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All Journal Jurnal Ilmu Pertanian Indonesia Indonesian Journal of Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Biology Education Indonesian Journal of Mathematics and Natural Sciences Life Science Berdaya Indonesian Journal of Community Empowerment
R. Susanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Social Sciences Other

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Serologi dan virologi virus avian influenza H5N1 pada kucing jalanan di Kota Bogor Sri Murtini; R. Susanti; Ekowati Handharyani
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1426.848 KB)

Abstract

Highly  pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen in birds. Recently, the virus has been reported to cause sporadic fatal disease in tigers, leopards, and other exotic felids as well as domestic cats  in Thailand. The present study was carried out to investigate the presence of AI H5N1 virus infection in stray cats roaming around residential, traditional and chicken farms in Bogor, West Java. Ninety serum samples were tested using HI test to screened for the presence of antibody to AI H5N1. Virus isolation was done in SPF embrionated chicken eggs and identify using HI, AGP and RT-PCR. The results showed that 18,9% of stray cats developed antibodies against H5 with geometric mean titre 23,1 . Stray cats lived in traditional markets 18–40% developed antibodies in the titre ranging from 22,8  to 24,5. Only two out of nine stray cats which lived in chicken farm developed low antibody titres again H5 (21). None of the stray cats lived in residencial area have developed antibodies against H5. This study revealed that stray cats have been contact with AI H5. Avian influenza H5 viruses were isolated in eight out of 33 pooled of rectal swab samples. The viral cleavage site sequences are CCTCAAAGAGAGAGC AGAAGAAAGAAGAGAGGT which represent amino acid sequences of PQRESRRKKRG. Based on the cleavage site sequence, the isolates are similar with the AI H5 virus subtype isolated from human in Indonesia during 2005–2007.
Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein R. Susanti; Retno D. Soejoedono; I Gusti Ngurah K Mahardika; Wayan T I Wibawan; Maggy T Suhartono
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.287 KB) | DOI: 10.22146/ijbiotech.7803

Abstract

Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. Thisresearch aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Javabased on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HAgenes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, andsequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignmentof nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns ofamino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all ofthe viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.Keywords: cleavage site, waterfowls, HPAI
Studi Perilaku, Palatabilitas Pakan dan Bentuk Sarang Kesukaan Gelatik Jawa (Padda oryzivora) R. Susanti; Margareta Rahayuningsih; Nugroho Edi Kartijono; Andi Haryoko; Arif Rahman Hakim; Tri Oktaviantari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.147

Abstract

A study was conducted to disclose the habit, food type palatability, and the nest type preference of gelatik jawa (Padda oryzivora). As many as 54 gelatik jawas from Surabaya, Malang, Ngawi, Bekasi, Karawang, Cirebon, Bogor, Semarang, Magelang, Yogyakarta and Prambanan were captured using soft nylon net and then caged for two weeks for adaptation. Each bird was given a ring to make it easier to identify. After the adaptation period, the birds were moved to a wider cage. Every day the habit and mating behaviour as well as the preference of food and nest were observed. The highest  frequency of daily feeding (997.75 ± 72.23) and moving (795.50 ± 138.27); and the lowest  frequency of daily self care (4.126 ± 138.27) and communicate (93.75 ±  7.59) were recorded at 08.00 – 10.00. Food palatability and preference over nest, habit, and biological clock of gelatik jawa could be the basis of ex-situ conservation. The monogamy character of gelatik jawa could also serve as the basis of ex-situ conservation that male and female in one cage must be in equal number.
Study In Silico of the Activity of Bioactive Compounds from Cinnamomum (Cinnamomum verum) Bark as Anti-inflammatory Islami, Prima Gita; R. Susanti
Indonesian Journal of Mathematics and Natural Sciences Vol. 48 No. 1 (2025): Volume 48 Nomor 1 Tahun 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jdcd5m11

Abstract

Cinnamon bark (Cinnamomum verum) contains various bioactive compounds that can be used in drug development, one of which is anti-inflammatory. Inflammation is the body's defense  response to the occurrence of an injury from objects unknown to the body, such as bacterial  infections, trauma, autoimmune, viruses and toxins. The purpose of this study was to determine the content of bioactive compounds in cinnamon bark. This research method was carried out using the Dr. Duke's Phytochemical database. Duke's Phytochemical database to collect  bioactive compounds in cinnamon bark, PubChem to download the ligand structure of bioactive compounds, PASS Online for screening the anti-inflammatory activity value of bioactive  compounds, SEA and Swiss Target Prediction are used to determine the target protein, STRING is used to analyze the interaction between target proteins, Cytoscape is used to predict tissue  topology, Pyrex is used for molecular docking, and Biovia Discovery Studio is used to visualize  docking results. There are 16 cinnamon bark compounds that have a Pa value > 0.5. Based on the docking results, compounds that have a Pa value> 0.5. Based on docking results, beta caryopyhllene, caryopyhllene, fatty acid, and isocaryopyhllene compounds have PPARA activator mechanism with binding affinity of -7.4 kcal/mol, -7.9 kcal/mol, -7.8 kcal/mol, and -5.6  kcal/mol. Caffeic acid compounds have an MMP-9 activator mechanism with a binding affinity of -5.7 kcal/mol. Visualization of docking results shows that isocaryopyhllene and caffeic acid compounds have similar interactions and amino acid residues with control drugs so that they can be used as anti-inflammatory agents. 
Efek Ekstrak Buah Belimbing Wuluh terhadap Kadar SOD Paru Tikus yang Dipapar Asap Rokok: Effect of Starfruit Extract on SOD Levels in the Lungs of Rats Exposed to Cigarette Smoke Setyaningsih, Anggun Try; R. Susanti
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.22267

Abstract

  Cigarette smoke contains a variety of chemical compounds that are harmful to more than 4000 particles that are particularly harmful to cell metabolism such as tar, nicotine, and CO. Cigarette smoke is a free radical that can cause oxidative stress if excessively inhaled in the lungs. Oxidative stress can be characterized by a decrease in SOD levels. SOD enzymes are naturally occurring antioxidants produced in the body to neutralize free radicals. This study aims to find out the effect of Averrhoa bilimbi fruit extract on the lung SOD level of male rats exposed to cigarette smoke. This study used 25 male rats divided into five groups, namely the normal (K) group, the negative group given exposure to cigarette smoke (K-), the treatment group given exposure to cigarette smoke and the extract A. bilimbi fruit 3 mg/kgBB (K1), treatment group given exposure to cigarette smoke and fruit extract A. bilimbi 6 mg/kgBB (K2), treatment group given exposure to cigarette smoke and fruit extract of A. bilimbi 12 mg/kgBB (K3). The antioxidant content of the A. bilimbi fruit extract is analyzed using the DPPH method and the SOD level of the lungs is measured by the calorimetric method. Analysis of SOD data using One Way Anova test and LSD test. The results of the antioxidant test of the A. bilimbi fruit extract showed a 23.37% antioxidant status. The results of statistical tests of A. bilimbi fruit extract on lung SOD levels exposed to cigarette smoke have a real effect. Each group showed real differences in the form of K (79.99%), K- (21.07%), K1 (52.14%), K2 (64.29%), K3 (72.86%). From the results of the research that has been carried out, it can be concluded that the fruit extract of A. bilimbi has an effect on the increase in the SOD level of mice exposed to cigarette smoke.  
Learning Ecosystem Material with Project Based Learning (PjBL) Model to Improve Science Process Skills and Student Creativity Intan Rita Savitri; R. Susanti
Journal of Biology Education Vol. 13 No. 2 (2024): Agustus 2024
Publisher : Universitas Negeri Semarang

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Abstract

This research is a quasi-experiment with a non-equivalent control group design. The samples in this study were X1 and X2 grade students who were determined by cluster purposive sampling. Data collection techniques were carried out by distributing pretest and posttest. Data analysis used in this research is normality test, homogeneity test, independent sample t-test, and n-gain test. The results of this study showed that ecosystem learning with Project Based Learning (PjBL) model can improve science process skills and creativity. The N-Gain test results of science process skills of control class students were 0.30 which was categorized as low and the experimental class was 0.64 which was categorized as moderate. While the N-Gain test of student creativity in the control class was 0.29 which was categorized as low and the experimental class was 0.58 which was categorized as moderate. The conclusion of this research is that learning ecosystem material with the PjBL model can improve students' science process skills and creativity. Suggestions in this study are that teachers can apply the PjBL model to optimize student learning outcomes in other materials that are in accordance with the characteristics of the model and the PjBL learning model can be applied to biology learning with a wider scope of material.
The Effect of Tamarind Fruit Nanoparticles (Tamarindus indica) on the Sperm Quality of Hyperglycemic Rats Bagus Hammam Falah; Wulan Christijanti; Nugrahaningsih WH; R. Susanti
Life Science Vol. 14 No. 2 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i2.24064

Abstract

Hyperglycemia is an increase in glucose levels in the blood. This condition can trigger glucose autooxidation which can produce free radicals. Free radicals can cause damage to male reproductive organs and quality. Tamarind fruit has a high flavonoid which has the potential as an antioxidant. This study aims to analyze the effect of tamarind fruit nanoparticles on spermatozoa quality in hyperglycemic rats. This study is experimental with Post-Test Control Group Design. A sample of 15 male Wistar rats were induced by alloxan and then divided into three groups, namely the control group, tamarind fruit nanoparticles with a dose of 30 mg/KgBB (NP1) and 60 mg/KgBB (NP2) for 28 days. Sperm quality was observed with parameters of concentration, motility and viability. The data were analyzed using One Way ANOVA and LSD. The results of the Anova test showed that tamarind nanoparticle treatment could significantly improve sperm quality (P<0.05). The LSD test showed that the concentration, motility and viability of sperm in group (K) were significantly different from groups NP1 and NP2. The highest to lowest average sperm concentration in sequence was in groups NP2 (49.60x106±6.06x106), NP1 (41.60x106±7.40x106) and K (20.80x106±7.56x106). The highest to lowest average percentage of sperm motility in sequence was in groups NP2 (82.20±9.75), NP1 (74.00±4.74), K (47.20±10.66). The highest to lowest average percentage of sperm viability in sequence was in the NP2 group (76.80±4.49), NP1 (72.20±7.12), K (31.60±7.89). The conclusion of this study is that tamarind nanoparticles have an effect on improving sperm quality in hyperglycemic rats.
Penyiapan Siswa Menghadapi Asesmen Kompetensi Minimum Ani Rusilowati; Masrukan Masrukan; R. Susanti; Triastuti Sulistyaningsih; Tri Sri NoorAsih; Heru Setyanto
BERDAYA Indonesian Journal of Community Empowerment Vol. 3 No. 1 (2024)
Publisher : Universitas Negeri Semarang

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Abstract

Elementary school teachers in Gunungpati District, Semarang, still experience difficulties in: (1) implementing learning that can prepare students to face the minimum competency assessment (AKM), (2) compiling instruments that can be used to train students to face AKM, namely literacy and numeracy. Therefore, it is necessary to carry out training activities for teachers in the KKG, in order to prepare them to familiarize their students with knowing and taking literacy and numeracy tests. The aim of this service activity is to provide training for teachers through KKG activities with a facilitation-based Action Learning model. The specific aim is to assist elementary school teachers in improving their pedagogical competence in the areas of learning and evaluation. The problem solving method that has been agreed upon by partners is the implementation of programmed training. The solution method design refers to the facilitation-based Action Learning model. Action learning is defined as a process in which a group of people come together regularly, help each other to learn, and share experiences. This training requires a tutor who acts as a facilitator, and continues with the mentoring phase. Before carrying out the training, a Focus Group Discussion (FGD) was held with several elementary school principals and the head of the Dewi Kunthi KKG, Gunungpati District, Semarang. The results of the FGD are used to design training scenarios for each activity and training materials. The success of the training was marked by the increasing ability of teachers in designing science and science lessons and preparing AKM questions, namely literacy and numeracy.
Co-Authors Ali Djamhuri Andi Haryoko Ani Rusilowati Bagus Hammam Falah Ekowati Handharyani Heru Setyanto I Gusti Ngurah K Mahardika Intan Rita Savitri Islami, Prima Gita Maggy T Suhartono Margareta Rahayuningsih Masrukan Masrukan Nugrahaningsih WH Nugroho Edi Kartijono Retno D. Soejoedono Setyaningsih, Anggun Try sri murtini . Tri Oktaviantari Tri Sri NoorAsih Triastuti Sulistyaningsih Wayan T I Wibawan Wulan Christijanti