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All Journal Jurnal Ilmu Pertanian Indonesia Hemera Zoa Indonesian Journal of Biotechnology
Retno D. Soejoedono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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PEMBUATAN DAN STANDARISASI ANTIGEN AI H5N1 KOMERSIAL UNTUK MONITORING TITER ANTIBODI HASIL VAKSINASI AI DI INDUSTRI PETERNAKAN AYAM Retno D. Soejoedono; Sri Murtini; Kamalludin Zarkasie
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Vaccination is one of the chosen strategy for controling AI H5N1 in Indonesia. Vaccination able to induce protective antibodies against AI but unable to inhibit viral infection. Determination of antibody titers in the serum from bird vaccinated with AI-H5N1 vaccine consisting of 2 or 3 different AI virus isolates difficult to be meassured if the antigen for HI test is uncalibrated yet. Furthermore, the determination of a minimum protective antibody titer against the challenge of AI virus circulating in the field at this time needs to be done.  This study aims to determine the H5N1 AI virus antigen for standart HI test and the minimum titre of antibodies that able neutralize virus infection. As much as 55 chickens were divided into 11 groups, 10 groups vaccinated with commercial AI vaccine and AI H5N1 field isolat antigen. Four types of commercial vaccines were veccinated to one group and seven other groups vaccinated with the antigen AI Legok 2004, Nagrak Ag 2009, Ag Lawang 2010, as well as polyvalent Ag combination of these three types of antigen. After third vaccinations, the presence of antibodieswere meassured by HI test. Serum with a titer test 26-28 were tested for the capability of virus neutralizationin using virus neutralization test against three different H5N1 AI virus field isolates. The test results showed that the H5N1 subtype AI virus antigen representative as standart antigen for HI test is antigen Legok 2004 and the minimum titer which able neutralize H5N1 AI virus field isolates 28.
Penggunaan teknik reaksi rantai polimerase (PCR) DNA proviral dari virus enzootic bovine leukosis Retno D. Soejoedono
Hemera Zoa Vol. 74 No. 3 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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A polymerase chain reaction was developed to detect the proviral DNA of bovine leukimia virus in bovine lymphocytes. Milk and blood samples from infected and uninfected cows were tested. None of the bovine leukosis free animals gave a positive response and 58% of the infected cows showed positive signals. When the DNAs 25ul of blood. 25.000 lymphocytes or 1.000 lymphocytes were amplified. positive results were recorded more frequently in cows with persistent  lymphocytes than in cows with normal  lymphocyte counts.
Serological investigation on swollen head syndrome in Indonesia Aenuh R. Jusa; Retno D. Soejoedono; Cattleya S. Leksmono; Mastur A.R. Noor; Syamsul B. Siregar; Madsuki Pertadiredja
Hemera Zoa Vol. 75 No. 3 (1992): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Clinical signs indicated that Swollen Head Syndrome (SHS) was suspected to be present in Indonesia. Serological investigation was carried out by collecting a number of 242 serum samples from Tangerang, Bogor, Cianjurm Ciawi, Cicurug dan Magelang, tested using serum neutralization (SN) test. The results of SN test indicated that SHS has been identified in these investigation areas. Based on the fact that these are the center for parent stock breeders it could be suspected taht the disease might have been speread to several areas of chicken farms in this country.It would be wise if a control program be formulated since this time, to ensure that everything is well prepared in case SHS situation develops into unfavorable condition.
Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein R. Susanti; Retno D. Soejoedono; I Gusti Ngurah K Mahardika; Wayan T I Wibawan; Maggy T Suhartono
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.287 KB) | DOI: 10.22146/ijbiotech.7803

Abstract

Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. Thisresearch aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Javabased on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HAgenes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, andsequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignmentof nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns ofamino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all ofthe viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.Keywords: cleavage site, waterfowls, HPAI
Co-Authors Aenuh R. Jusa Cattleya S. Leksmono I Gusti Ngurah K Mahardika Kamalludin Zarkasie Madsuki Pertadiredja Maggy T Suhartono Mastur A.R. Noor R. Susanti sri murtini . Syamsul B. Siregar Wayan T I Wibawan