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neni oktiyani
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Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus neni oktiyani; sunarti Sunarti; Prasetyastuti Prasetyastuti; Jujuk Anton Cahyono
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.684 KB) | DOI: 10.31964/mltj.v1i1.4

Abstract

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes
Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Nurlailah Nurlailah; Nurhayati Aslami Alma; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.806 KB) | DOI: 10.31964/mltj.v3i1.148

Abstract

Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.
Comparison of Clinical Assessment and Adhesive-Tape Laboratory Microscopic of Sarcoptes scabiei for Scabies Diagnostic Rifqoh Rifqoh; Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.653 KB) | DOI: 10.31964/mltj.v7i1.379

Abstract

Scabies infestation caused by Sarcoptes scabiei mite that infected the skin by making the tunnel burrow. Diagnosis of scabies infestation commonly relies on clinical assessment (CA). However, some scabies symptoms are similar to other diseases. Diagnosis of confirmed scabies can be made by identifying egg or Sarcoptes scabiei mite or scybala through skin scraping laboratory microscopic examination, which was the adhesive tape microscopic (AT) a noninvasive alternative. We aimed to compare the scabies diagnostic accuracy of CA and AT in Islamic Boarding School. This study was an analytical observational cross-sectional study. The population was 94 students of Islamic Boarding School, determining the sample base on purposive sampling technique. There were 34 samples with presumptive scabies analyzed with CA by two clinicians and AT by two trained laboratory technicians. The result showed that the number of patients who positive scabies by CA was 14 (41.18 percent) and 31 (91.18 percent) by AT. Sensitivity was 41.93 percent for CA and 92.85 percent for AT, and the difference was significantly based on Cohen's kappa (ⱪ=0.024). The number of positive cases with both techniques was 13 (38.24 percent). The number of patients positive with only CA was 1 (2.93 percent), and only AT was 18 (22.9 percent). The accuracy of CA was only 44,11 percent. The study concluded that CA is low accuracy than AT. The adhesive tape test is easy, needs no expensive equipment. It is recommended that adhesive tape test for screening purposes. The appropriate comprehensive of both methods for scabies diagnostic is highly recommended.
Akurasi Hitung Jumlah Eritrosit Metode Manual dan Metode Otomatis Neni Oktiyani; fahriyan fahriyan; Akhmad Muhlisin
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.051 KB) | DOI: 10.31964/mltj.v3i2.166

Abstract

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.
PERBANDINGAN KADAR ALANINE AMINOTRANSFERASE BERDASARKAN FREKUENSI TRANSFUSI DARAH PASIEN TALASEMIA β MAYOR DI RSD BANJARBARU Rahmawati; Neni Oktiyani; Yayuk Kustiningsih; Jujuk Anton Cahyono
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.95

Abstract

Talasemia merupakan kelainan darah bawaan yang diakibatkan adanya gangguan sintesis hemoglobin di dalam sel darah merah yang mengharuskan mereka untuk mendapatkan transfusi secara rutin. Transfusi yang berulang-ulang dapat menyebabkan penumpukan zat besi dalam tubuh terutama organ hati, sehingga menyebabkan kadar Alanine Aminotransferase meningkat. Penelitian bertujuan mengetahui perbandingan kadar ALT berdasarkan frekuensi transfusi darah pada penderita talasemia β mayor di RSD Banjarbaru. Penelitian ini menggunakan jenis penelitian survei analitik. Teknik pengambilan menggunakan teknik accidental sampling. Hasil penelitian sebanyak 9 orang (30%) subjek penelitian memiliki kadar ALT diatas normal. Hasil pemeriksaan kadar ALT transfusi darah 2 minggu sekali kisaran 15-70 U/L, 3 minggu sekali kisaran 30-51 U/L, dan 4 minggu sekali kisaran 12-41 U/L. berdasarkan Uji One Way Anova didapatkan signifikansi (p = 0,044) yang berarti terdapat perbedaan bermakna paling tidak antar 2 kelompok. Kesimpulan ada perbedaan yang bermakna antara kadar ALT dengan frekuensi transfusi darah.
PERBANDINGAN KADAR HEMOGLOBIN ANTARA ANAK SD DI DATARAN RENDAH DAN TINGGI WILAYAH KERJA PUSKESMAS DIRGAHAYU KOTABARU TAHUN 2024 Rahmatus Sholehah; Neni Oktiyani; Ahmad Muhlisin; Wahdah Norsiah
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.218

Abstract

Hemoglobin ialah unsur utama dalam sel darah merah yang tergolong sebagai protein terkonjugasi, berfungsi penting dalam proses pengangkutan oksigen serta karbon dioksida dalam tubuh. Hasil pemeriksaan terhadap sejumlah parameter darah seperti eritrosit, hemoglobin, dan hematokrit memperlihatkan adanya perbedaan yang mencolok antara individu yang tinggal di daerah dataran tinggi dengan mereka yang menetap di daerah dataran rendah. Kondisi kadar oksigen yang menurun seiring dengan peningkatan ketinggian menyebabkan tubuh beradaptasi dengan meningkatkan produksi sel darah merah demi mencukupi kebutuhan oksigen. Semakin tinggi lokasi tempat tinggal, semakin tinggi pula jumlah eritrosit yang diproduksi. Oleh karena itu, jumlah eritrosit memiliki kaitan erat dengan kadar hemoglobin dan hematokrit. Penelitian ini bertujuan guna mengetahui apakah ada perbedaan kadar hemoglobin antara siswa sekolah dasar yang berdomisili di dataran tinggi dan dataran rendah di wilayah kerja Puskesmas Dirgahayu, Kabupaten Kotabaru. Penelitian ini menggunakan desain observasional analitik dengan pendekatan cross sectional dan bersifat komparatif. Pemeriksaan dilakukan secara otomatis menggunakan alat hematology analyzer terhadap sampel darah vena dari 15 siswa di dataran rendah dan 15 siswa di dataran tinggi. Hasil analisis menggunakan uji Mann Whitney U menunjukkan nilai signifikansi sebesar 0,003, yang berarti ada perbedaan yang signifikan antara kedua kelompok. Rata-rata kadar hemoglobin pada siswa di dataran rendah tercatat sebesar 11,6 g/dl, sedangkan pada siswa di dataran tinggi mencapai 12,6 g/dl. Penelitian selanjutnya disarankan guna dilakukan pada wilayah dengan ketinggian 725 mdpl dengan melibatkan subjek remaja maupun orang dewasa.
PERBEDAAN KUALITAS PEWARNAAN SEDIAAN SITOLOGI MENGGUNAKAN BUNGA TELANG (Clitoria ternatea L) SEBAGAI ALTERNATIF PENGGANTI METHYLENE BLUE PADA METODE DIFF QUICK Rizky Arsyanti; Neni Oktiyani; Aima Insana; Ratih Dewi Dwiyanti
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.232

Abstract

Pewarnaan sediaan sitologi dilakukan untuk memperjelas morfologi sel agar dapat diamati secara mikroskopis, khususnya pada bagian inti sel dan sitoplasma. Pewarnaan Diff Quick merupakan metode yang umum digunakan karena cepat dan memiliki kontras warna yang baik, tetapi salah satu komponennya, methylene blue, bersifat toksik dan tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui perbedaan kualitas pewarnaan sediaan sitologi menggunakan larutan bunga telang (Clitoria ternatea L) sebagai alternatif alami pengganti methylene blue dengan pewarnaan Diff Quick. Jenis penelitian True Experiment dengan rancangan Post Test Only with Control Group Design, terdiri dari 4 perlakuan dengan 6 kali pengulangan menggunakan 1 sampel cairan pleura yang diwarnai dengan larutan bunga telang pada konsentrasi 20%, 25%, dan 30%, serta dibandingkan dengan pewarnaan Diff Quick standar sebagai kontrol. Hasil pemeriksaan mikroskopik larutan bunga telang konsentrasi 20% menunjukkan kualitas tidak baik, 25% menunjukkan kualitas cukup baik dan 30% menunjukkan kualitas pewarnaan yang paling mendekati pewarnaan standar dengan kategori “sangat baik.” Uji Friedman menunjukkan perbedaan yang signifikan antar kelompok perlakuan (p=0,001), dan uji Wilcoxon menunjukkan tidak terdapat perbedaan signifikan antara larutan bunga telang 30% dengan kontrol (p=0,063). Kesimpulannya, larutan bunga telang konsentrasi 30% memiliki potensi sebagai pewarna alami alternatif methylene blue dalam Metode Diff Quick. Disarankan untuk dilakukan penelitian lebih lanjut dengan menaikkan konsentrasi larutan pewarna bunga telang atau menaikkan pH larutan pewarna bunga telang (Clitoria ternatea L).
Co-Authors Ahmad Muhlisin Aima Insana Akhmad Muhlisin fahriyan fahriyan Jujuk Anton Cahyono Jujuk Anton Cahyono Nurhayati Aslami Alma Nurlailah Nurlailah Prasetyastuti Prasetyastuti Rahmatus Sholehah Rahmawati Rahmawati Ratih Dewi Dwiyanti Rifqoh Rifqoh Rizky Arsyanti Sunarti Sunarti wahdah norsiah Yayuk Kustiningsih