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All Journal ODONTO Dental Journal Padjadjaran Journal of Dentistry Padjadjaran Journal of Dental Researchers and Students Odonto dental journal
Ratna Indriyanti
Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Daya antibakteri asam palmitat bawang putih (Allium sativum) terhadap Streptococcus mutans ATCC 25175Antibacterial activity of garlic (Allium sativum) palmitic acid towards Streptococcus mutans ATCC 25175 Nadya Oktarina Hendy; Ratna Indriyanti; Meirina Gartika
Padjadjaran Journal of Dental Researchers and Students Vol 4, No 2 (2020): Oktober 2020
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v4i2.27595

Abstract

Pendahuluan: Bawang putih sering digunakan masyarakat untuk mengobati berbagai penyakit termasuk yang disebabkan oleh bakteri. Bawang putih (Allium sativum) mengandung asam palmitat yang diduga memiliki aktivitas antibakteri terhadap Streptococcus mutans melalui lisis membran sitoplasma bakteri dan dapat menghambat aktivitas kerja enzim bakteri. Tujuan penelitian ini untuk mengetahui daya antibakteri asam palmitat bawang putih (Allium sativum) terhadap pertumbuhan bakteri Streptococcus mutans ATCC 25175. Metode: Penelitian bersifat deskriptif. Analisis kemurnian isolat menggunakan kromatografi lapis tipis (KLT), kemudian dilakukan uji zona hambat terhadap Streptococcus mutans menggunakan metode difusi agar Kirby-Bauer pada medium agar Mueller Hinton. Kontrol penelitian adalah klorheksidin. Zona hambat yang terbentuk di sekitar paper disk diukur menggunakan jangka sorong. Hasil: Hasil KLT menunjukkan adanya senyawa asam palmitat sebagai senyawa aktif. Asam palmitat bawang putih pada konsentrasi 0,5%, 2%, 4%, 8%, dan 12% tidak memiliki zona hambat terhadap Streptococcus mutans dan memperlihatkan gumpalan putih berupa asam lemak di sekeliling paper disk. Zona hambat klorheksidin menunjukkan rata-rata 18 mm. Simpulan: Asam palmitat bawang putih (Allium sativum) tidak memiliki daya antibakteri pada konsentrasi yang 0,5%, 2%, 4%, 8%, dan 12% terhadap Streptococcus mutans ATCC 25175. Kata kunci: Zona hambat asam palmitat bawang putih, Streptococcus mutans ATCC 25175, disc diffusion. ABSTRACTIntroduction: Garlic is often used by people to treat various diseases, especially bacterial infection disease. Garlic (Allium sativum) contains palmitic acid, which is assumed to have the antibacterial activity against Streptococcus mutans through bacterial cytoplasmic membrane lysis and inhibits the activity of bacterial enzymes. The purpose of this study was to determine the antibacterial activity of garlic (Allium sativum) palmitic acid on the growth of Streptococcus mutans ATCC 25175. Methods: This research was descriptive. Purity analysis of the isolated compound of palmitic acid was performed using a thin layer chromatography (TLC). The inhibition zone test of Streptococcus mutans was carried out afterwards, using the Kirby-Bauer diffusion method on the Mueller Hinton agar medium. The research control was chlorhexidine. A calliper measured the inhibition zone formed around the paper disk. Results: The TLC results showed that the isolated active compound was palmitic acid. Garlic palmitic acid in the concentrations of 0.5%, 2%, 4%, 8%, and 12% did not have inhibitory zone towards Streptococcus mutans and showed white lumps of fatty acids around the paper disk. Chlorhexidine showed an inhibition zone with an average of 18 mm. Conclusion: Garlic (Allium sativum) palmitic acid has no antibacterial activity towards Streptococcus mutans ATCC 25175.Keywords: Inhibitory zone of garlic palmitic acid, Streptococcus mutans ATCC 25175, disc diffusion.
PERBEDAAAN PENURUNAN MASA BIOFILM Streptococcus mutans ANTARA PEMBERIAN FRAKSI N-HEKSANA DAN ETIL ASETAT EKSTRAK ETANOL BAWANG PUTIH SIUNG TUNGGGAL (Allium sativum L.) Ratna Indriyanti; Faizal Hasan; Meirina Gartika
ODONTO : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.6.0.21-28

Abstract

Background: Garlic (Allium sativum L) is one of the most important Allium species consumed worldwide and has been used for decades as a cure for various diseases. The aim of this research was to compare the efficacy of single-clove garlic’s n-hexane and ethyl acetate fractions in inhibiting the growth of Streptococcus mutans biofilmMethod: This was a true experimental research. The biofilm mass was determined by absorbance value at 590 nm wavelength with ELISA reader in a microplate using safranin. Data was analyzed using ANOVA followed by post hoc analysis with ρ value <0.05.Result: The result showed that average decrease of biofilm mass after 1-30 minutes of ethyl acetate fraction administration was 33.4 ± 8.03 mg/mL, n-hexane 23.6 ± 0.97 mg/mL and chlorhexidine 35.5 ± 0.98 mg/mL. The average decrease of biofilm mass after 30-60 minutes of ethyl acetate fraction administration was 11.94 ± 7.44 mg/mL, n-hexane 43.87 ± 41.6 mg/mL and chlorhexidine 16.35 ± 4.6 mg/ mL. Statistic analysis showed that there was a notable difference in the decrease of Streptococcus mutans biofilm mass (ρ value= 0.00). Conclusion: The conclusion of this research was that administrating either n-hexane or ethyl acetate fractions of a single-clove garlic has the ability to decreaseS. mutans biofilm mass.
An illustration of speech articulation impairment in children with cerebral palsy tested by the Goldman-Fristoe method Ade Pungky Rusmarini; Edeh Rolleta Haroen; Ratna Indriyanti
Padjadjaran Journal of Dentistry Vol 21, No 1 (2009): March 2009
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.175 KB) | DOI: 10.24198/pjd.vol21no1.14089

Abstract

Seventy percent of children with cerebral palsy were found to suffer from speech articulation impairment. The purpose of this research was to obtain an illustration of speech articulation impair­ment in children with cerebral palsy tested by the Goldman-Fristoe method at the SLB-D School for Dis­abled Children Bandung in 2007. This was a descriptive research. Sampling was carried out by purposive sampling. The speech articulation impairment test was carried out on the basis of the Goldman-Fristoe method, that is, an articulation test which places the consonant at the beginning, middle, and at the end of a word, to test speech articulation impairment in children with cerebral palsy. Research results indicated that speech articulation impairment in the bilabial consonants /p/,/b/, and /m/ is the average 85.51%. Speech articulation impairment of the labiodental consonants /f/ and /v/ is an average 89.13%. Speech articulation impairment of the alveolar or dental consonants /t/ and /d/ is an average of 80.43%. Speech articulation impairment in the palatal consonants /c/ is an average of 82.60%. Speech articulation impairment in velar consonants /k/ and glottal consonants /h/ is an average of 86.96%. Re­search results indicated that more than three-fourths of children with cerebral palsy at the SLB-D School for Disabled Children Bandung in 2007 suffered from speech articulation impairment.
PERBEDAAAN PENURUNAN MASA BIOFILM Streptococcus mutans ANTARA PEMBERIAN FRAKSI N-HEKSANA DAN ETIL ASETAT EKSTRAK ETANOL BAWANG PUTIH SIUNG TUNGGGAL (Allium sativum L.) Ratna Indriyanti; Faizal Hasan; Meirina Gartika
Odonto : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.853 KB) | DOI: 10.30659/odj.6.0.21-28

Abstract

Background: Garlic (Allium sativum L) is one of the most important Allium species consumed worldwide and has been used for decades as a cure for various diseases. The aim of this research was to compare the efficacy of single-clove garlic’s n-hexane and ethyl acetate fractions in inhibiting the growth of Streptococcus mutans biofilmMethod: This was a true experimental research. The biofilm mass was determined by absorbance value at 590 nm wavelength with ELISA reader in a microplate using safranin. Data was analyzed using ANOVA followed by post hoc analysis with ρ value <0.05.Result: The result showed that average decrease of biofilm mass after 1-30 minutes of ethyl acetate fraction administration was 33.4 ± 8.03 mg/mL, n-hexane 23.6 ± 0.97 mg/mL and chlorhexidine 35.5 ± 0.98 mg/mL. The average decrease of biofilm mass after 30-60 minutes of ethyl acetate fraction administration was 11.94 ± 7.44 mg/mL, n-hexane 43.87 ± 41.6 mg/mL and chlorhexidine 16.35 ± 4.6 mg/ mL. Statistic analysis showed that there was a notable difference in the decrease of Streptococcus mutans biofilm mass (ρ value= 0.00). Conclusion: The conclusion of this research was that administrating either n-hexane or ethyl acetate fractions of a single-clove garlic has the ability to decreaseS. mutans biofilm mass.
Co-Authors Ade Pungky Rusmarini Edeh Rolleta Haroen Faizal Hasan Meirina Gartika Nadya Oktarina Hendy