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All Journal Distribusi Jurnal TAMBORA ASEAN Journal of Chemical Engineering Indonesian Journal of Cancer Makara Journal of Science Makara Journal of Technology
Arief Budi Witarto
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ANALISIS PENGELOLAAN PARIWISATA HALAL DI DESA TETE BATU KABUPATEN LOMBOK TIMUR NUSA TENGGARA BARAT Budi Witarto, Arief; Trishuta Pathiassana, Mega
Jurnal TAMBORA Vol. 4 No. 2A (2020): SPECIAL ISSUE INNOVATION MANAGEMENT
Publisher : Wakil Rektor 3, Direktorat Pengabdian kepada Masyarakat dan Publikasi, Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/jt.v4i2A.764

Abstract

The choice of NTB as one of the halal tourism destinations in Indonesialed to the passing of Perda No. 2 of 2016 concerning Halal Tourism in NTB.The implications of this regional regulation have been widely implemented. One of them is withmany emergence of halal tourism villages, especially on the island of Lombok. This researchaims to find out in detail the management of tourism in the Tete halal tourism villageBatu in East Lombok Regency which is one of the halal tourism destinationsNTB. This study uses a qualitative approach where the sample is selected bypurposive sampling. Data collection techniques using observation, interviews, anddocumentation. The data obtained were analyzed using three pathways, namely reduction,presentation of data, and drawing conclusions made at the time of data collectiontake place. The validity of the data was tested using the source triangulation technique. ResultThe research shows that the management of the Tete Batu halal tourism village includes:several aspects, namely planning, implementation, and supervision. 1) Deep planningthe process of tourism management in Tete Batu Village seems invisible, even more sofor tourism management which in fact has been implemented by the community alreadysince a long time ago, the concept of halal has been very thick with people's livesLombok. 2) The implementation of management is focused on the efforts made by the partiesmanagers in creating a halal brand image that is already well attached toin terms of supporting facilities for worship, providing halal food, lodging managementwith the concept of halal, and community participation in management is good enoughalthough there are still many deficiencies that must be addressed. 3) Supervisionimplemented in the form of monitoring and evaluation. The focus of supervision lies onimplementation of standardization of halal tourism and further evaluation of achievementsthe purpose of the brand image of halal tourism is to increase the number of visitstourists in NTB and more specifically in Tete Batu Village. Inner obstaclesmanagement of hala tourism in Tete Batu Village, among others, a lack of budget andHuman resources that support the development of halal tourism
ANALISIS PENGARUH PAPARAN FISIK PADA SAMPEL GIGI TERHADAP HASIL KUANTIFIKASI DNA FORENSIK MENGGUNAKAN METODE KIT PURIFIKASI DNA KOMERSIAL Arini, Septi; Budi Witarto, Arief; Betaria Aritonang, Setia
Jurnal TAMBORA Vol. 5 No. 2 (2021): EDISI 13
Publisher : Wakil Rektor 3, Direktorat Pengabdian kepada Masyarakat dan Publikasi, Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/jt.v5i2.1126

Abstract

Physical exposure to biological samples has an enormous influence on the results of forensic DNA analysis. The lack of molecular research on the effect of physical exposure on dental samples is the reason for the need for further research. This study aims to determine the effect of physical exposure on dental samples on the results of forensic DNA quantification. The parameter used is the concentration value of isolated DNA obtained from real time PCR analysis. The use of real time PCR allows the detection and quantification of specific sequences of DNA samples at the same time to be analyzed. The dental samples used were obtained from different individuals. Teeth are used as identification media because teeth are the hardest part of the body and are chemically the most stable and most resistant to degradation and decomposition. The method in this study is to give three types of treatment to the tested samples in the form of sea water immersion, river water immersion and exposure to free air at room temperature with each treatment consisting of three test samples. All samples were extracted using a Commercial DNA purification KIT with a reagent in the form of a Qiagen KIT (QIAamp® DNA Investigator) then a quantification process was carried out to see the value of the DNA concentration of each sample using real time PCR. The results of DNA quantification of dental samples from each treatment showed that the highest sample concentration value was based on the average of each treatment, namely samples with treatments exposed to free air at room temperature with a concentration value of 1.34 ng/µl, followed by samples soaked using river water with a concentration value of 0.15 ng/µl, while the sample with the lowest concentration is shown by a sample treated with seawater immersion with a concentration value of 0.10 ng/µl. Physical exposure in the form of exposure to free air, exposure to river water and exposure to sea water on dental samples, gave a not too significant effect on the results of DNA quantification produced.
INOVASI PENGELOLAAN DANA SIMPAN PINJAM KELOMPOK PEREMPUAN (SPP) PADA UNIT PENGELOLA KEGIATAN (UPK) DI KABUPATEN SUMBAWA Nudiawati Nudiawati; Farit M. Afendi; M. Budi Setiawan; Dedy Heriwibowo; Zulkieflimansyah Zulkieflimansyah; Arief Budi Witarto
Distribusi - Journal of Management and Business Vol. 7 No. 2 (2019): Distribusi, September 2019
Publisher : Fakultas Ekonomi dan Bisnis Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/distribusi.v7i2.85

Abstract

The purpose of this research is to know the level of refund Loan Save women's groups (SPP) at Sumbawa district and analyzing the strategy of Innovation management of the Fund Save Loan group of women (SPP) at Sumbawa district. The methods used in this research is quantitative methods that use the approach of the Ex post Facto on the five Subdistrict: Subdistricts of West Base, district, sub-district of Labuhan Badas Utan, Lopok sub district, and Sub-district Lape. The collection of data by the method of observation, kuisioner, documentation and interviews. The results of this study indicate that by doing the Innovation Fund management Save Loan group of women that used to be the level of kolektibilitas above 90% to 43% This means about 47% so an increase in refund the loan. So have significant results to increased kolektibilitas. The conclusion from this study that there are several causes of occurrence of nonperforming loans include: the existence of information that is not true that this is a fund that grants provided by the Government which does not need to be restored, failed harvest, the existence of communities that migrate, and used for other purposes that are urgent.
Enhanced Chlorella vulgaris Buitenzorg growth by Photon Flux Density Alteration in Serial Bubble Column Photobioreactors Anondho Wijanarko; Dianursanti Antonius Yudi Sendjaya; Misri Gozan; Roekmijati Widaningroem Soemantojo; Arief Budi Witarto; Kazuhiro Asami Kazuhisa Ohtaguchi
ASEAN Journal of Chemical Engineering Vol 7, No 1 & 2 (2007)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.265 KB) | DOI: 10.22146/ajche.50131

Abstract

Micro algae are photolitotrophs that perform oxygenic photosynthesis and capable of accumulating a large amount of CO2, using an inducible CO2 concentrating mechanism (CCM). These characteristics make the micro algae potentially useful for removal and utilization of CO2 emitted from industrial plant. Generally, the usage of photosynthetic microorganism in CO2 fixation and biomass production for the economically viable commodities have been increased and significantly improved as a solution for this problem. Using these facts and previous research results using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement of CO2 fixation and biomass production by Chlorella vulgaris Buitenzorg with photon flux density alteration along with an increasing of culture biomass during the cellular growth period, was implemented in this research. The photon flux density used in this alteration was the maximum light for Chlorella’s maximum growth rate ( I mmax,opt ). The cultivation of Chlorella vulgaris Buitenzorg in the Benneck basal medium operating conditions: T, 29oC; P, 1.0 atm; UG, 2.4m/h; CO2, 10%; using Philip Halogen Lamp 20W/12V/50Hz as the light source and three bubble column photobioreactors arranged in series order with each having a volume of 0.200dm3. Results had shown that the photon flux density alteration as a whole could increase around 60% the biomass production of Chlorella vulgaris and around 7% the CO2 fixation ability, compared to constant photon flux density outcomes. This experiment also showed that the noncompetitive inhibition of [HCO3-] as carbon source substrate is affected significantly during the cultivation in both of alteration and continuous photon flux density.
The mRNA Expression Profile of PD-1 and PD-L1 in Peripheral Blood of Colorectal Cancer Patients Ajoedi Ajoedi; Muhammad Al Azhar; Siti Nadliroh; Sri Hartini; Rizka Andalusia; Arief Budi Witarto
Indonesian Journal of Cancer Vol 13, No 3 (2019): September
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.293 KB) | DOI: 10.33371/ijoc.v13i3.670

Abstract

Background: Immunotherapy using immune checkpoint inhibitors has currently emerged as an effective treatment for a subset of colorectal cancer (CRC) patients. The roles of PD-1 and PD-L1 expression levels in peripheral blood to predict patient’s response to immune checkpoint inhibitors are not well established. Therefore, we analyzed PD-1 and PD-L1 mRNA expression levels of peripheral blood in Indonesian CRC patients and explored the association with the clinicopathological features.Methods: Peripheral blood of 25 CRC patients and 10 healthy individuals were collected in Dharmais Hospital-National Cancer Center from 2017 to 2018. PD-1 and PD-L1 mRNA expression levels were analyzed using real time PCR. The associations with clinicopathological variables were analyzed with fisher-exact test or chi square test.Results: PD-1 mRNA expression levels were significantly lower in CRC patients compared to healthy individuals (HI) (mean: 0.0015 ± 0.0013 and 0.017 ± 0.010 respectively, p < 0.001). Although PD-L1 mRNA expression levels were lower in CRC patients, the difference was not statistically significant (mean in CRC and HI: 0.021 ± 0.013 and 0.034 ± 0.028 respectively, p = 0.125). The expression of PD-L1 was higher in CRC females compared to males (p = 0.03). The expression levels of PD-L1 were not associated with different ages (p = 0.673), stages (p = 0.298), histological type of colorectal cancer (p=0.852), patient status (p = 1.000), and body mass index (p = 0.514).Conclusions: The mRNA expression levels of PD-1 and PD-L1 were lower in CRC patients compared to healthy controls. Expression of PD-L1 were correlated with sex, but not correlated with ages, stages, histological type of CRC, patient status, and body mass index.
Effect of Photoperiodicity on Co2 Fixation By Chlorella vulgaris Buitenzorg in Bubble Column Photobioreactor For Food Supplement Production Wijanarko, Anondho; Dianursanti, Dianursanti; Witarto, Arief Budi; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 8, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

To reduce the level of CO2 content in air, effort on converting CO2 to useful products is required. One of the alternatives includes CO2 fixation to produce biomass using Chlorella vulgaris Buitenzorg. Chlorella vulgaris Buitenzorg is applied for production of food supplement. Chlorella vulgaris Buitenzorg is also easy to handle due to its superior adaptation. Currently, Chlorella vulgaris Buitenzorg has been analyzed by some experts for its cellular composition, its ability to produce high quality biomass and the content of essential nutrition. A series of experiments was conducted by culturing Chlorella vulgaris Buitenzorg using Beneck medium in bubbling column photobioreactor. The main variation in this experiment was photoperiodicity, where growth of Chlorella vulgaris Buitenzorg was examined during photoperiodicity condition. The difference between CO2 gas concentration of inlet and outlet of the reactor during operational period, was compared to the same experiment under continuous illumination. Under photoperiodicity of 8 and 9 h/d, the culture cell densities (N) were approximately 40 % higher than under continuous illumination. Final biomass density of Chlorella vulgaris Buitenzorg at 9 h/d illumination was 1.43 g/dm3, around 46% higher than under continuous illumination. Specific carbon dioxide transfer rate (qCO2) in photoperiodicity was 50-80% higher than under continuous illumination. These experiments showed that photoperiodicity affects the growth of Chlorella vulgaris Buitenzorg The specific growth rate (μ) by photoperiodicity was higher than that by continuous ilumination while the growth period was two times longer. Based on the experiments, it can be concluded that photoperiodicity might save light energy consumption. The prediction of kinetic model under continuous illumination as well as under photoperiodicity illumination showed that Haldane model became the fitted kinetic model.
APPLICATION OF HYDROXYAPATITE IN PROTEIN PURIFICATION Yusuf, Permai Sari Molyana; Dahlan, Kiagus; Witarto, Arief Budi
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

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Abstract

The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700o C of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700o C is less than 110o C. It indicates an increasing temperature of sintering will increase crystallinity and decrease carbonate content of hydroxapatite. This method gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of Protein Purification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purification by hydroxyapatite matrix, it showed that hydroxyapatite not only has DEAE matrix characteristic as anion exchange but also has kation exchange characteristic. These patterns proof that hydroxyapatite could be used in protein purification as matrix as cation an anion exchange. Additionally, apatite matrix is abiding and can be used repeatedly more than one hundred times without contamination.
Co-Authors Ajoedi Ajoedi Anondho Wijanarko Anondho Wijanarko Arini, Septi Betaria Aritonang, Setia Dedy Heriwibowo Dianursanti Farit M. Afendi Febriana Catur Iswanti Kazuhiro Asami Kazuhisa Ohtaguchi Kiagus Dahlan Kiagus Dahlan M. Budi Setiawan Misri Gozan Mohamad Sadikin Muhammad Al Azhar Nudiawati Nudiawati Permai Sari Molyana Yusuf Rizka Andalusia Roekmijati Widaningroem Soemantojo Samsuridjal Djauzi Siti Nadliroh Soemantojo, Roekmijati Widaningroem Sri Hartini Tomohiko Yamazaki Trishuta Pathiassana, Mega Yusuf, Permai Sari Molyana Zulkieflimansyah Zulkieflimansyah