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All Journal ACTA VETERINARIA INDONESIANA Makara Journal of Science
Kiagus Dahlan
Department of Physics, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Veterinary

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Evaluasi Elektrokardiogram dan Radiografi Paru Domba Diimplan Scaffold Biphasic Calcium Phosphate/Alginate ., Gunanti; Dahlan, Kiagus; Angesti, Shavrillia; Afifa, Selma; Ramadian, Nuzula; Ravi, Sugganya; ., Soesatyoratih
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (852.71 KB) | DOI: 10.29244/avi.8.2.43-51

Abstract

Biomaterial merupakan suatu bahan yang digunakan untuk membantu persembuhan tulang sehingga mempunyai resiko terhadap organ jantung dan paru. Penanaman implan tulang dapat menyebabkan bone cement implantation syndrome yang dapat mempengaruhi aktivitas jantung. Degradasi implan pada tulang dapat menyebabkan efek pada paru. Penelitian ini bertujuan mengevaluasi perubahan pada hasil elektrokardiografi jantung dan radiografi paru domba (Ovis aries) yang diimplan scaffold biphasic calcium phosphate/alginate pada tulang tibia. Penelitian ini menggunakan 3 ekor domba jantan berusia 1.5 tahun dengan berat rata-rata 20 kg. Penanaman implan scaffold BCP/alginate dilakukan secara aseptis pada sepertiga proximal medial tulang tibia. Perekaman EKG pada domba dilakukan dalam keadaan sadar dan posisi berbaring lateral recumbency serta pemeriksaan radiografi dengan standar pandang left lateral recumbency dilakukan pada sebelum dan sesudah operasi, hari ke-7, 30, 60, dan 90 sesudah operasi. Parameter yang dievaluasi berupa amplitudo dan durasi gelombang P, durasi komplek QRS, durasi interval PR, amplitudo R, amplitudo T, durasi interval QT, durasi segmen ST, dan gambaran radiografi toraks. Data elektrokardiogram diolah menggunakan Microsoft Excel 2007 dan IBM Statistical Product and Service Solutions (SPSS). Data variabel dianalisis secara statistik dengan menggunakan metode One Way Analyse of Variant (ANOVA), kemudian dilanjutkan dengan uji Duncan pada selang kepercayaan 95%. Aktivitas elektrokardiografi jantung dan gambaran radiografi paru domba tidak mengalami perubahan setelah memperoleh penanaman implan scaffold BCP/alginate.
Adsorption of Waste Metal Cr(VI) with Composite Membranes (Chitosan-Silica Rice Husks) Zulti, Fifia; Dahlan, Kiagus; Sugita, Purwantiningsih
Makara Journal of Science Vol. 16, No. 3
Publisher : UI Scholars Hub

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Abstract

Chromium compounds are widely used in modern industry. Many of these compounds are dumped into the surrounding environment. Membrane technology is more efficient and effective than conventional methods for waste treatment. The research objective was to make a membrane separation process that can be applied to Cr(VI). Membranes were made from chitosan and silica rice husks. Variations of chitosan and silica rice husk used (g) are 2:1 (A1), 2:2 (A2), 3:1 (B1), and 3:2 (B2). The membrane was made by using an inverted phase technique. Results of SEM characterization of membranes showed that B2 has the largest pores at 2.58 μm. The FTIR characterization results indicate the presence of crosslinking between chitosan with silica rice husk with the appearance of Si-O adsorption band at wavelength 1122- 980/cm. A1 membrane, with the smallest pore size has the greatest rejection value towards Cr(VI) which is 70%. The result of this research showed that the composite membrane of silica rice husk was effective enough to adsorb metal Cr(VI) with an average adsorption capacity of 1665.85 mg/g.
APPLICATION OF HYDROXYAPATITE IN PROTEIN PURIFICATION Yusuf, Permai Sari Molyana; Dahlan, Kiagus; Witarto, Arief Budi
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

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Abstract

The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700o C of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700o C is less than 110o C. It indicates an increasing temperature of sintering will increase crystallinity and decrease carbonate content of hydroxapatite. This method gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of Protein Purification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purification by hydroxyapatite matrix, it showed that hydroxyapatite not only has DEAE matrix characteristic as anion exchange but also has kation exchange characteristic. These patterns proof that hydroxyapatite could be used in protein purification as matrix as cation an anion exchange. Additionally, apatite matrix is abiding and can be used repeatedly more than one hundred times without contamination.
Co-Authors Afifa, Selma Angesti, Shavrillia Arief Budi Witarto Gunanti . Purwantiningsih Sugita Raden Roro Soesatyoratih Ramadian, Nuzula Ravi, Sugganya Yusuf, Permai Sari Molyana Zulti, Fifia