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All Journal Agrointek Jurnal Penelitian Sains
Yadi Suryadi
ICABIOGRAD-IAARD
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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Pengaruh Rasio Kitosan-Sodium Tripolifosfat Terhadap Pengendalian Antraknosa (Colletotrichum gloeosporioides) Pada Mangga Kultivar Manalagi Yadi Suryadi; Dwi N. Susilowati; I. M. Samudra
Jurnal Penelitian Sains Vol 22, No 3 (2020)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.574 KB) | DOI: 10.56064/jps.v22i3.581

Abstract

Anthracnose disease caused by the fungus Colletotrichum gloeosporioides is one of the most important diseases on fruit trees. Chitosan material have been applied to many different application fields including disease biocontrol because of its  biocompatible and biodegradable effect. This research was aimed  to synthesize low molecular weight chitosan (LMWC) which prepared via enzymatic process using a bacterium Burkholderia cepacia E76 and ionic gelation method, using sodium tripolyphosphate (NaTPP), and to test their activity against C. gloeosporioides on mangoes fruits. In this study the effect of concentration and volume ratio of LMWC : NaTPP was determined based on particle characteristics, i.e particle size, polydispersity index, zeta potential and FTIR structure. The results showed that LMWC could be synthesized by means of enzymatic hydrolysis using chitinase originated from B. cepacia E76. The optimal nanoparticle conditions was obtained by concentration of 0.3% LMWC, 0.1% TPP with volume ratio 5 : 1, stirring for 30-60 min incubation with the particle size of  126.2 nm and ZP value of  25.5 + 6.1 mV. LMWC and nano chitosan product could be evidenced by FTIR analysis. Nano chitosan could inhibit the growth of C. gloeosporioides ranging from 61.28 – 96% under in vitro test.  The result of in vivo test on manggoes fruit cv Manalagi, showed that chitosan nano particle could effectively inhibit the growth of anthracnose (74.5%) at concentration of 0.3% LMWC, and 0.1% NaTPP with ratio 5:2 compared with control. Keywords: B. cepacia E76, chitinase, chitosan, low molecular weight, anti fungi
Biosintesis nanopartikel perak (AgNP) menggunakan Bacillus firmus E65 dan aktivitasnya terhadap mikroba patogen Yadi Suryadi; Dwi Ningsih Susilowati; I Made - Samudra
AGROINTEK Vol 16, No 2 (2022)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v16i2.10785

Abstract

Silver (Ag) in ionic form is toxic to microbial cells, but is environmentally friendly and safe for humans. This study aims to synthesize silver nanoparticles (AgNP) using Gram-positive bacterial isolate (B. firmus E65) as a bioreductor and to test its activity as an antimicrobial against Eschericia coli, Xanthomonas oryzae pv. oryzae (Xoo) and the fungus Colletotrichum gloeosporioides under in-vitro assays. AgNP was obtained by adding bacterial culture supernatant B. firmus E-65 to 5mM AgNO3 solution. The formation of AgNP was observed by changing the color of the solution after incubation at 37 °C for 72 hours. The UV-Vis spectrophotometer measurement to AgNP solution showed a maximum wavelength of 425 nm. The particle size of AgNP was 252.1 nm with intensity of 98.90 %. The result of bioassay against E. coli showed the greatest inhibition at 50 % AgNP (degree of inhibition (DI) =96.15 %), followed by 25 % AgNP (DI=76.92 %), and 12.5 % AgNP (DI=53.84 %). The bioassay against Xoo showed the greatest degree of inhibition was at AgNP 50 % (DI=92.85 %), followed by 25 % AgNP  and 12.5 % AgNP (DI=85.71 %). Meanwhile bioassay against C.gloeosporioides, the greatest inhibition  was observed at 25 % AgNP (DI=94.35 %), followed by 50 % AgNP (DI=91.9 %).
Co-Authors Dwi N. Susilowati Dwi Ningsih Susilowati I Made - Samudra I. M. Samudra