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All Journal Biotika: Jurnal Ilmiah Biologi Cropsaver : Journal of Plant Protection
Mia Miranti Rustama
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) Melanie Melanie; Mia Miranti Rustama; Inriyani Sintia Sihotang; Hikmat Kasmara
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.668 KB) | DOI: 10.24198/cs.v1i1.16999

Abstract

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation
EFEKTIVITAS FORMULASI Helicoverpa armigera Nuclear Polyhedrosis Virus SUBKULTUR (HaNPV1 ) DALAM BERBAGAI BAHAN PEMBAWA TERHADAP MORTALITAS DAN PERTUMBUHAN Crocidolomia pavonana Fabricius Amalia Fildzah Tamii; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10073

Abstract

EFEKTIVITAS FORMULASI Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) HASIL SUB-KULTUR TERHADAP BERAT MAKSIMUM DAN LOLOS HIDUP LARVA Plutella xylostella Fuji Hardiani; Melanie Melanie; Mia Miranti Rustama
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10074

Abstract

EFEKTIVITAS FORMULASI Helicoperva armigera NUCLEAR POLYHEDROSIS VIRUS HASIL SUB KULTUR (HaNPV ) TERHADAP MORTALITAS, WAKTU KEMATIAN DAN BERAT BADAN LARVA Spodoptera litura Fabricius (1775) Fitriyani Fitriyani; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10079

Abstract

Pengaruh Suhu Pengeringan Polyhedra Terhadap Efektivitas Helicoverpa Armigera Nuclear Polyhedrosis Virus (HaNPV) Korespondensi: Dr. Mia Miranti, MP. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran, emal : miamiantariksa@gmail.com. Subkultur dalam Mengendalikan Populasi Spodoptera litura Fabricius Putri Marissa; Mia Miranti Rustama; Wawan Hermawan
BIOTIKA Jurnal Ilmiah Biologi Vol 11, No 1 (2013): BIOTIKA JUNI 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v11i1.10041

Abstract

Penelitian mengenai pengaruh suhu pengeringan polyhedra terhadap efektivitas Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) subkultur dalam mengendalikan populasi Spodoptera litura Fabricus telah dilakukan. Penelitian dilakukan dengan metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal dengan 5 taraf perlakuan (HaNPV cair dan HaNPV yang dikeringkan pada suhu 4oC, 25C) dengan empat kali pengulangan. Hasil penelitian menunjukkan bahwa berat badan maksimum larva S. litura instar tiga mencapai 0,967 g terjadi pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C dan pertambahan berat badan mencapai 0,732 g/ekor/hari pada larva yang terinfeksi HaNPV yang dikeringkan pada suhu 4C
Co-Authors Amalia Fildzah Tamii Fitriyani Fitriyani Fuji Hardiani Hikmat Kasmara Inriyani Sintia Sihotang Melanie Melanie Putri Marissa Wawan Hermawan