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Kadar Logam Timbal (Pb) dalam Darah Penjual Klepon Yayuk Kustiningsih; Noni Fitriyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

A kelepon seller is one of the groups susceptible to metal pollution from motor vehicle emissions from the tetraethyl-Pb and tetramethyl-Pb combustion products that are always added to motor vehicle fuel. Lead metal has a negative impact on the environment including human health. The first effect of chronic Pb poisoning before reaching the target organ is the presence of hemoglobin synthesis disorder so that the hemoglobin level decreases.The effect of lead poisoning in the blood can occur if the Pb content is more 70 ug/dl or 0.7 ppm which will lead to anemia. This study aims to determine a level of lead metal in the blood of kelepon seller that exceeds the threshold. Descriptive survey research method with cross-sectional design on the sample from 15 respondents. The lead metal (Pb) in the blood is measured using an Atomic Absorption Spectrophotometer (SSA) device. The result of the research shows that there are 13 respondents (86,7%) whose Pb metal content exceeds the blood lead threshold that is 20 ug / dL or 0,20 ppm. The highest level of lead in the blood of the respondents was 0.73 ppm and the lowest was 0.10 ppm. Two respondents with lead in blood less than 0.20 ppm are known to always use personal protective equipment while working, even though the working time is 6-10 hours/day. It is advisable to conduct further research by examining Hb levels and abnormalities of erythrocytes in the blood of the kelepon seller and conducting similar research

Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik Yayuk Kustiningsih; Nastiti Megawati; Jasmadi Joko Kartiko; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Pre-analysis phase is the first step that determines the quality of the examination results, in this case, is the preparation of glucose work reagent. Glucose is one of the clinical examination clinical parameters that are often done in the laboratory. This glucose examination uses enzymatic methods. Enzyme activity is influenced by several factors, namely temperature, pH, substrate level, enzyme level, and inhibitor. Glucose examination performed at a temperature of 20-25oC or 37oC, but there are still laboratories in the field that is less attention to the initial temperature conditions of this reagent. This study aims to determine the effect of initial temperature variations of reagents on blood glucose levels of enzymatic methods. The research used Quasi Experimental research method with Posttest-Only Control Group Design design. The sample of this research used serum of a respondent who had no history of Diabetes Mellitus disease with 4 replication at each treatment. The results of the study with the initial temperature of 10oC, 13oC, 16oC, 19oC, 22oC and 25oC (control) respectively of 62.71 mg / dL; 65,74 mg / dL; 72.45 mg / dL; 76.91 mg / dL; 89.12 mg / dL and 97.19 mg / dL, the results of this study showed an increase in glucose levels in each treatment. Based on the results of linear regression analysis obtained a significance value of 0.000 with α = 0.05, which means Ho is rejected, so it can be concluded that there is an influence of initial temperature variation of reagent on blood glucose level. For the next researcher can do research about the effect of the initial temperature of reagent 22-38 oC on blood glucose enzymatic method. And the influence of incubation time on blood glucose level of enzymatic method.

Development of Modified Kato Katz Kit as Laboratory Scale Prototype for Early Detection of Soil-Transmitted Helminths (STHs) Infection Rifqoh Rifqoh; Yayuk Kustiningsih; Jujuk Anton Cahyono
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Early detection of Soil-Transmitted Helminths (STHs) infection in laboratory medical facilities is one of the government's efforts in its elimination. Government regulations recommend Kato-Katz's quantitative method forthe diagnosis of STHs infection. Kato-Katz's domestic product is not availableyet. This research aims to create a laboratory-scale modified Kato-Katz kit for early detection of STHsinfection by fecal egg counts (FECs) in estimating the number of eggs per gram of stool (EPG).An experimental study was conducted to create a resin template, spatulas, and 3M-adhesive tape as a modified Kato-Katz Kit. 120 Kato-Katz thick smear preparations were done from 30 samples for modified and standard Kato-Katz with duplication. The amount of stool for each was weighed. All Kato-Katz thick smear microscopic examination was done at least 60 minutes by identifying the STHs eggs and counting them.The result for both methods showed no differences in EPG for Trichuris trichiura eggs p-value=0.012 (p<0.05), meanwhile the differences for Ascaris lumbricoides eggs p-value=0.156, and Hookworm eggs p-value=0.102 (p>0.05). Under microscopic examination, some A.lumbricoides eggs morphology was not visible because the adhesive in modified Kato-Katz blurry it, and the Hookworm fragile eggshells made it disappear within less than 60 minutes.The study concluded no difference in EPG for T.trichiura eggs by FECs betweenmodified Kato-Katzas this study product with the standard. It is recommended to improve modified Kato-Katz in further study, especially the adhesive tape, before using it as a quantitative toolforearly detection of STHs infection.

Pemanfaatan Pool Serum sebagai Bahan Kontrol Ketelitian Pemeriksaan Glukosa Darah muhammad muslim; yayuk kustiningsih; endah yanuarti
Medical Laboratory Technology Journal Vol. 1 No. 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Efforts to efficiency in the implementation of the medical laboratory's internal quality control is done with the use of control serum pool. Pool control serum is a material made from leftover serum of patients who generally discarded because it is not used anymore .. The purpose of the study to determine the accuracy of the quality of the pool serum as compared with the control materials for the plant control serum glucose in improving internal quality control. Type analytic survey research with comparative approach. Analysis of data using the value of CV (coefficient of variation) and the Wilcoxon test. The results showed a decrease in serum pool of blood glucose levels after 30 days of storage by 4.7%. Based on a statistical test serum taken with the pool serum obtained significance value of 0.000 where this value is less than the value of α = 0.05, which means that there is a difference between serum taken with the pool serum. In the control serum before and after storage decreased blood glucose levels of 4.5% and is based on statistical test obtained significance value of 0.073 where the value is more than the value of α = 0.05, so there is no difference between the control serum before and after storage. Based on the calculation, the value of CV (coefficient of variation) of 5.4% serum pool and a CV (coefficient of variation) control serum by 11.6%. Accuracy pool serum and serum control exceeds the limits CV (coefficient of variation) a maximum of 5%. It is suggested further research on how long endurance pool serum as a control.

Potention of Active Charcoal from Musa Paradisiaca and Manihot Utilissima Shell in Degrading River Contamination Nurlailah Nurlailah; Anny Thuraidah; Yayuk Kustiningsih
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

River water is one of type water surface which is a lot of finding in South Kalimantan. Generally, this water used by the citizen for cooking and bathing. But along with era growth, this river becomes as disposal of various industrial waste. The contamination of heavy metal like Fe, Mn, Pb, Cd, and turbidity enhance progressively and degrade the water quality. Some natural substance which can be used to improve river water quality is active charcoal from Musa paradisiaca and Manihot utilissima shell which is easy to get. Target research is determined to find optimum dose of active charcoal from Musa paradisiaca and Manihot utilissima shell that capable to degrade heavy metal contamination and turbidity in water river. It used experimentally with pretest and post-test with control group design. A result of research showed the optimum dose of active charcoal Musa paradisiaca shell was 15 gram , it could absorb Pb 28,8% and Mn 24,6%, but 10 gram can degrade turbidity until 83,8 %, while the optimum dose of Manihot utilissima shell to 25,4% Pb and degraded 77,5% of turbidity was 15 gram. Statistical test result with Kruskal Wallis got the p-value less than 0,05 it meant there was a difference between the treatment of active charcoal from Musa paradisiaca and Manihot utilissima shell in degrading the contamination. A conclusion is those active charcoal having a potential to enhance the water river quality. Suggested to use another activator to the potential like stirring, time of contact and the different mass.

Hubungan Kadar Magnesium dengan Tekanan Darah pada Lansia di Posyandu Lansia Muna Aufa; Yayuk Kustiningsih; Nurlailah Nurlailah
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.22

Penelitian ini bertujuan untuk mengetahui adanya hubungan kadar magnesium dengan tekanan darah pada lansia di posyandu lansia Puskesmas Sungai Ulin. Penelitian ini bersifat survei analitik dengan rancangan cross-sectional. Pemeriksaan kadar magnesium menggunakan metode colorimetric test. Hasil rata-rata pemeriksaan kadar magnesium adalah 1,59 mg/dL dengan nilai normal kadar magnesium adalah 1,6-3,0 mg/dL, sedangkan hasil pengukuran rata-rata tekanan darah sistolik adalah 142 mmHg dengan nilai normal 90-119 mmHg, dan rata-rata tekanan darah diastolik adalah 90 mmHg dengan nilai normal 60-79 mmHg. Hasil uji korelasi Spearman diketahui nilai signifikansi antara kadar magnesium dengan tekanan darah sistolik dan diastolik adalah (p)=0,000 dan 0,005 dengan kekuatan hubungan (r)= -0,644 dan -0,455. Kesimpulan penelitian ini adalah ada hubungan signifikan antara kadar magnesium dengan tekanan darah sistolik (p=0,000) dan diastolik (p=0,005) pada lansia di posyandu lansia Puskesmas Sungai Ulin. Peningkatan kadar magnesium diikuti dengan penurunan tekanan darah, dan sebaliknya penurunan kadar magnesium diikuti dengan peningkan tekanan darah.

PERBANDINGAN KADAR ALANINE AMINOTRANSFERASE BERDASARKAN FREKUENSI TRANSFUSI DARAH PASIEN TALASEMIA β MAYOR DI RSD BANJARBARU Rahmawati; Neni Oktiyani; Yayuk Kustiningsih; Jujuk Anton Cahyono
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.95

Talasemia merupakan kelainan darah bawaan yang diakibatkan adanya gangguan sintesis hemoglobin di dalam sel darah merah yang mengharuskan mereka untuk mendapatkan transfusi secara rutin. Transfusi yang berulang-ulang dapat menyebabkan penumpukan zat besi dalam tubuh terutama organ hati, sehingga menyebabkan kadar Alanine Aminotransferase meningkat. Penelitian bertujuan mengetahui perbandingan kadar ALT berdasarkan frekuensi transfusi darah pada penderita talasemia β mayor di RSD Banjarbaru. Penelitian ini menggunakan jenis penelitian survei analitik. Teknik pengambilan menggunakan teknik accidental sampling. Hasil penelitian sebanyak 9 orang (30%) subjek penelitian memiliki kadar ALT diatas normal. Hasil pemeriksaan kadar ALT transfusi darah 2 minggu sekali kisaran 15-70 U/L, 3 minggu sekali kisaran 30-51 U/L, dan 4 minggu sekali kisaran 12-41 U/L. berdasarkan Uji One Way Anova didapatkan signifikansi (p = 0,044) yang berarti terdapat perbedaan bermakna paling tidak antar 2 kelompok. Kesimpulan ada perbedaan yang bermakna antara kadar ALT dengan frekuensi transfusi darah.

CEGAH PENULARAN PENYAKIT HEPATITIS B PADA IBU HAMIL MELALUI PENINGKATAN PENGETAHUAN, DETEKSI DINI DAN PHBS DI WILAYAH PUSKESMAS SUNGAI TABUK I KABUPATEN BANJAR Norsiah, Wahdah; Tini Elyn Herlina; Yayuk Kustiningsih
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 4 No 1 (2025): Jurnal Rakat Sehat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar

Hepatitis B infection is a clinical or pathological symptom characterized by various inflammations of the liver caused by Hepatitis B virus (HBV) infection (Siswanto, 2020) which can be transmitted through horizontal and vertical transmission. Horizontal transmission occurs from one person to another which can be transmitted through bodily fluids, sexual intercourse, and use of shared items. While vertical transmission occurs from pregnant women who are positive for the Hepatitis B virus to their fetuses, which can be transmitted through the placenta, during childbirth, and breast milk (Jalaluddin, 2020). This community service applies the research results of Safitri and Nurulhuda (2023) who obtained the results of HBsAg and Anti HBS examinations in pregnant women in Lok Buntar Village and Pematang Panjang Village in the Sungai Tabuk 1 Health Center area, Banjar Regency, 3 respondents were HBsAg reactive and 5 people were positive for Anti HBs. The problems experienced by partners include low levels of knowledge and understanding of clean and healthy living awareness to prevent transmission of hepatitis B, suboptimal empowerment of productive-age women and pregnant women, reactive hepatitis B test results are still found, and the unavailability of anti-HBS tests to determine the presence of hepatitis antibodies in pregnant women. The purpose of this community service activity is to educate and empower productive-age women and pregnant women who are active in the Bumil Sehati class activities. Cadres are equipped with knowledge about hepatitis B, how to prevent and treat hepatitis B infections and how to detect early occurrences of hepatitis B infections through physical examinations. This activity is carried out for 5 months from June to October 2024 with stages in the form of planning, implementation, evaluation and monitoring Evaluation to see an increase in knowledge about preventing hepatitis B in pregnant women, to see changes in healthy and clean living behavior in pregnant women. Availability of technical brochures for implementing healthy and clean living behavior to prevent transmission of Hepatitis B in pregnant women. From the results of the examination, 1 pregnant woman was found to be reactive to HBsAg and the results of the Anti-HBS examination were 3 people reactive. Handling and treatment of pregnant women who are positive for hepatitis are handed over to the midwives of the Community Health Center. The types of outputs produced in this PkM activity are 1) the formation of Bumil Sehati Cadres, 2) increased knowledge, 3) improved health of partners 4) Documentation in the form of videos and 5) Publication of the print media Radar Banjar. While additional outputs are in the form of cooperation between Poltekkes Kemenkes Banjarmasin and Bumil Sehati partners. Keyword: hepatitis B, anti HBs, pregnant woman

Perbedaan Hasil Tes Darah Rutin Pasien Suspek Covid-19 Terkonfirmasi Positif Dan Negatif Di RSUD Sultan Suriansyah Banjarmasin Yayuk Kustiningsih; Ida Fitriyani; Erfan Roebiakto; Anny Thuraidah; Nurlailah
Jurnal Praba : Jurnal Rumpun Kesehatan Umum Vol. 3 No. 3 (2025): September : Jurnal Praba : Jurnal Rumpun Kesehatan Umum
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/praba.v3i3.576

The purpose of this study was to determine the differences in routine blood in COVID-19 suspected patients who were confirmed positive and negative who were treated at the Emergency Room of Sultan Suriansyah Hospital Banjarmasin in the period January-June 2022. This study used naso-oropharyngeal swab samples for RT-PCR examination of COVID-19 and hematology analyzer for routine blood tests. This type of research is an analytic survey research with a comparative design. The population of this study were suspected patients admitted to the emergency room with a total sample size of 196 patients who performed RT-PCR tests and performed routine blood tests at the beginning of the treatment period using purposive sampling method. Routine blood results in positive patients mostly obtained leukocytes at normal limits 64.1%, neutrophilia 59.0%, lymphopenia 64.1% increased NLR 66.7% and platelets at normal limits 56.4%. While in negative patients, leukocytes were obtained at normal limits of 46.6%, neutrophilia 61.0%, lymphopenia 61.0% increased NLR 73.7% and platelets at normal limits 64.4.4%. Based on the results of the unpaired T-test statistical test, it is known that there are significant differences between positive and negative patients, namely in the parameters of leukocytes sig. 0.028, neutrophils sig. 0.043 and platelets sig. 0,005. From the results of the unpaired t-test statistical test on routine blood between positive and negative COVID-19 patients, it was found that there were significant differences in the parameters of leukocytes, neutrophils and platelets, while those that were not different were the parameters of lymphocytes and NLR.

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