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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Caraka Tani - Agriculture Science Journal
Linayanti Darsana
Program Studi Agroteknologi, Jurusan Agronomi, Fakultas Pertanian, Universitas Sebelas Maret Jl. Ir. Sutami 36 A Kotak Pos 4, Surakarta 57101, Indonesia
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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STUDI KARAKTERISASI ANGGREK SECARA SITOLOGI DALAM RANGKA PELESTARIAN PLASMA NUTFAH Hartati, Sri; Darsana, Linayanti; Cahyono, Ongko
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 1 (2014): Articles in Press
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Abstract

Karakter sitologi anggrek  alam sangat penting dipelajari guna mendukung keberhasilan pemuliaan anggrek. Tujuan penelitian untuk mempelajari karakter sitologi anggrek ( jumlah, ukuran ) serta karyotipe kromosom dalam rangka pelestarian plasma nutfah. Tempat penelitian analisis sitologi dilakukan di Laboratorium Bioteknologi  Fakultas Pertanian UNS Surakarta. Bahan penelitian adalah bagian ujung akar dari  Paphiophedilum glaucophylum,, Coelogyne spesiosa Dendrobium crumentum, Dendrobium mutabile, , Bulbophyllum. blumei.dan  Bulbophyllum beflorum.  Metode yang digunakan dalam penelitian ini adalah metode squashing (pencet) meliputi: fiksasi : larutan Carnoy 2 (6 etanol : 3 kloroform : 1 asam asetat glasial 45%), hidrolisis (larutan HCl 1 N ) dan pewarnaan kromosom (larutan aceto-orcein 2%). Pengamatan meliputi (jumlah, ukuran, bentuk) kromosom dengan mikroskop cahaya.  Hasil penelitian ini menunjukkan :.1.Jumlah Kromosom Anggrek Paphiopedilum glaucophylum, Coelogyne speciosa, Dendrobium crumenantum, Dendrobium mutabile dan Bulbophyllum blume dan Bulbophyllum beflorum mempunyai jumlah kromosom yang sama yaitu 2n = 38, sedang Ukuran kromosom Paphiopedilum glaucophyllum  4.26 ± 0.14 sampai 9.73± 0.19, Coelogyne spesiosa 0.35 ± 0.03 sampai 6.30 ± 0.84, Dendrobium crumenantum 2.46 ± 0.20 sampai 5.33 ±  0.02 Dendrobium mutabile  2.56 ±  0.72 sampai 6.54±   0.73 , Bulbophyllum blume 2.76 ±  0.09 sampai 6.07 ±  0.43  dan Bulbophyllum beflorum1.04 ±  0.07 sampai 1.35 ±  0.16 . Pola karyotipe Paphiopedillum glaucophyllum 2n = 38 m, Dendrobium crumenantum 2n = 38, Bulbophyllum blumei 2n = 38 m , Bulbophyllum beflorum 2n = 38 m sedang Coelogyne spesiosa 2n = 37 m+ 1 ak dan Dendrobium mutabile 2n = 37 m+ 1 sm
Karakterisasi Anggrek Alam secara Morfologi dalam Rangka Pelestarian Plasma Nutfah Hartati, Sri; Darsana, Linayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.366 KB) | DOI: 10.24831/jai.v43i2.10419

Abstract

ABSTRACTMorphological characters of natural orchids (species) is an important key for successful breeding program of orchids. This study was aimed to determine the morphological diversity and to study the genetic relationship among natural orchids from Java. The characterization was conducted on 12 orchids collected from Central, East and West Java from April to September 2012. Twenty five characters including stem, leaves, flower, pseudobulb, and root type were evaluated and scored based on Guidance of Orchid Characterization. Cluster analysis was done using the NTSYSpc version 2.02i under UPGMA function of SimQual. The study showed that there was a diversity on morphology of stem, pseudo bulb, leaf, flower and root type. Dendrogram based on genetic similarity at 52% clustered the population into two groups. The first group consisted of Paphiopedilum purpurascens, P. javanicum, P. glaucophylum, Coelogyne flexuosa, C. spesiosa, Dendrobium mutabile. Bulbophyllum blumei and B. biflorum. The second group was Coelogyne tomentosa, C. trinervis, D. crumenatum, and B. flavescens LindlKeywords: cluster analysis, dendrogram, diversity, morphology
Co-Authors Ongko Cahyono Sri Hartati