Afiqah Yaacob
Universiti Tun Hussein Onn Malaysia

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Uric acid detection in visible spectrum Afiqah Yaacob; Nor Hafizah Ngajikin; Nurfatihah Che Abd Rashid; Siti Hajar Aminah Ali; Maslina Yaacob; Suhaila Isaak; Noran Azizan Cholan
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 18, No 4: August 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v18i4.14993

Abstract

The measurement of uric acid based on the optical absorption at visible light spectrum is investigated and tested. Sensing in the visible region was conducted for determination of suitable wavelength that produces high sensitivity and accuracy performance based on the Beer-Lambert law calculation. In this work, the uric acid is detected by detecting sodium urate as a product of chemical reaction between uric acid with sodium hydroxide buffer. The setup has been tested for uric acid concentration ranging from 15 mg/dL to 85 mg/dL. Three wavelengths have been analyzed which are 460 nm, 525 nm and 630 nm. Measured data at 460nm wavelength exhibits the highest sensitivity, which is 0.0012 (mg/dL)-with 86.51% accuracy. Detection of uric acid at visible light spectrum offers a low-cost sensor based on visible LEDs and photodiode is possible to be realized.
Optimization of light source wavelength for ammonia detection in water Nurfatihah Che Abd Rashid; Noran Azizan Cholan; Kim Gaik Tay; Afiqah Yaacob; Nazrah Ilyana Sulaiman; Khairulanwar Mokhiri; Nor Hafizah Ngajikin
TELKOMNIKA (Telecommunication Computing Electronics and Control) Vol 20, No 5: October 2022
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/telkomnika.v20i5.24079

Abstract

Optimization of light source wavelength for ammonia detection in surface water is presented in this work. For the ammonia detection, the surface water sample is mixed with sodium chloride and nessler reagent, whereas the sensor head consists of unclad plastic optical fiber. The unclad region has a length of 1 cm and the cladding is removed by immersing it in acetone solution. Experimental results indicate that the output light intensity of the sensor has linear relationship with the ammonia concentration. At the wavelength of 510 nm, the output light increases linearly as the ammonia concentration varies from 0.07 mg/L to 8.97 mg/L. At the same wavelength, the proposed sensor achieves the sensitivity of 0.0139 (mg/L)-1, accuracy of 99.59% and resolution of 0.72 µg/L. The analysis of light source wavelength reveals that a wavelength range from 450 nm to 580 nm produces the optimized performances. Within this wavelength range, the proposed sensor achieves sensitivity of higher than 0.01 (mg/L)-1, accuracy of higher than 99% and resolution of less than 1 µg/L.