LATIFAH KOSIM DARUSMAN
Biopharmaca Study Centre, Institute of Research and Community Services, Bogor Institute of Agriculture

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Inhibisi Lipase Pankreas secara In Vitro oleh Ekstrak Air dan Etanol Daun Asam Jawa (Tamarindus indica) dan Rimpang Kunci Pepet (Kaempferiae rotundae) Pradono, Dyah Iswantini; Darusman, Latifah Kosim; Susanti, Ai
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.355 KB) | DOI: 10.31258/jnat.13.2.146-154

Abstract

Tamarind and kunci pepet has been used traditionally as herbal medicine to reduce body weight or used asantiobesity. But the mechanism in vitro of this herbal in reducing body weight has not been known yet. Theobjective of this research is to evaluate these herbal as antiobesity by their water and ethanol extracts capabilityin inhibiting pancreatic lipase activity in vitro at pH 8, incubation time 45 minutes, and temperature 400C. Pancreaticlipase used in this research was human pancreatic lipase with concentration of 1.4 × 10-5 μg/μl and the substratewas sesame oil with concentration of 16.2 μg/μl. The water and ethanol extracts of tamarind leaves containedalkaloids, flavonoids, saponins, steroids, and tannins. Water extract of kunci pepet contained alkaloids, flavonoids,saponins, and tannins, while ethanol extract contained alkaloids, flavonoids, saponins, and triterpenoids. Theresults of tamarind leaves extracts showed that ethanol extract in concentration of 150 ppm had the highestinhibitory effect, with the value of 49.0%. Water extract of kunci pepet at concentration of 200 ppm had the highestinhibition, with the value of 65.1%. These values were higher than inhibitory effect of Xenical® 100 ppm as thepositive control, with the inhibition value of 10.6%.
Sansevieria trifasciata Properties as Lead(II) Ion Biosorbent Yuningsih, Lela Mukmilah; Batubara, Irmanida; Darusman, Latifah Kosim
Makara Journal of Science Vol. 18, No. 2
Publisher : UI Scholars Hub

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Abstract

Sansevieria trifasciata (also called snake plant or mother in law’s tongue) is predicted to act as a heavy metal biosorbent. S. trifasciata was optimized as a biosorbent by using the response surface method with varying weights of S. trifasciata, pH of Pb (II) solutions, contact times, and temperatures. The ion concentration before and after biosorption was measured with an atomic absorption spectrophotometer (AAS). The optimum biosorption conditions were pH 7, 240 min contact time, and 1.5 g biosorbent with biosorption capacity of Pb (II) ions 0.725 mg/g. The biosorption isotherm analysis showed that the biosorption is consistent with the Freundlich isotherm model. The peak intensity of the FTIR spectrum of S. trifasciata after treatment with Pb (II) was around 2130/cm. The S. trifasciata micrograph showed a porous structure with non-uniform pore sizes. The biosorption of Pb (II) ions on powdered S. trifasciata leaves was found with the SEM EDX analysis. It is concluded from this research that S. trifasciata can be used as a biosorbent to remove Pb ion contamination.
Transformasi Nitrogen dalam Tanah Tergenang: Aplikasi Jerami Padi dan Kompos Jerami Padi Indriyati, Lilik Tri; Sabiham, Supiandi; Darusman, Latifah Kosim; Situmorang, Rykson; Sudarsono, .; Sisworo, Widjang Herry
JOURNAL OF TROPICAL SOILS Vol. 13 No. 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.189-197

Abstract

The use of organic materials aimed at reducing the dependence on inputs such as chemical fertilizers can contribute to sustainability and improving the low N fertilizer efficiency of rice plants in paddy soils.  Therefore, better understanding of N transformation in flooded soils, particularly the microbial transformation of N-organic amendments to plant-available N and gaseous N forms is needed for most efficient use of soil and organic materials N, for determining the potential of denitrification and for aiding in the selection of N management practices for sustainable agriculture.  The experiments were conducted in the laboratory and glasshouse of Soil Department, Faculty of Agriculture, Bogor Agricultural University.  An incubation experiment was conducted in the laboratory at room temperature during 120 d to analyze the mineralization-immobilization patterns in flooded soils amended with rice straw, rice straw composts 4 mo and 8 mo and their combinations with urea.  The first time of soil flooding, mineral N (N-NH4+ and N-NO3-) concentrations in all of the amended soils sharply decreased and those gradually increase since 7 d of incubation.  It might be due to the reduction of N-NO3- to N2O and N2, and immobilization.  This data was consistent with the data of N2O emission derived from pot experiment.  The high emission of N2O was observed at the first time of soil flooding of the amended soils, and the soils added rice straw showed the largest N2O emission than the other treatments. It might be due to the change of soil condition from aerobic to anaerobic condition, and the higher decomposable C as energy source for denitrifyer contained in rice straw.  Regardless the organic materials added to soils, the longer anaerobic condition, nitrification sharply decreased, so that nitrate availability limits denitrifications.decreased, so that nitrate availability limits denitrifications.