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Steffi Ong
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Biochemistry, Genetics & Molecular Biology Veterinary

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MP-15 Characterization of Infectious Bursal Disease Isolate with Propagation in Chicken Embryonated Eggs and Molecular Biology . Syamsidar; Arini Nurhandayani; Steffi Ong; Aprilia Kusumastuti; Ardi Budi Prakoso; Gusti Ayu Yunanti Kencana
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Infectious Bursal Disease (IBD), also known as Gumboro disease is one that has an adverse economic effect. This disease is a threat to the poultry industry (Mueller, 2003). Deaths in chickens caused by IBD can even reach 100%. Outbreaks remain widespread despite vaccination programmes (Soedoedono, 2001). In Indonesia, the IBD disease is the cause of high death rates in poultry from the very virulent IBD (vvIBD) throughout the year 2014. Prevention of the disease can be done through vaccination. However, a tight vaccination programme so not ensure the safety of poultry from IBD. Therefore, there is a need for a vaccine strain to be suitable with those in the field. This can be accomplished with collecting virus isolates from the field and identifying the suitable vaccine strain. The purpose of this research is to characterize virus IBD isolates through propagation in embryonated chicken eggs. In addition, molecular methods will be used from PCR to identification with sequencing.
Respons Antibodi terhadap Penyakit Tetelo pada Ayam yang Divaksin Tetelo dan Tetelo-Flu Burung (NEWCASTLE DISEASE/ND ANTIBODY RESPONSE OF CHICKENS VACCINATED WITH ND SINGLE AND COMBINED ND AND AVIAN INFLUENZA VACCINES) Gusti Ayu Yuniati Kencana; Nyoman Suartha; Mesakh Parlindungan Simbolon; Arini Nur Handayani; Steffi Ong; Syamsidar .; Aprillia Kusumastuti
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to investigate antibody response of specific pathogen-free (SPF) chickens vaccinatedwith single inactivated Newcastle disease (ND) vaccine and combined inactive ND and avian influenza(AI) vaccines and to known the efficacy of both vaccines. The vaccines used were killed ND vaccine andkilled ND-AI vaccine produced by PT. Sanbio Laboratories Bogor, West Java. SPF chickens were vaccinatedwith 3 different doses. Antibody titer of SPF chickens against ND virus were determined byhaemagglutination inhibition (HI) test. As many as 130 two week old SPF chickens were used and theywere divided into 2 groups (A and B) consisting of 60 chickens and 10 chickens were used as control withoutvaccine. Group A chickens were vaccinated with ND-K vaccine and group B were vaccinated with combinedkilled ND-AI vaccines. Each group was further divided into 3 subsgroups (1, 2 and 3) consisting 20 chickens.Subgroups 1, 2 and 3 were vaccinated intramuscularly respectively with intramuskular 1, 1/10 and 1/100doses of each vaccines. Antibody response of chickens against ND virus was examined before vaccinationand every three week after vaccination and was expresses as geometric mean titre (GMT) HI units. Theresult showed that the titre antibody against ND increased at the second week following the vaccination.The antibody titer against ND virus of chickens vaccinated single killed ND at the second week in eachdose were 6.05 GMT HI unit, 4.05 GMT HI unit, and 0.9 GMT HI unit. The antibody titre at the third week were 7.90 GMT HI unit ,5.40 GMT HI unit and 2.20 GMT HI unit. The antibody titre against ND virus ofchickens vaccinated with combined ND-AI vaccine at the second week were 6.30 GMT HI unit , 4.15 GMTHI unit , and 2.05 GMT HI unit. At the third week, the antibody titre against ND virus of chickensvaccinated with combined ND-AI vaccine in each subgroup were 7.45 GMT HI unit, 5.60 GMT HI unit , and2.40 GMT HI unit . It showed that the antibody titers at single doses of killed ND vaccine (7.90 GMT HIunit) and combined ND-AI vaccine (7.45 GMT HI unit) at the third week after vaccination were both stilleffective as both titres were above standard protective titre.
Co-Authors . Syamsidar Aprilia Kusumastuti Aprillia Kusumastuti Ardi Budi Prakoso Arini Nur Handayani Arini Nurhandayani Gusti Ayu Yunanti Kencana Gusti Ayu Yuniati Kencana Mesakh Parlindungan Simbolon Nyoman Suartha Syamsidar .