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All Journal Jurnal Penelitian Sains Jurnal Tanah dan Iklim
Dwi N. Susilowati
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Agriculture, Biological Sciences & Forestry Chemistry Mathematics Physics

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Pengaruh Rasio Kitosan-Sodium Tripolifosfat Terhadap Pengendalian Antraknosa (Colletotrichum gloeosporioides) Pada Mangga Kultivar Manalagi Yadi Suryadi; Dwi N. Susilowati; I. M. Samudra
Jurnal Penelitian Sains Vol 22, No 3 (2020)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.574 KB) | DOI: 10.56064/jps.v22i3.581

Abstract

Anthracnose disease caused by the fungus Colletotrichum gloeosporioides is one of the most important diseases on fruit trees. Chitosan material have been applied to many different application fields including disease biocontrol because of its  biocompatible and biodegradable effect. This research was aimed  to synthesize low molecular weight chitosan (LMWC) which prepared via enzymatic process using a bacterium Burkholderia cepacia E76 and ionic gelation method, using sodium tripolyphosphate (NaTPP), and to test their activity against C. gloeosporioides on mangoes fruits. In this study the effect of concentration and volume ratio of LMWC : NaTPP was determined based on particle characteristics, i.e particle size, polydispersity index, zeta potential and FTIR structure. The results showed that LMWC could be synthesized by means of enzymatic hydrolysis using chitinase originated from B. cepacia E76. The optimal nanoparticle conditions was obtained by concentration of 0.3% LMWC, 0.1% TPP with volume ratio 5 : 1, stirring for 30-60 min incubation with the particle size of  126.2 nm and ZP value of  25.5 + 6.1 mV. LMWC and nano chitosan product could be evidenced by FTIR analysis. Nano chitosan could inhibit the growth of C. gloeosporioides ranging from 61.28 – 96% under in vitro test.  The result of in vivo test on manggoes fruit cv Manalagi, showed that chitosan nano particle could effectively inhibit the growth of anthracnose (74.5%) at concentration of 0.3% LMWC, and 0.1% NaTPP with ratio 5:2 compared with control. Keywords: B. cepacia E76, chitinase, chitosan, low molecular weight, anti fungi
Increasing of N-uptake by Inoculation of Diazotroph Endophytic Bacteria in Vermiculite Media DWI N. SUSILOWATI; RATIH SARASWATI; R.D HASTUTI; E. YUNIARTI
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) No 26 (2007): Desember 2007
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v0n26.2007.%p

Abstract

Intensive selection of selected 15 isolates on N2-fixing activities and auxin production to diazotroph endophytic bacteria showed that five isolates were superior, that is KACP12 (0.2569 μmol hour-1 culture-1), KACP13 (0.3026 μmol hour-1 culture-1), KACP21 (0.4592 μmol hour-1 culture-1), KACP32 (0.3131 μmol hour-1 culture-1), and KAMG2 (0.4843 μmol hour-1 culture-1). Inoculated five superior isolates into soybean seeds in vermiculite media showed that soybean plant inoculated by KAMG2 has the highest nitrogenase specific activity compared to others and control, that is 2,54 ± 1,2 μmol hour-1 plant-1. However inoculation with KACM and KACP32 showed higher N-uptake of soybean plant. Although this research has conducted within theearly stage of soybean plant growth, it is obvious that inoculated diazotroph endophytic bacteria in vermiculite media seem to be a good method to introduce selected strain envisaging growth promoting and nitrogen fixation.
Co-Authors E. YUNIARTI I. M. Samudra R.D HASTUTI RATIH SARASWATI Yadi Suryadi