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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
A. Febrisiantosa
Technical Implementation Unit for Development of Chemical Engineering Processes Indonesian-Institute of Sciences (UPT. BPPTK - LIPI) Jl. Jogja – Wonosari Km. 31, Gading, Playen, Gunungkidul, D.I.Yogyakarta 55861
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Effect of Morinda citrifolia leaf as saponin sources on fermentation characteristic, protozoa defaunated, gas and methane production of ruminal fluid in vitro Herdian, Hendra; Istiqomah, L.; Febrisiantosa, A.; Setiabudi, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.186 KB) | DOI: 10.14334/jitv.v16i2.639

Abstract

Many studies have reported that the Morinda citrifolia (pace plant) was a useful material for human health. However the exploration of this plant on rumen fermentation is still needed. Therefore, a research was done to study the effect of M. citrifolia leaf on fermentation characteristics of rumen fluid consisted of protozoa defaunated process, VFA composition, NH3 content, rumen microbial protein content, gas and methane production using in vitro techniques. Rumen fluid obtained from two fistulated Ongole crossbreed cattle fed with forage and concentrate feed ration (70 : 30). The fluid was incubated at 39ºC for 48 hours. The treatment on the rumen fluid consisted of control treatment: 100% (200 mg DM) kolonjono forage substrate (Penisetum purpureum) and M. citrifolia treatments: kolonjono forage plus M. citrifolia (equivalent saponin) 3; 6; 9; and 12 mg DM, respectively. The treatment of M. citrifolia leaf addition showed declined patterns in the number of protozoa population (P < 0.05) compared to control. There was no effect of M. citrifolia treatment on VFA composition and NH3 content (P > 0.05). Microbial protein content in rumen fluid increased (P < 0.05) by M. citrifolia treatments compared to control. Effect of M. citrifolia treatments on the gas production was not significantly different (P > 0.05) compared to control, while M. citrifolia treatments reduced the methane gas production of (P < 0.05) compared to control. It was concluded that M. citrifolia leaf has potential as a limiting agent of protozoa population and methane gas production in rumen. Key words: M. citrifolia, Defaunated, Saponin, In Vitro
LACTIC ACID BACTERIAL SCREENING FROM GASTROINTESTINAL DIGESTIVE TRACT OF NATIVE AND BROILER CHICKEN FOR PROBIOTIC CANDIDATE PURPOSES Damayanti, E.; Herdian, H.; Angwar, M.; Febrisiantosa, A.; Istiqomah, L.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.168-175

Abstract

The aim of his research was to obtain lactic acid bacteria (LAB) from gastrointestinal digestivetract (GIT) of chickens for probiotic candidate purposes. LAB was isolated from GIT of broiler andnative chickens on selective medium (MRS+0.2% CaCO3). Screening method based on microbiologicaland biochemical characteristics, antibacterial properties, growth on various temperature, aeration, andagitation, antibiotic sensitivity, and viability on acid pH, gastric juice and bile salt. Thirty nine of LABisolates was selected from native chicken and 18 isolates from broiler chicken. The selected LABinhibited Escherichia coli FNCC 0091 growth and grown on 30, 39 and 45oC of temperature, aerobic,anaerobic and agitation conditions. Biochemical identification using API 50 CHL kit revealed that I72from native chicken ileum as Lactobacillus salivarius and Db9 from broiler chicken duodenum asPediococcus pentosaceus. All LAB were resistant to Erythromicin, Penicillin G and Streptomycin astested antibiotics. Both of them have non significantly different of viability on acid pH (1, 2 and 3),gastric juice pH 2 and bile salt which were 91.78% for L. salivarius I72 and 94.48% for P. pentosaceusDb9 (P<0.05). Based on characteristics, both the selected LAB have potentiality as chicken probioticcandidates.
Co-Authors D. Setiabudi E. Damayanti H. Herdian Hendra Herdian L. Istiqomah M. Angwar