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All Journal Biotika: Jurnal Ilmiah Biologi Cropsaver : Journal of Plant Protection
Melanie Melanie
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) Melanie Melanie; Mia Miranti Rustama; Inriyani Sintia Sihotang; Hikmat Kasmara
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.668 KB) | DOI: 10.24198/cs.v1i1.16999

Abstract

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti.  B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti  resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis  that  non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation
EFEKTIVITAS FORMULASI Helicoverpa armigera Nuclear Polyhedrosis Virus SUBKULTUR (HaNPV1 ) DALAM BERBAGAI BAHAN PEMBAWA TERHADAP MORTALITAS DAN PERTUMBUHAN Crocidolomia pavonana Fabricius Amalia Fildzah Tamii; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10073

Abstract

INFEKSI JAMUR ENTOMOPATOGEN Metarhizium anisopliae (Sorokin) (1883) YANG DIAPLIKASIKAN BERSAMA SENYAWA ANDROGRAFOLIDA TERHADAP MORTALITAS LARVA Crocidolomia pavonana (Fabricus) (1794) Ghina Ganiyya; Melanie Melanie; Hikmat Kasmara
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10083

Abstract

EFEKTIVITAS FORMULASI Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) HASIL SUB-KULTUR TERHADAP BERAT MAKSIMUM DAN LOLOS HIDUP LARVA Plutella xylostella Fuji Hardiani; Melanie Melanie; Mia Miranti Rustama
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 1 (2014): BIOTIKA JUNI 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i1.10074

Abstract

Uji Toksisitas Sedimen Sungai Citarum Terhadap Mortalitas Larva Hydropsyche Sp. (Trichoptera : Hydropsychidae) Desy Aryani Aryani; Sunardi Sunardi; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 11, No 2 (2013): BIOTIKA DESEMBER 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v11i2.10058

Abstract

EFEKTIVITAS FORMULASI Helicoperva armigera NUCLEAR POLYHEDROSIS VIRUS HASIL SUB KULTUR (HaNPV ) TERHADAP MORTALITAS, WAKTU KEMATIAN DAN BERAT BADAN LARVA Spodoptera litura Fabricius (1775) Fitriyani Fitriyani; Mia Miranti Rustama; Melanie Melanie
BIOTIKA Jurnal Ilmiah Biologi Vol 12, No 2 (2014): BIOTIKA DESEMBER 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v12i2.10079

Abstract

PENGENDALIAN LARVA NYAMUK Aedes aegypti DENGAN MEMANFAATKAN POTENSI METABOLIT TUMBUHAN DAN AGENSIA ENTOMOPATOGEN Melanie Melanie; Wawan Hermawan Wawan Hermawan; Hikmat Kasmara Hikmat Kasmara
BIOTIKA Jurnal Ilmiah Biologi Vol 17, No 1 (2019): BIOTIKA JUNI 2019
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v17i1.23227

Abstract

POTENSI INSEKTISIDA NABATI MENGANDUNG EUGENOL DAN SITRONELLAL DALAM PENGENDALIAN Helopeltis antonii HAMA TANAMAN Anacardium occidentale Melanie Melanie; Aidha Utami; Hikmat Kasmara; I Wayan Laba
BIOTIKA Jurnal Ilmiah Biologi Vol 14, No 1 (2016): BIOTIKA JUNI 2016
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v14i1.14409

Abstract

Telah dilakukan penelitian mengenai potensi insektisida nabati yang mengandung Eugenol dan Sitroenellal terhadap Helopeltis antonii (Hemiptera: Miridae) hama pada tanaman jambu mete (Anacardium occidentale). Tujuan dari penelitian ini adalah untuk mengetahui efektivitas dan daya simpan insektisida nabati yang mengandung Eugenol dan Sitronellal untuk mengendalikan hama H. antonii dan tingkat serangan hama tersebut terhadap tanaman jambu mete. Metode yang digunakan adalah eksperimental dengan uji hayati di Rumah Kaca laboratorium uji hayati Balitro, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri atas dua faktor, yaitu waktu simpan dengan 5 taraf dan insektisida yang digunakan 6 taraf, dilakukan sebanyak 4 ulangan. Aplikasi dilakukan dengan metode semprot serangga dan semprot pakan. Parameter yang diamati adalah mortalitas hewan uji dan tingkat serangan pada tanaman. Data dianalisis menggunakan uji sidik ragam dengan taraf kepercayaan 95%. Apabila terdapat perbedaan nyata, analisis dilanjutkan dengan menggunakan uji jarak berganda dengan taraf nyata 5%. Hasil penelitian menunjukkan meningkatnya mortalitas H. antonii di minggu ke-6, ke-8, dan ke-10 akibat aplikasi Eugenol dan di minggu ke-6, hingga ke-12 pada aplikasi Sitronellal, tingkat kerusakan pada tanaman menurun pada minggu ke-6 hingga ke-10 dengan metode semprot pakan. Aplikasi metode semprot serangga, Sitronellal meningkatkan mortalitas H. antonii di minggu ke-6 hingga ke-12, dan di minggu ke-6 dan ke-8 pada penggunaan Eugenol, tingkat kerusakan menurun mulai minggu ke-6 hingga ke-12.
Co-Authors Aidha Utami Amalia Fildzah Tamii Aryani, Desy Fitriyani Fitriyani Fuji Hardiani Ghina Ganiyya Hikmat Kasmara Hikmat Kasmara Hikmat Kasmara I WAYAN LABA Inriyani Sintia Sihotang Mia Miranti Rustama sunardi sunardi Wawan Hermawan