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All Journal Jurnal Teknologi Perikanan dan Kelautan
Jeffri A Mandeno
Jurusan Perikanan dan Kebaharian, Politeknik Negeri Nusa Utara, Tahuna
Agriculture, Biological Sciences & Forestry Education Library & Information Science

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ISOLASI DAN IDENTIFIKASI BAKTERI STAPHYLOCOCCUS EPIDERMIS PADA IKAN ASAP PINEKUHE Ely John Karimela; Frans G Ijong; Jaka F P Palawe; Jeffri A Mandeno
Jurnal Teknologi Perikanan dan Kelautan Vol 9 No 1 (2018): MEI 2018
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1821.537 KB) | DOI: 10.24319/jtpk.9.35-42

Abstract

Pinekuhe smoked fish is a fishery product traditionally processed by fishermen of Sangihe Island Regency. Staphylococcus epidermis is an opportunistic phatogen that causes infection to humans of weak immunity. This study aims to identify S. epidermis isolated from the Pinekuhe smoked fish. Fourty isolate samples were grown on Mannitol salt Agar medium and suspect colonies were tested for Gram staining, Catalase, Motility and Coagulase test There were 13 isolates identified as S. epidermis which characteristics as gram-positive, round shapes, clustering, 0,5μm-1μm in diameter, non-motilepositive catalase, and not ferment Mannitol. So, there were 32.5% S. epidermis that contaminated Pinekuhe smoked fish.
TINGKAT KONTAMINASI MIKROBA PADA BEBERAPA UNIT PENGOLAHAN IKAN ASAP PINEKUHE DI KABUPATEN SANGIHE Ely John Karimela; Jeffri A Mandeno
Jurnal Teknologi Perikanan dan Kelautan Vol 10 No 1 (2019): MEI 2019
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2261.801 KB) | DOI: 10.24319/jtpk.10.61-68

Abstract

One of the products processed by smoked fisheries owned by fishermen regency of Sangihe Island is Pinekuhe. This research aims to determine the level of microbial contamination in the smoked fish Pinekuhe processed fishermen regency of Sangihe Islands. Sampling was taken randomly in some of the fish processing units of Pinekuhe smoke. Observation of the deterioration of the quality of fish products Pinekuhe, observed through the test of Total Plate Count (TPC), total mould, and total of Staphylococcus sp. The results showed that the TPC value of all processors meets SNI standards. TPC value of A, B, C, and D processors, respectively, 1,3 x 104 CFU/G, 2,6 x 104 CFU/G, 6,9 x 104, and 1,2 x 104 CFU/g. The total observation of Staphylococcus sp. on processor A and processor B generates a value of 0, while processor C has a total value of 1.1 x 102 TVC/G and processor D has a total value of Staphylococcus sp. 1,2 x 102 TVC/g. The total number of bacteria, the total amount of mould, and the total number of Staphylococcus sp. Still qualify SNI, except Staphylococcus sp. on processor C and processor D exceeds the amount required by the Indonesian National Standard on the limits of contamination microbes in smoked fish.
Co-Authors Ely John Karimela Frans G Ijong Jaka F P Palawe