Garuda - Garba Rujukan Digital

Rifqoh Rifqoh

Politeknik Kesehatan Kementerian Kesehatan Banjarmasin

Author-ID : 2505427

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

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Comparison of Clinical Assessment and Adhesive-Tape Laboratory Microscopic of Sarcoptes scabiei for Scabies Diagnostic Rifqoh Rifqoh; Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Scabies infestation caused by Sarcoptes scabiei mite that infected the skin by making the tunnel burrow. Diagnosis of scabies infestation commonly relies on clinical assessment (CA). However, some scabies symptoms are similar to other diseases. Diagnosis of confirmed scabies can be made by identifying egg or Sarcoptes scabiei mite or scybala through skin scraping laboratory microscopic examination, which was the adhesive tape microscopic (AT) a noninvasive alternative. We aimed to compare the scabies diagnostic accuracy of CA and AT in Islamic Boarding School. This study was an analytical observational cross-sectional study. The population was 94 students of Islamic Boarding School, determining the sample base on purposive sampling technique. There were 34 samples with presumptive scabies analyzed with CA by two clinicians and AT by two trained laboratory technicians. The result showed that the number of patients who positive scabies by CA was 14 (41.18 percent) and 31 (91.18 percent) by AT. Sensitivity was 41.93 percent for CA and 92.85 percent for AT, and the difference was significantly based on Cohen's kappa (ⱪ=0.024). The number of positive cases with both techniques was 13 (38.24 percent). The number of patients positive with only CA was 1 (2.93 percent), and only AT was 18 (22.9 percent). The accuracy of CA was only 44,11 percent. The study concluded that CA is low accuracy than AT. The adhesive tape test is easy, needs no expensive equipment. It is recommended that adhesive tape test for screening purposes. The appropriate comprehensive of both methods for scabies diagnostic is highly recommended.

Development of Modified Kato Katz Kit as Laboratory Scale Prototype for Early Detection of Soil-Transmitted Helminths (STHs) Infection Rifqoh Rifqoh; Yayuk Kustiningsih; Jujuk Anton Cahyono
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Early detection of Soil-Transmitted Helminths (STHs) infection in laboratory medical facilities is one of the government's efforts in its elimination. Government regulations recommend Kato-Katz's quantitative method forthe diagnosis of STHs infection. Kato-Katz's domestic product is not availableyet. This research aims to create a laboratory-scale modified Kato-Katz kit for early detection of STHsinfection by fecal egg counts (FECs) in estimating the number of eggs per gram of stool (EPG).An experimental study was conducted to create a resin template, spatulas, and 3M-adhesive tape as a modified Kato-Katz Kit. 120 Kato-Katz thick smear preparations were done from 30 samples for modified and standard Kato-Katz with duplication. The amount of stool for each was weighed. All Kato-Katz thick smear microscopic examination was done at least 60 minutes by identifying the STHs eggs and counting them.The result for both methods showed no differences in EPG for Trichuris trichiura eggs p-value=0.012 (p<0.05), meanwhile the differences for Ascaris lumbricoides eggs p-value=0.156, and Hookworm eggs p-value=0.102 (p>0.05). Under microscopic examination, some A.lumbricoides eggs morphology was not visible because the adhesive in modified Kato-Katz blurry it, and the Hookworm fragile eggshells made it disappear within less than 60 minutes.The study concluded no difference in EPG for T.trichiura eggs by FECs betweenmodified Kato-Katzas this study product with the standard. It is recommended to improve modified Kato-Katz in further study, especially the adhesive tape, before using it as a quantitative toolforearly detection of STHs infection.

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Journal : Medical Laboratory Technology Journal