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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Biomedika Jurnal Bidan Cerdas Jurnal Pengabdian Bidan Nasuha
Minarti Minarti
D-III Kebidanan, Politeknik Baubau
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Metagenomic Diversity of Gut Microbiota of Gestational Diabetes Mellitus of Pregnant Women Minarti Minarti; Nurhidayat Triananinsi; Nurqalbi Nurqalbi; Sumarni Sumarni; Mudyawati Kamaruddin
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.747

Abstract

Gestational Diabetes Mellitus (GDM) is defined as a condition in which a woman without diabetes develops abnormal glucose tolerance that is first recognized during pregnancy. GDM is a significant public health problem with an incidence of 1.9 – 3.6% of all pregnancies in Indonesia. Additionally, women with GDM during pregnancy have a high risk of developing diabetes when they are not pregnant, such as type 2 diabetes (T2D). One alternative variable in the management of T2D globally is gut microbiota. Here, to find out the role of gut microbiota in pregnancy, we characterized the stools of 30 pregnant women, each consisting of fifteen GDM-detected pregnant women, and healthy pregnant women using metagenomic approach with genome analysis by directly isolating genomic DNA from the microbiota ecosystem that occupies the digestive tract. DNA sequencing results were analyzed by MEGA 6 software with the BLASTn algorithm in NCBI. Thus fifteen GDM-detected showed high nucleotide sequence homology with the Proteobacteria at phylum level, and Escherichia, Orchobacterium, Cronobacter, Shigella, Salmonella, Enterobacter, Klebsiella, Kosakonia, Vibrio dan Gamma-Proteobacterium at genus level compared to the healthy pregnant women which found by Firmicutes at phylum level and Ruminococcus, Clostridium, Clostridiales, Lachnospiraceae, Roseburia, Weisella, Eubacterium at genus level had a higher abundance in healthy pregnant women. In this result, we found also one of the fifteen healthy pregnant women showed differential abundance with enrichment of Prevotella species. Gut microbiota of GDM-diagnosed pregnant women has more varied composition, and dominated by the phylum Proteobacteria than in normal pregnant women.
Posisi Ibu Hamil Memengaruhi Akurasi Pengukuran Kesejahteraan Janin: Position of Pregnant Women Effects on Accuracy of Fetal Heart Rate Measurement Minarti Minarti; Risnawati Risnawati
Jurnal Bidan Cerdas Vol. 2 No. 3 (2020)
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jbc.v2i3.93

Abstract

During pregnancy, the fetal heart rate is a picture of the well-being of the fetus in the womb. The mother's position affects the results of the assessment when taking measurements of the fetal heart rate. The purpose of this study is to determine the accuracy of the measurement of fetal well-being based on the position of pregnant women. This type of research is analytic observational in Wajo Health Center, Katobengke Health Center, and Sulaa Health Center, from April - August 2019. The population in this study were all pregnant women registered in the KIA book. The research sample consisted of 60 respondents using inclusion criteria. The data analysis uses univariate analysis. The results showed there were differences in the value of the fetal heart rate measurements for each position. The average value (min-max) of DJJ measurement with the lying position (supine) 128-158 times per-minute, mean value 138.81 times per-minute; sitting position 124-158 times per-minute, mean value 143.41 times per-minute; standing position 126-159 times per-minute, mean value 145.58 times per minute. In a sitting and lying position, the resulting heart rate is in the normal range of 120-140 times per-minute; in a standing position, the heart rate obtained 150-160 times per-minute has the potential for tachycardia. The conclusion of this study is that the position of pregnant women affects the results of fetal heart rate measurement and the good position of the FHR measurement when lying on her back
Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula Mudyawati Kamaruddin; Nurhidayat Triananinsi; Nurqalbi Sampara; Sumarni Sumarni; Minarti Minarti; A. Maya RA
Media Kesehatan Masyarakat Indonesia Vol. 16 No. 1: MARET 2020
Publisher : Faculty of Public Health, Hasanuddin University, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.469 KB) | DOI: 10.30597/mkmi.v16i1.9050

Abstract

Breast milk is the best source of nutrition because it contains a variety of healthy compounds and immunity. Several studies of gut microbiota has proven its important role in the development of the immune system. This study aims to characterize the gut microbiota of babies born normally with breastfeeding and formula milk. This research using cross sectional study design with purposive sampling as sampling technique. Samples used were babies born normally as many as 30 infants that divided into two groups namely infants who given breast milk and combinated breastfeeding and formulamilk of 15 infants each. Stool samples were taken by swab technique, the DNA was extracted using a DNA kit continued DNA amplification by PCR with 16SrRNA universal primers then DNA sequencing. Data from DNA sequencer analized using a software of MEGA5 and BLAST attained on the NCBI link. The result showed that the group of infants who were breastfed plus formula milk had more varied intestinal microbiota than the group of infants who were only given breast milk. It can be concluded that intestinal microbiota that dominates the intestines of infants who are given breast milk can be a probiotic in the growth of the baby and the development of the immune system.
Strengthening the Role of Women in Preventive against Covid-19 Efforts in Kampung Tenun, Topa Village, Baubau: Penguatan Peran Perempuan dalam Upaya Preventif Covid-19 di Kampung Tenun Desa Topa, Baubau Wa Ode Nesya Jeni Samrida; Minarti Minarti; Syastriani Isna Putri Syarif
Jurnal Pengabdian Bidan Nasuha Vol. 2 No. 2 (2022)
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jpbn.v2i2.1113

Abstract

Introduction: The spread of the COVID-19 virus is increasing, especially in Kampung Tenun, Topa Village, Baubau. Women take a big role in ensuring the health of the family. All information that is a source of improving family health becomes part of the role of women as wives, mothers and children. The purpose of this activity is to ensure that women have the correct information about COVID-19 so that their families are well educated and healthy during the pandemic. Methods: The implementation of the activity uses the community participation method with indicators of community participation in activities, namely first through counseling about covid-19 and how to prevent it, a dissertation on pre-posttest evaluation, hand washing practices and fitness exercise activities for 60 minutes with an instructor. participants totaled 30 people. The results of the counseling activity showed that after receiving counseling related to Covid-19 prevention, there was an increase in the knowledge of the women of the Topa Village Weaving Village, being able to practice good and correct hand washing and enthusiastically participating in counseling activities. fitness training hopes to become a resident's routine. We hope that activities involving women must be sustainable because women's role is very large for health starting at home (family).
Co-Authors A Maya Rupa Anjeli Dewi Andariya Ningsih Dina Raidanti Leila Nisya Ayuanda Mudyawati Kamaruddin Mudyawati Kamaruddin Nurhidayat Triananinsi Nurhidayat Triananinsi Nurqalbi Nurqalbi Nurqalbi Sampara Risnawati Risnawati Samrida, Wa Ode Nesya Jeni Sumarni Sumarni Sumarni Sumarni Syastriani Isna Putri Syarif Wahidin, Wahidin