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Chemometric Analysis of Serum Magnesium Calculations Using Mg-Xylidyl Blue-I Method Based on Molar Absorptivity Ally Kafesa; Nadira Nur Hajah Lutfi; Cep Wahyu
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1876

Abstract

The concentration of magnesium is determined based on the absorbance of the Mg-Xylydil Blue-I complex solution use spectrophotometer. Based on the Lambert-Beer rule, the calculation of sample concentration is based on the formula A = Ԑ. b. C. Generally, the thickness of the cuvette (b) and the molar absorptivity (Ԑ) factor will be ignored because it is considered to have a fixed value, therefore the sample concentration is measured based on the ratio of the absorbance of the sample against the standard solution. However, the standard solution contains pure magnesium and has a different matrix than the sample matrix, so this condition can give analytical errors and lead to misinterpretation of the results. The purpose of this study was to determine the accuracy and the precision of serum magnesium calculation by the principle of the Mg- Xylydil Blue-I complex reaction based on molar absorptivity compared to the general method. This research uses comparative study design methods. The serum sample used was the patient's serum specimen who has a normal magnesium level. The results showed that the significance value of the paired t-test statistical was 0.000 (p < 0.05). The accuracy value (d%) of the calculation formula uses Ɛ is 0.00 and the precision value (CV%) is 0.53. While the accuracy value (d%) of the calculation formula without Ɛ is 0.00 and the precision value (CV%) is 0.38. Calculations based on molar absorptivity (Ɛ) can measure more significant serum magnesium than those calculated based on standard magnesium solutions.
EFEKTIVITAS INFUSA KULIT NANAS SEBAGAI BIOLARVASIDA Aedes aegypti Liah Kodariah; ally Kafesa
Jurnal Kesehatan Rajawali Vol 11 No 1 (2021): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.514 KB)

Abstract

Dengue hemorrhagic fever is a disease caused by the dengue virus which is transmitted through the bite of the Aedes aegypti mosquito. Controls are generally carried out chemically which can cause negative impacts if used continuously. Pineapple peel is a plant that can be used as a biolarvicide which contains flavonoids, saponins, tannins, and bromelain enzymes which can cause the death of Aedes aegypti larvae.This study aims to determine the concentration of pineapple peel infusion which is effective in killing Aedes aegypti larvae seen from the LC50 value.This research uses experimental methods. The sample population was larvae of Aedes aegypti. Samples were taken in the captivity of the Bandung Health Polytechnic Laboratory as many as 140 instar III Aedes aegypti larvae with purposive sampling technique. The concentration of pineapple skin infusion used was 23%, 21%, 20%, 19%, and 17%. each treatment In this study, the percentage of larvae mortality was obtained, namely at a concentration of 23% there were 65% of larvae deaths, at a concentration of 21% there were 60% deaths, at a concentration of 20% there were 50% deaths, at a concentration of 19% there were 50% deaths, and at a concentration of 20% there were 50% deaths. and at concentration of 17% contained 35% of deaths.Based on the research, it can be concluded that pineapple skin infusion can be used as an effective biolarvicide seen from the LC50 value, which is at a concentration of 19%. contained 10 test larvae with two repetitions and observed after 24 hours.
IDENTIFICATION OF ASPERGILLUS SP MUSHROOMS IN WHEAT FLOUR SOLD OPENLY IN CIROYOM MARKET AS RAW MATERIALS FOR HALAL AND MUNGLE-FREE Fitri Rahmi Fadhilah; Ally Kafesa; Sopi Napita
International Journal Mathla’ul Anwar of Halal Issues Vol. 1 No. 2 (2021)
Publisher : Universitas Mathla’ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.103 KB) | DOI: 10.30653/ijma.202112.19

Abstract

Wheat flour is used as the main ingredient of food which is often used by the wider community, one of which is as an ingredient for making various kinds of food products. Wheat flour is included in food products safe and halal for consumption. The wheat flour which is sold openly is packaged in transparent plastic. With the easy-to-open packaging, it is easy for microorganisms to enter the flour, one of them is fungus. This study was aim to determine the types of Aspergillus sp in wheat flour which is sold openly in the Ciroyom Market. Method The research used is descriptive by using 30 samples of wheat flour which are sold openly in the Ciroyom Market. This research was conducted in the laboratory of the Rajawali Health Institute, Bandung. Result and Discussion: The results of this study showed the presence of Aspergillus flavus (50%), Aspergillus fumigatus (50%), Aspergillus niger (16.7%), Aspergillus terreus (3.3%) and negative (26.7%) Aspergillus sp. Conclusion: From this research, it can be concluded that the species of Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger and Aspergillus terreus were found so we can conclude that the wheat flour that is sold openly is still a halal product but it is not mushroom-free products.