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Resistance of Several Hibiscus cannabinus genotypes Against Meloidogyne incognita Parnidi Parnidi; Lita Soetopo; Damanhuri Damanhuri; Marjani Marjani
Jurnal Fitopatologi Indonesia Vol 17 No 3 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.3.103-112

Resistance of Several Hibiscus cannabinus genotypes Against Meloidogyne incognita Kenaf (Hibiscus cannabinus) is known as a source of natural fibers. Infection of Meloidogyne incognita (root-knot nematode) in kenaf plants causes stunting of plants, thereby reducing crop production. This study aimed to determine the resistance of seven kenaf genotypes against M. incognita. The experiment was conducted by infesting kenaf plants aged 15 days after planting (DAP) with M. incognita in a population of 40 juvenile nematodes 2 per 100 g of soil. The planting medium used was sandy soil with a composition of 55% sand, 36% dust, and 17% clay. The resistance variable consisted of root knot index and nematode reproduction factors. Analysis of salicylic acid, phenol, lignin and several plant growth variables were carried out at 75 DAP. Among the seven kenaf plant genotypes evaluated, there were 3 tolerant genotypes (KR4, KR15 and KR5) and 4 highly susceptible genotypes (KR1, KR6, Kin2, and DS028). Genotypes that had a tolerant response to M. incognita showed an increase in phenolic compounds, salicylic acid, and lignin in the roots compared to the control. The decrease in plant height, crown fresh weight, and root fresh weight varied due to M. incognita infection.

Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni) Parnidi Parnidi; Aprilia Ridhawati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 12, No 1 (2020): APRIL 2020
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/btsm.v12n1.2020.45-53

Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari - Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm.ABSTRACTMicropropagation of Stevia rebaudiana (Bertoni)Stevia is one of the plants that produces natural sweeteners. Stevia micropropagation through tissue culture can provide a large of amount and fast plant material needed for stevia plantation. Micropropagation through tissue culture requires a proper media composition. This study aims to examine the composition of media suitable for stevia micropropagation. The study was conducted at the Tissue Culture Laboratory, Indonesian Sweeteners and Fiber Crops Research Institute in February - June 2016; using a completely randomized design (CRD) with three replicates. The treatment for shoot induction using Murashige and Skoog (MS) basic medium plus Benzyl Amino Purin (BAP) with concentrations: 0; 0.25; 0.5; 0.75 and 1 mg/L. The treatment for root induction using MS basic medium with the addition of 1; 1,5; 2 and 2,5 mg/L IAA, IBA and NAA and for control using MS basic medium without the addition of plant growth regulators. The results showed that the best growth of stevia shoots with mean of shoots number 17.80 and mean of shoots length 3.25 cm was found in MS basic medium + BAP 0.5 mg/L. The best stevia root growth with mean of root number 4.60 and mean of root length 2.27 cm was found in MS basic medium + IAA 1 mg/L.

Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni) Parnidi Parnidi; Aprilia Ridhawati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 12, No 1 (2020): APRIL 2020
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari - Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm.ABSTRACTMicropropagation of Stevia rebaudiana (Bertoni)Stevia is one of the plants that produces natural sweeteners. Stevia micropropagation through tissue culture can provide a large of amount and fast plant material needed for stevia plantation. Micropropagation through tissue culture requires a proper media composition. This study aims to examine the composition of media suitable for stevia micropropagation. The study was conducted at the Tissue Culture Laboratory, Indonesian Sweeteners and Fiber Crops Research Institute in February - June 2016; using a completely randomized design (CRD) with three replicates. The treatment for shoot induction using Murashige and Skoog (MS) basic medium plus Benzyl Amino Purin (BAP) with concentrations: 0; 0.25; 0.5; 0.75 and 1 mg/L. The treatment for root induction using MS basic medium with the addition of 1; 1,5; 2 and 2,5 mg/L IAA, IBA and NAA and for control using MS basic medium without the addition of plant growth regulators. The results showed that the best growth of stevia shoots with mean of shoots number 17.80 and mean of shoots length 3.25 cm was found in MS basic medium + BAP 0.5 mg/L. The best stevia root growth with mean of root number 4.60 and mean of root length 2.27 cm was found in MS basic medium + IAA 1 mg/L.

PENDUGAAN PEWARISAN SIFAT KETAHANAN KENAF TERHADAP NEMATODA PURU AKAR (Meliodogyne incognita) Parnidi Parnidi; Aditya Aji Noftara; Mala Murianingrum; Taufiq Hidayat RS; Marjani Marjani
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 2 (2022): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i2.1211

Root puru nematodes (Meloidogyne spp.) on kenaf plants are known as one of the harmful plant-disturbing organisms. NPA infection in roots causes plants to grow stunted and production potential decreases. The inheritance of kenaf resistance to NPA M. incognita was carried out using a sixpopulation approach (P1, P2, F1, F2 BC1P1 and BC1P2). The genotype (Kenafindo Agribun 2 = Kin2) which was highly susceptible to NPA M. incognita was used as the female parent and the tolerant genotype (Karangploso 15 = KR15) as the male parent. The nature of kenaf resistance to NPA M. incognita was based on the variable number of shells, reproductive factors, number of 2nd instar juveniles, number of egg masses and number of eggs per egg mass. Based on the results of the study showed that the resistance of kenaf to NPA M. incognita showed that it was not influenced by female parents. The number of resistance genes is controlled by one gene. The character of kenaf resistance to NPA M. incognita was controlled by a partially positive dominant gene. The action of genes controlling resistance to NPA M. incognita was additive-dominant. The heritability value in the broad sense and the heritability value in the narrow sense for all variables of kenaf resistance to NPA M. incognita were high

The Influence of Leaf Harvesting Methods on Tobacco Seed Production and Quality Parnidi Parnidi; Imam Santoso; Mochamad Sohri; Dian Hariyanto; Anik Herwati
Jurnal Penelitian Pendidikan IPA Vol 9 No 7 (2023): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i7.2580

To produce high-quality seeds, the harvest must be carried out on time so that the seed maturity is almost uniform. Like wise to produce seeds of high physiological quality. The study was conducted at the Karangploso Experimental Garden, Balittas Malang, in January-October 2017. The varieties used were Prancak-N1, with 6 treatments namely: (1) Leaves harvested and shredded, (2) Leaves harvested and shredded are not discarded, (3) Leaves not harvested and suckers not removed (4) Leaves not harvested and shredded (5 koseran leaves not harvested shingles disposed and (6) Koseran leaves harvested and shredded discarded.The treatment was planted as many as 10 polybags with a size of 20 kg of soil, one plant per polybag, population of each treatment as many as 10 plants with 3 replications. The fertilizer used was NPK fertilizer with a dose of 15 grams per plant. Analysis used Randomized Group Design repeated 3 times, to calculate the variance using the Duncan Distance Test (DMRT) with a level of 5%. The purpose of the study was to determine the effect of the treatment of harvesting leaves and tobacco flowers on the production and quality of the seeds produced. The results showed that the highest seed production from the six treatments was treated with leaves not harvested and shredded, the highest production of seeds reached 17.4 grams, but not significantly different from the treatment of koseran leaves not the harvested sirung was disposed of (16.10 grams) and the coseran leaves were harvested, the flush was disposed of (15.70 grams). For the highest seed vigor test, the leaves were not harvested and the flush was disposed (17.40%), while the highest germination test for the flush treatment was reached (93.33%), but not significantly different from the treatment of the leaf leaves not harvested by sirung discarded (91.33%) and the treatment of koseran leaves was harvested, flush was disposed (84.68%). Observation of abnormal sprouts from the six treatments was not significantly different between 1.33%-2.33%

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