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1818

Isolation of entomopathogenic fungi and their extracellular enzyme activity from rhizosphere soil were carried out. Soil samples were collected from rhizosphere under Arecaceae plant collection and a shrub in Bogor Botanic Garden, West Java; and another samples were gathered from forest floor on peat land in Sebangau National Park, Central Kalimantan. Insect bait method was used to catch fungi from soil samples by using Coptotermes sp. termites and Xystrocera festiva larva in the laboratory. A total of 38 fungal isolates belonging to 18 species and 12 genera were successfully isolated and identified. Isolated fungi were dominated by Deuteromycotina; and Fusarium is the most common (5 species). Peat and Pinanga coronata rhizospheres; and termites bait gave the highest fungal diversity of 9 species respectively. All fungal isolates did not indicate chitinase activity, but 60.53 %, 10.53 % and 13.16 % had both of lipase and protease; lipase and protease activities, respectively. Only 15.79 % fungal isolates were negatively in lipase or protease activities.

Daya Patogenitas Cendawan Cunninghamella sp. terhadap Rayap Tanah Coptotermes sp. Pathogenic Ability of Cunninghamella sp. against Subterranean Termites Coptotermes sp Titik Kartika; Suciatmih Suciatmih; Didi Tarmadi; Ikhsan Guswenrivo; Arief Heru Prianto; Sulaeman Yusuf
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 4, No 1 (2006): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Termites are the major agent in wood biodeterioration, particularly in building materials, thus its existence in wood become trouble. Chemical insecticides are widely used to prevent the lost from termites attack. The use of chemical insecticides, however, do not recomended as it may cause risk to human health, environment, and may also harm to the non-target organisms and lead to the development of pest resistance. For that reason, these situations encourage many scientists to develop and evaluate various prospective biological control of termites. One of them is utilization of entomopathogenic fungi to suppress termite population.The object of this research is learning pathogenic ability of Cunninghamella sp. (habitually found around termites nests) in termite’s mortality. The methods of this research are fungal isolation and then pathogenic test to termites (termite’s infection). The ways of termite infection are contact and digestion. The best method is based on termite’s mortality rate after infection.The results show that Cunninghamella sp. causes termites mortality significantly than control. The higher mortality rate of termites is shown by contact infection method than digestion infection method. It might indicate that contact infection method more effective to suppress termite’s population

Pemanfaatan Ekstrak Enzim Jamur Humicola sp. Sebagai Biotermitisida Utilization of Humicola sp. Enzyme Extract as Biotermiticide Ikhsan Guswenrivo; Titik Kartika; Didi Tarmadi; Sulaeman Yusuf
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 6, No 1 (2008): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Fungal spore can be used for biotermiticide, since it has high pathogen properties and environmentally safe. However, the utilization of enzyme extract of fungi can be developed as an alternative way to minimize the impact caused by the spore when they are used as biotermiticide. This research was done to observe the ability of enzyme extract from Humicola sp. The fungus was inoculated in sorghum substrate and grown in it by adding either colloidal chitin or without colloidal chitin to the media as variation of the medium composition. Extraction was done after 3, 5, and 7 days of incubation time. Extract was done by using cold sterilized water (40C). Then the extract kept it at low temperature (40C). Bioassay of extract enzyme was done by spraying and baiting method. Result of the bioassay showed, Humicola sp enzyme extract was grown from medium adding with colloidal chitin and incubation for 7 days give highest mortality of termite increase to 100% after 14th day observation.

Pengaruh Ekstrak Bintaro (Carbera odollam Gaertn) dan Kecubung (Brugmansia candida Pers) terhadap Rayap Tanah Coptotermes sp Influence of Bintaro (Carbera odollam Gaertn) and Kecubung (Brugmansia candida Pers) Extract against Subterranean Termite Coptotermes sp Didi Tarmadi; Arief H Prianto; Ikhsan Guswenrivo; Titik Kartika; Sulaeman Yusuf
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 5, No 1 (2007): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Subterranean termite especially Coptotermes sp has been spread widely and has caused a tremendous economical loss. Various chemical compounds have been used to overcome termite attack. However, utilization of chemical could endanger the environment, thus it is important to find another compound which can be used as an environmental friendly wood preservatives. One of the alternatives is to extract natural compound which has an anti-insect peculiarity. Fruits, leaves and barks of Bintaro and Kecubung, especially their leaves and flowers have been widely known as traditional medicine. This paper explains the effects of the extracts of Bintaro (leaves and bark) and Kecubung (leaves) on subterranean termite of Coptotermes sp.Leaves and bark powder of Bintaro and leaves powder of Kecubung were extracted with n-hexane, ethyl acetate, acetone, and methanol. Paper disc which has been dropped by the extract solution was used as bait to subterranean termite of Coptotermes sp. The observation of termite mortality was calculated for every two days during 10 days of observation. The result shows that Bintaro leaves extracted with methanol caused 100% termite mortality on last observation. Whereas on Bintaro bark extracted with n-hexane and acetone caused 100% termite mortality on eighth day of the treatment. Kecubung leaves extracted with n-hexane and ethyl acetate caused 100% termite mortality on last observation

Keefektifan Beberapa Spesies Cendawan Entomopatogen untuk Mengendalikan Rayap Tanah Coptotermes gestroi WASMANN (Isoptera: Rhinotermitidae) dengan Metode Kontak dan Umpan Effectiveness of Some Entomopathogenic Fungi Species as Bio-control Agent to Subterranean Termite Coptotermes gestroi WASMANN (Isoptera: Rhinotermitidae) Using Contact and Baiting Methods Desyanti Desyanti; Yusuf Sudo Hadi; Sulaeman Yusuf; Teguh Santoso
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 5, No 2 (2007): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Species of entomopathogenic fungi, i.e. Metarhizium anisopliae (Metsch.) Sorokin, Metarhizium brunneum Petch, Beauveria bassiana (Bals.) Vuillemin, Fusarium oxysporum Link and Aspergillus flavus Link were tested their effectiveness as bio-control against to subterranean termite Coptotermes gestroi Wasmann. Pure culture of each fungal species was stored in 40C. Before used, those fungi were recultured in Sabouraud Dextrose Agar with Yeast Extract (SDAY) media and incubated in 240C and RH 95% for 3 weeks. The series of conidial density has been used (0, 107, 5x106, 106, 5x105 and 105 conidia/ml) for pathogenicity test. The LC95 of M. brunneum was used for contact and baiting methods and the bioassay was repeated four times. The data was analyzed using ANOVA and with DNMRT. The lethal concentration (LC) and lethal time (LT) were calculated using probit analysis. The results revealed that increase of conidial density of each fungi species caused more mortality of C. gestroi. All fungi species could cause mortality of C. gestroi more than 80% even with the density of 5x106 conidia/ml, however M. brunneum could causing mortality more than 80% at density of conidia as low as 5x105 conidia/ml. Based on the probit analysis, the value of LC50 and LT50 with contact method were calculated at 1.8 x 105 conidia/ml and 2.01 (1.52 ~ 2.40) days respectively.

Ketahanan Kayu terhadap Serangan Rayap Tanah dengan Perlakuan Garam Metal The Subterranean Termites Resistance of Metal-Salt Treated Wood Sulaeman Yusuf; Takeshi Furuno
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 3, No 1 (2005): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Treatment of wood with a boron compound (inorganic substance) having both fire and biological resistance, dimensional stability and other features has been studied. In this experiment, borax and metal-salt treated wood were studied to evaluate the termite resistance. The water-saturated wood specimens were first impregnated by a saturated borax solution and then diffuse-penetrated with Zn and Cu. The second specimens were impregnated with colloidal silica and evaluated the effect of wood treatment by subterranean termites.The results indicated that colloidal-silica and methaborate-salt showed generally good termite resistance with negligible mass loss of specimens

Pengembangan Formula Bahan Infeksi Cendawan sebagai Alternatif Biokontrol Rayap Tanah Coptotermes sp. Development of Infection Material Formula for Fungi as Bio-Control Alternative to Subterranean Termites Coptotermes sp Titik Kartika; Sulaeman Yusuf; Didi Tarmadi; Arief Heru Prasetyo; Ikhsan Guswenrivo
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 5, No 2 (2007): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Utilization of fungal entomopathogen as biological control agent has been developed in several country, but not in Indonesia. Therefore, the use of biological control agent to control termite need to be done in order that diminish chemical insecticide hazard. In this research, the ability of fungal entomopathogen (Hyphomycetes) identified as Metarhizium anisopliae, Beauveria sp. and Humicola sp. to infect Subterranean termite will be evaluated. The fungi were isolated from Indonesia. The research methods are (i) to sporulate fungi in rice culture media; (ii) to formulate fungal entomopathogen being infection materials; (iii) bioassay of fungal entomopathogen against Subterranean termite by contact method. The result of bioassay show that the three fungi (Humicola, M. anisopliae and Beauveria) are able to kill termite in 14 days of observation day. Primarily, M. anisopliae has generate termite’s mortality almost similar to Humicola sp. i.e higher than 60 %, in other side Beauveria just affect termite’s mortality lower than 60 %.

Insecticide Activity of Cerbera manghas Fruit Exstract to Sitophilus oryzae (Coleoptera: Curculionidae) Ikhsan Guswenrivo; Didi Tarmadi; Sulaeman Yusuf
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 1 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

It was informed that the fruit extract of Cerbera manghas has antitermic and bio-larvicidal activities. The objective of this research is to study the effect of Cebera manghas fruit extracts on the mortality of Sitophilus oryzae (Coleoptera: Curculionidae). Dried sample of C. manghas fruit was milled and then extracted with maceration method using methanol. Crude extract was then dried and fractioned into 2 fractions i.e. n-Hexane and ethyl acetate. Three concentration of extracts (i.e. 3, 5, and 7.5%) were applied for bioassay test in the difference baited time (i.e. 30 min, 1 h, and 3 h). The results indicated that n-hexane fraction of C. manghas fruit extract was the most active fraction and it was indicated by the highest mortality of S. oryzae at all concentration. The longer the baited time resulted the higher the mortality of S. oryzae.Key words: bio-assay, Cerbera manghas, extractive, insecticide activity, Sitophilus oryzae

Pengembangan Teknologi Pengendalian Serangga Hama Gudang Menggunakan Pestisida Alami Berbasis Nimba {Azadirachta indica. A.Juss) Development of Stored Product Pest Control Technology Using Biopesticide Based on Neem (Azadirachta indica. A. Juss) Sulaeman Yusuf; Khoirul Himmi Setiawan; Didi Tarmadi; Deni Zulfiana; Maya Ismayati; Atik Setyowati
JURNAL PANGAN Vol. 21 No. 3 (2012): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v21i3.153

Selama dalam masa penyimpanan, komoditi hasil panen dapat mengalami kerusakan dan susut bobot (losses) yang disebabkan oleh serangan hama. Sitophilus oryzae adalah salah satu penyebab kerugian dan kerusakan terbesar karena memiliki kemampuan cepat berkembang biak akibat suplai makanan yang melimpah, mampu berpindah bersama-sama dengan komoditas hasil panen, serta mempunyai daya adaptasi pada kondisi kering. Pengendalian secara kimiawi tidak menjadi solusi, karena masih menggunakan bahan-bahan pestisida berbahaya seperti Carbamat, Piretroid, PH3 dan Metil Bromida,yang memiliki efek karsinogenik yang memicu kanker, akibat efek residual yang tertinggal dalam komoditas pangan. Berdasarkan hal tersebut, penelitian ini menawarkan teknologi pengendalian hama gudang penyimpanan hasil panen, menggunakan pestisida alami berbasis nimba, Azadirachta indica yang efektif, aman sekaligus ramah lingkungan. Hasil bioassay menunjukkan respon yang sangat baik, dimana mortalitas 100 persen S. orizae dicapai pada konsentrasi 10 ppm produk formulasi. Uji toksisitas akut oral dan dermal juga menunjukkan produk formulasi termasuk kategori bahan tidak berbahaya (WHO, 2003).During storage, stored product commodities could be damaged and lose weight caused by pest attack. Sitophilus oryzae is one of the most dangerous stored pests because of their fast reproductive abilities due to abundant supply of foods; their moving abilities with stored commodities; and their adaptability to extremely drying condition.Chemical treatments are not solving the problems because they still contain some toxical substances like carbamate, pyretroid, methyl boric, that would lead to residual contain remains on food commodity and possess carsinogenic effect Based on that background, this research proposes new stored product pest control technology using effective and environmentally friendly biopesticide based on neem (Azadirachta indica A. Juss). Bioassay results against S. oryzae show that 100 percent mortality is achieved at 10 ppm biopesticide formulation. Both acute oral and dermal toxicity tests also show that biopesticide formulation is classified as not harmful substances (WHO, 2003).

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