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All Journal Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Riau Biologia Journal of Educational Sciences
Dita Deanesia
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Religion Agriculture, Biological Sciences & Forestry Humanities Education Library & Information Science Mathematics Other

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Analisis Sekuen Ekson 6-8 dari Gen Ferritin pada Tiga Varietas Padi (Oryza sativa l.) Asal Kecamatan Bantan, Bengkalis - Riau ROSLIM, DEWI INDRIYANI; DEANESIA, DITA; , HERMAN
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

One of the obstacles encountered in Bengkalis agricultural is the high content of Fe in the soil. Fe ionscan cause toxicity in plants if it is accumulated in an amount exceeding the tolerable limit of the plant.This study was aimed to compare the nucleotide sequences of the ferritin2 gene in some rice genotypesfrom Riau commonly growing in tidal land in Bengkalis with Fe-overload-tolerant rice variety (Mahsuri)and national rice variety which was Fe- overload -sensitive (IR64). The research was conducted at theGenetic Laboratory, Faculty of Mathematical and Natural Sciences, Riau University from January to May2014. The methods included DNA isolation, PCR, electrophoresis, and sequencing. DNA sequence dataanalysis was performed by using the BLAST and MEGA programs. This study got ± 750 bp DNAfragment of the ferritin2 gene which covering exons 6-8. Total number of SNPs obtained are as many as53 pieces and only found in introns and exons six. The farthest genetic distance was between Mahsuri andAmat Candu (0.171), followed by Sadani and Opium Amat (0.164). The shortest genetic distance wasbetween Korea and IR64 and also Sadani. Korea had the shortest distance to Nipponbare, otherwiseAmat Candu was the farthest distance from Nipponbare. Based on exons 6 to 8 sequence, Amat Canduwas classified as Fe- overload -sensitive rice variety, whereas Korea was a Fe- overload-tolerant ricevariety. Sadani was Fe-overload-moderate rice variety.Keywords : Riau, PCR, ferritin gene, Oryza sativa¸ tidal land
ISOLASI DNA TANAMAN PADI (Oryza sativa L.) ASAL KECAMATAN BANTAN, BENGKALIS – RIAU. Dita Deanesia; Dewi Indriyani Roslim; Herman '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Excess  of  Fe  in  costal  land  in  Riau Province  is potential to  poison the  roots  of  plants. However,the plants have their  own  mechanism  to overcome  the Fe overload.  This  study was aimed  to  isolate  the total   DNAof rice plants among  three  local  rice genotypes  from Riau  which are  commonly  planted in costal  land  in  Bengkalis,  Riau, with  Fe overload resistant  swamp  rice variety  (Mahsuri)  and Fe overload sensitive rice variety  (IR64). The experiment was conducted  in the Laboratory of Genetics, FMIPA UR,from January to May   2014. Research methods  consisted of  isolation  of DNA  and  electrophoresis.  In this study  isolation of DNA  from five rice  varieties  was succesfully done. Among those DNA, concentration  of  total DNA  which were  obtained from IR64 and Amat Candu were very high.
Analysis of the Misconceptions of Class XII High School Students in Pekanbaru by Using the Certainty of Response Index (CRI) Deanesia, Dita; Wulandari, Sri; Zulfarina, Zulfarina
Journal of Educational Sciences Vol 5. No. 3. July 2021
Publisher : FKIP-Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jes.5.3.p.439-447

Abstract

One of the misconceptions can be identified by using CRI (Certainty of Response Index). The purpose of this study was to identify the misconceptions of class XII high school students in the city of Pekanbaru on the material of Photosynthesis and Plant Respiration using the Certainty of Response Index (CRI) method. This research is a survey research. The location of this research was carried out at two high schools in Pekanbaru, namely SMA N 15 Pekanbaru and SMA IT Az-Zuhra Pekanbaru. The number of samples in this study is the total population of the two SMAs. The data collection technique was carried out using a written test instrument in the form of a multiple choice test accompanied by CRI. The average percentage of students' misconceptions of SMA IT AZ-ZUHRA and SMAN 15 Pekanbaru on the concept of photosynthesis, the highest percentage is in the sub-concept of the benefits of photosynthesis for plants by 96.15% and the lowest percentage is in the sub-concept of photosynthetic pigments at 53.19%. The average percentage of students' misconceptions at SMA IT AZ-ZUHRA and SMAN 15 Pekanbaru on the concept of plant respiration, the highest percentage is found in the sub-concept of understanding respiration in plants of 84.62%. The lowest percentage is found in the sub-concept of understanding breathing and the sub-concept of when plants breathe, each of which is 67.58%.
Co-Authors , HERMAN Dewi Indriyani Roslim Dewi Indriyani Roslim Herman ' Sri Wulandari Zulfarina, Zulfarina