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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
E. Damayanti
BPPTK LIPI
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Effectivity of probiotic, micromineral enriched yeast and their combination with Azadirachta indica leaves containing tannin on fermentability and digestibility of Pennisetum hybrid Sofyan, Ahmad; Sakti, A. A.; Karimy, M. F.; Julendra, H.; Istiqomah, L.; Herdian, H.; Damayanti, E.; Suryani, A. E.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.217 KB) | DOI: 10.14334/jitv.v20i2.1164

Abstract

Organic additive for animal had been explored to replace antibiotic growth promoter. Probiotic from lactic acid bacteria was widely used to support the microbial balances in digestive tract, while organic mineral was added into diets to improve bioavailability for preventing mineral deficiency disorders. This experiment was aimed to assess probiotic (Pediococcus acidilactici RS2) and micromineral enriched yeast (MEY) combined with tannin from neem (Azadirachta indica) leaves containing tannin on king grass (P. hybrid) fermentability using in vitro gas production technique. Treatments consisted of P0 (control/forage without additive), P1 (P0+MEY); P2 (P0+MEY+crude tannin); P3 (P0+Probiotic); P4 (P0+Probiotic+MEY), and P5 (P0+Probiotic+MEY+crude tannin). The study was arranged in a completely randomized design (CRD) with three replications in each treatment. Probiotic, MEY or tannin supplementation significantly increased (P<0.05) gas production without affecting volatile fatty acid, protozoa numbers, methane production and in vitro digestibility of forage. The highest cumulative gas production was found in forage treated by P4 followed by P5, P1, P5, P2, P3 and control. Kinetic of gas production was significantly affected by treatments after 8 h incubation. Although the treatments were only significantly affected gas production kinetic (b, c and total gas), the hierarchical cluster analysis indicated that some parameters consisted of acetate, propionate, in vitro digestibility, protozoa numbers, and methane production were closely correlated to the gas production kinetic parameters. It was concluded that either organic mineral supplementation or its combination with probiotic, and probiotic+tannin improved fermentabilty of forage without negative effect on in vitro digestibility. Key Words: Fermentability, In Vitro Digestibility, Organic Mineral, Probiotic, Tannin
Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck Istiqomah, L.; Anwar, M.; Anggraeni, A.S.; Damayanti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.149-158

Abstract

This study had a purpose of obtaining potential indigenous yeasts for assimilating cholesterol and assessed the in vitro activity of Bile Salt Hydrolase (BSH) using Chloramphenicol Yeast Glucose (CYG) media supplemented CaCl2 and Taurodeoxycholic Acid (TDCA). Yeasts were collected from the gastrointestinal tract of Indonesian native chicken (Gallus javanicus), Javanese duck (Anas javanicus), and Muscovy duck (Anas moschata). The BSH assay was performed to determine secretion of BSH from yeast strain to conjugate bile salts into cholic acid-free by measuring precipitation zone in a specific medium. The quantitative measurement to assimilate cholesterol in yeast using CYG broth contained soluble cholesterol (500 ppm) and incubated at 30°C for 72 hours. Microplate reader used to analyze cholesterol content in the supernatant. Data were analyzed using Analysis of Variance (ANOVA) with one way completely randomized. Precipitation zone found among isolates did not significantly different (P>0.05). Isolate B-18 from Javanese duck performed the highest percentage of assimilating cholesterol with the value of 51.83% and identified as S. cerevisiae. This isolate was closely related to S. cerevisiae mt 21s (accession number X00149.1)based on phylogenetic tree analysis. It could be concluded that S. cerevisiae B-18was potential for assimilating cholesterol in vitro.
Cellulolytic yeast from gastrointestinal tract of muscovy duck (Anas moscata) as probiotic candidate Anggraeni, A.S.; Istiqomah, L.; Damayanti, E.; Anwar, M.; Sakti, A.A.; Karimy, M.F.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.361-372

Abstract

Cellulose is one of limiting factors in local poultry feed. Yeast exploration of local poultry gastrointestinal tract and its utilization as a probiotic candidate has not been widely studying. A total of 112 isolates of yeast were obtained from the digestive tract of Muscovy Duck, Javanesse Duck and Indonesian Native Chicken in the small intestine, cecum, and colon. Yeast with cellulolytic activity was quantitatively selected using CYG agar + 1% CMC medium with an incubation time of 30 ° C for 48 hours under anaerobic conditions. A total of 107 isolates of yeasts have cellulolytic activity. M41 yeast isolates of Mentok cecum possess highest clear zones cellulolytic activity (0.64 cm). M41 yeast characterization includes molecular identification, growth ability testing in CMC and glucose combination medium, CMCase activity, and viability test of probiotic ability with time variation. The results showed that yeast M41 had 98% proximity with Saccharomyces cerevisiae based on 26S sRNA gene sequence analysis, optimum growth on medium containing 1% CMC and 0.1% glucose (b / v), CMCase activity of 42.98 U / mL, and has a viability of 91.91; 66.22; 42.00; 42.00; 1.83 and 42.13 (%) respectively on bile salts 2 hours and 3 hours, gastric juice 45 minutes and 2 hours, pH 2 and pH 3 for 90 minutes. Based on results of the study, it was concluded that S. cerevisiae M41 potentially as a probiotic candidate for poultry with cellulolytic activity.
AMINO ACID PROFILE OF EARTHWORM AND EARTHWORM MEAL (Lumbricus rubellus) FOR ANIMAL FEEDSTUFF Istiqomah, L.; Sofyan, A.; Damayanti, E.; Julendra, H.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.253-257

Abstract

Earthworm meal (Lumbricus rubellus) has become one of natural material that could be used asfeed additive. Powdering method of earthworm was done by using formic acid addition. The study wascarried out (1) to evaluate the essential amino acid profile of earthworm and earthworm meal, (2) tocalculate the value of essential amino acid index (EAAI) of both materials. A modified EAAI equationwas developed from the essential amino acid profile of earthworm and earthworm meal. The resultshowed that essential amino acid of earthworm was dominated by histidine (0.63% of dry matter basis),meanwhile the earthworm meal was dominated by isoleucine (1.98% of dry matter basis). The nonessential amino acid of earthworm and earthworm meal was dominated by glutamic acid (1.52% and3.60% of dry matter basis respectively). The value of essential amino acid index obtained fromearthworm meal was higher (58.67%) than those from earthworm (21.23%). It is concluded thatpowdering method of earthworm by using formic acid addition had higher amino acid balance thanearthworm.
LACTIC ACID BACTERIAL SCREENING FROM GASTROINTESTINAL DIGESTIVE TRACT OF NATIVE AND BROILER CHICKEN FOR PROBIOTIC CANDIDATE PURPOSES Damayanti, E.; Herdian, H.; Angwar, M.; Febrisiantosa, A.; Istiqomah, L.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.168-175

Abstract

The aim of his research was to obtain lactic acid bacteria (LAB) from gastrointestinal digestivetract (GIT) of chickens for probiotic candidate purposes. LAB was isolated from GIT of broiler andnative chickens on selective medium (MRS+0.2% CaCO3). Screening method based on microbiologicaland biochemical characteristics, antibacterial properties, growth on various temperature, aeration, andagitation, antibiotic sensitivity, and viability on acid pH, gastric juice and bile salt. Thirty nine of LABisolates was selected from native chicken and 18 isolates from broiler chicken. The selected LABinhibited Escherichia coli FNCC 0091 growth and grown on 30, 39 and 45oC of temperature, aerobic,anaerobic and agitation conditions. Biochemical identification using API 50 CHL kit revealed that I72from native chicken ileum as Lactobacillus salivarius and Db9 from broiler chicken duodenum asPediococcus pentosaceus. All LAB were resistant to Erythromicin, Penicillin G and Streptomycin astested antibiotics. Both of them have non significantly different of viability on acid pH (1, 2 and 3),gastric juice pH 2 and bile salt which were 91.78% for L. salivarius I72 and 94.48% for P. pentosaceusDb9 (P<0.05). Based on characteristics, both the selected LAB have potentiality as chicken probioticcandidates.
Co-Authors A. A. Sakti, A. A. A. E. Suryani, A. E. A. Febrisiantosa A. Sofyan Anggraeni, A.S. Anwar, M. H. Herdian H. Julendra Karimy, M.F. L. Istiqomah M. Angwar M. F. Karimy, M. F. Mudinillah, Adam Sakti, A.A.