Garuda - Garba Rujukan Digital

Vycke Yunivita

Department of Biomedical Sciences Faculty of Medicine Universitas Padjadjaran

Author-ID : 2499059

Medicine & Pharmacology

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Articles

Inhibitory Effect of Neem Leaves on Glucose Transport Lia Safitrie Johansyah; Vycke Yunivita; Anna Martiana; Augusta Y. L. Arifin
Althea Medical Journal Vol 5, No 4 (2018)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Neem leaf is known as a diabetic herbal treatment in India, however, its antihyperglycemic effect and the mechanism is still unknown. Quercetin is proven to inhibit glucose transporter in the small intestine, and this active substance is present in Neem leaves. The study was conducted to explore the inhibitory effect of Neem leaves infusion on glucose transport and whether the effect was reversible or irreversible.Methods: This study was conducted in the Biochemistry Laboratory Faculty of Medicine Universitas Padjadjaran in the period of October to November 2012 by using in situ perfusion method. Five male Wistar rats were given three treatments; glucose as initial control, glucose with Neem infusion, and glucose again as last control. The samples of perfusion solution were taken for every treatment and were measured using spectrophotometry method.Results: There was a reduction in glucose absorption (15.9%) between the first (47.28+17.57 mg/dL) and the second treatment (39.75+14.85 mg/dL). The transported glucose level in the third treatment was further reduced after the first treatment (37.15+13.15 mg/dL).Conclusions: Neem leaves infusion has inhibitory effect on glucose transport that irreversibly reduced further. Further study is needed with a larger sample size to confirm this phenomenon.

Annona Muricata L Leaf Infusion Effect on Glucose Absorption in the Intestinal Cells Membrane of Wistar Rats Model Vycke Yunivita; Putra Haqiqie Adnantama Lubis; Augusta Y. L. Arifin
Althea Medical Journal Vol 6, No 2 (2019)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Diabetes mellitus (DM) is a chronic disease that disrupts the homeostasis of glucose. DM is the second of most causes of deaths in urban areas, as well as the sixth in rural areas. Therefore, herbal medicine is constantly developed. Soursop leaves have been long known to have good effects in lowering glucose levels in our blood. This study explored the soursop leaves infusion on glucose absorption in the intestinal cell membrane of the Wistar Rats Model. Methods: This was an exploratory study performed in October 2012 on five male rats Wistar, aged 3-4 months, weighing about 150-300 grams. Using the in situ perfusion method, each rat was given the following; 3 X 10-3 M glucose solution for one hour; 3 X 10-3 M glucose solution with 1 mL of infusion of soursop leaves (Annona muricata L) for one hour; 3 X 10-3 M glucose solution for the last hour. The glucose absorption levels were measured using spectrophotometry method. The glucose level between treatments was analyzed using a paired sample t-test.Results: The concentration of glucose absorption was decreased, the first treatment was 24.42+14.29 mg/dL, the second treatment was 18.63+10.37 mg/dL, and the third treatment was 18.24+8.02mg/dL), however, the decrease of glucose absorption was statistically not significant.Conclusions: There is a slightly decreased of the glucose absorption in the intestinal cell membranes of the Wistar Rats Model after infusion of soursop leaves. Further study is needed to explore the role of soursop leaves infusion in glucose absorption.

Effect of Carica Papaya L Leaf Infusion on Transporting Glucose across Small Intestine Cell Membrane of Wistar Rats Model Srisathis Renganathan; Vycke Yunivita; Anna Martiana S; Nida Suraya
Althea Medical Journal Vol 6, No 1 (2019)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Carica papaya L leaf is one of the common types of plant that has been used as treatment for diabetes mellitus (DM). The leaf possibly contain quercetin, which may inhibit glucose transport through cell membrane of small intestine. This study was conducted to determine the effect of Carica papaya L leaf infusion on glucose transport in Wistar rat model.Methods: A one-group pretest-posttest explorative study was designed using perfusion method. Nine male rats were fasted for 18-24 hours before dissected. A 25 mL glucose solution was given as early control, before Carica papaya L leaf infusion treatment A 25 mL glucose solution was later given again after Carica papaya L leaf infusion as late control. Artificial perfusion equipment had been used to maintain the back and forth flow of glucose in the small intestine. Sample was taken every 15 minutes for one hour for each treatment.Results: Carica papaya L leaf infusion did not decrease the concentration of glucose absorption significantly compared to early control. The mean absorption of glucose in early control was 85.39+4.42 mg/dl and during treatment was 84.51+4.20 mg/dL.Conclusions: Carica papaya L leaf infusion does not have significant inhibiting effect on glucose transport across small intestine cell membrane. Further explorative study is required.

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