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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES The Indonesian Biomedical Journal Universa Medicina Jurnal Kedokteran dan Kesehatan MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan
Afiana Rohmani
Fakultas Kedokteran Universitas Muhammadiyah Semarang
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health

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Kersen Leaf (Muntingia Calabura L.) Extract Prevents Gastric Damage of Wistar Rats Exposed to 40% Ethanol Dyah Ayuning Permatasari; Arum Kartikadewi; Afiana Rohmani; Noor Yazid
Saintika Medika Vol. 16 No. 2 (2020): December 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.Vol16.SMUMM2.11368

Abstract

Latar Belakang: Mengurangi stres akibat oksidatif dan mengurangi risiko mukosa lambung. Daun Kersen dikenal sebagai antioksidan. Penelitian ini bertujuan untuk mempelajari efek ekstrak daun Kersen untuk mencegah kerusakan lambung pada tikus Wistar yang diinduksi alkohol.Metode : Penelitian ini adalah eksperimental laboratorium dengan menggunakan desain kelompok Post test saja. Sampel terdiri dari 24 tikus wistar jantan yang dibagi menjadi 4 kelompok, yaitu K-diberikan plasebo aquadest 1,8ml / 200g / hari, K + diberi etanol 40% dosis 1,8ml / 200g /, P1 dan P2 adalah tikus yang diinduksi dengan alkohol 40% dan diberikan dengan ekstrak daun Kersen (500 mg / kg berat badan untuk P1 dan 750 mg / kg berat badan untuk P2), 60 menit setelah pemberian alkohol. Skor Kerusakan lambung berdasarkan Skor Barthel-Manja. Perbedaan rata-rata pada tingkat kerusakan mukosa lambung antara kelompok yang dianalisis dengan One Way ANOVA Test dan Post hoc Lsd Test.Hasil : Rata-rata skor kerusakan lambung adalah; K- = 0,44, K + = 1,76, P1 = 0,76 dan P2 = 0,44 (p <0,001). Perbedaan derajat kerusakan mukosa lambung pada K +, P1, P2 dibandingkan dengan masing-masing K- (ρ: <0,001); (ρ: 0,143); (ρ: 1.000).Kesimpulan : Pemberian ekstrak daun Kersen dengan dosis 500mg / Kg dan 750mg / Kg mampu mencegah kerusakan mukosa lambung yang diinduksi oleh etanol 40%.
CAPN10 SNP-19 is Associated with Susceptibility of Type 2 Diabetes Mellitus: A Javanese Case-control Study Yanuarita Tursinawati; Rifqi Fauzan Hakim; Afiana Rohmani; Arum Kartikadewi; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.984

Abstract

BACKGROUND: The health data of Central Java, Indonesia showed that diabetes mellitus (DM) was the second most increasing non-communicable disease in the province. More than 20 genes have been reported to be associated with DM. Calpain-10 (CAPN10) polymorphism has been reported to be associated with Type 2 Diabetes Mellitus (T2DM). However, the association between CAPN10 single nucleotide polymorphism (SNP)-19 and T2DM among Javanese ethnics has never been reported. Therefore, this study was conducted to investigate the association.METHODS: After fasting for 8 hours, blood samples were drawn from veins of 107 T2DM and 107 non diabetic subjects. A half of the drawn blood was collected for identification of CAPN10 SNP-19, and another half for measuring triglycerides and fasting blood glucose (FBG). Identification of CAPN10 SNP-19 was performed with polymerase chain reaction (PCR) method, while measurement of triglycerides and FBG with colorimetric enzymatic method.RESULTS: The number of T2DM Javanese subjects with 2R/3R and 3R/3R CAPN10 SNP-19 genotypes was significantly higher than the number of T2DM Javanese subjects with 2R/2R genotype (p=0.002). When each number of 2R/3R and 3R/3R T2DM subjects was compared with the number of 2R/2R T2DM subjects, the number of 2R/3R T2DM subjects was significantly higher than the number of 2R/2R T2DM subjects (p=0.000).CONCLUSION: Javanese subjects with 2R/3R and 3R/3R CAPN10 SNP19 genotypes might have susceptibility of T2DM.KEYWORDS: Calpain-10, CAPN10, polymorphism, type 2 diabetes mellitus, triglycerides, fasting blood glucose
Mangosteen peel extract reduces formalin-induced liver cell death in rats Afiana Rohmani; Sarjadi Sarjadi; Winarto Winarto; Kisdjamiatun Kisdjamiatun
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.109-117

Abstract

BACKGROUNDFormalin is a xenobiotic that is now commonly used as a preservative in the food industry. The liver is an organ that has the highest metabolic capacity as compared to other organs. Mangosteen or Garcinia mangostana Linn (GML) peel contains xanthones, which are a source of natural antioxidants. The purpose of this study was to evaluate the effect of mangosteen peel extract on formalin- induced liver cell mortality rate and p53 protein expression in Wistar rats.METHODSEighteen rats received formalin orally for 2 weeks, and were subsequently divided into 3 groups, consisting of the formalin-control group receiving a placebo and treatment groups 1 and 2, which were treated with mangosteen peel extract at doses of 200 and 400 mg/kgBW/day, respectively. The treatment was carried out for 1 week, and finally the rats were terminated. The differences in liver cell mortality rate and p53 protein expression were analyzed.RESULTSOne-way ANOVA analysis showed significant differences in liver cell mortality rate among the three groups (p=0.004). The liver cell mortality rate in the treatment group receiving 400 mg/kgBW/day extract was lower than that in the formalin- control group. There was no p53 expression in all groups.CONCLUSIONSGarcinia mangostana Linn peel extract reduced the mortality rate of liver cells in rats receiving oral formalin. Involvement of p53 expression in liver cell mortality in rats exposed to oral formalin is presumably negligible.
Ekstrak Buah Kersen (Muntingia calabura) dalam Menurunkan Jumlah Sel Goblet pada Tikus yang Dipapar Asap Rokok Ika Dyah Kurniati; Afiana Rohmani
Jurnal Kedokteran dan Kesehatan Vol 13, No 2 (2017): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.13.2.144-152

Abstract

Asap rokok dapat meningkatkan stres oksidatif yang menyebabkan aktivasi dari Epidermal Growth Factor Receptor (EGFR) sehingga mencegah apoptosis sel bersilia serta mendiferensiasi sel bersilia menjadi sel goblet. Buah kersen (Muntingia calabura) mengandung antioksidan diantaranya flavonoid. Tujuan penelitian ini membuktikan pengaruh pemberian ekstrak buah kersen terhadap jumlah sel goblet pada trakea tikus. Penelitian ini merupakan penelitian eksperimental laboratorik dengan rancangan Post Test Only Control Group Desain. Tikus sejumlah 18 ekor dibagi 3 kelompok, kemudian di papar asap rokok 7 batang perhari dan ekstrak buah kersen (EBK) selama 4 minggu. Kelompok pertama sebagai kontrol (K) diberi plasebo, kelompok Perlakuan 1 (P1) diberi EBK 100mg/kgBB/hari dan kelompok Perlakuan 2 (P2) diberi EBK 200mg/kgBB/hari. Perbedaan jumlah sel goblet dianalisis dengan Kruskal-Wallis Test dilanjutkan Post Hoc Mann-Whitney U Test. Hasil menunjukkan bahwa rerata sel goblet pada kelompok perlakuan 1 (P1=14,44) paling sedikit dibandingkan kelompok perlakuan 2 (P2=15,32) dan kelompok kontrol (K=23,08). Ada perbedaan bermakna jumlah sel goblet antara kelompok K dengan P1(ρ<0.0001) dan kelompok P1 dengan P2 (ρ=0,002), namun tidak terdapat perbedaan yang bermakna antara kelompok K dengan P (ρ=0,126). Simpulannya ekstrak buah kersen mampu menurunkan jumlah sel goblet trakea tikus yang di papar asap rokok. Kata kunci: asap rokok, Muntingia calabura, sel goblet
Sexual Dimorphism of the first Lumbar vertebra in the Malaysian Population. Afiana Rohmani
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 12 No 1 (2022): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2022.v12.i01.p05

Abstract

Sex determination is one of the main steps in the identification of human skeletal remains. The vertebrae are weight-bearing structures in the human body that may provide variety of information from an individual. The aim of this study is to assess the sexual dimorphism of the first lumbar (L1) vertebrae using three-dimensional (3D) computed tomography (CT) imaging to develop population-specific equations for sex identification in the Malay population. Thirteen linear measurements of the first lumbar (L1) vertebrae were taken from 50 males and 50 females’ patients in the Radiology Department of Universiti Kebangsaan Malaysia Medical Centre, using images of the Computed Tomography (CT) scan. Independent T-test and discriminant function analysis (DFA) were performed for analysis. By using independent T-test analysis, there were eight measurements showed statistically significant difference between men and women (p<0.001). Using stepwise method of discriminant analysis showed three measurements predicted sex with the accuracy 93.0% : (a) lower end-plate width (EPWI), (b) lower end-plate depth (EPDI), and posterior height of the vertebral body (VBHp). This study provides discriminant equation for forensic identification of sex from the first lumbar vertebrae among Malaysia population with the accuracy 93.0%.
Efek Ekstrak Buah Kersen (Muntingia Calabura) terhadap Jumlah Sel Epitel Bersilia Bronkus pada Tikus Wistar yang Dipapar Asap Rokok. Afiana Rohmani; Ika Dyah Kurniati
MAGNA MEDIKA: Berkala Ilmiah Kedokteran dan Kesehatan Vol 2, No 4 (2018): August
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.739 KB) | DOI: 10.26714/magnamed.2.4.2018.49-56

Abstract

Effect of Kersen Fruit Extract (Muntingia Calabura) on the Number of Bronchial Ciliated Epithelial Cells in Wistar Rats Exposed to Cigarette SmokeLatar Belakang: Asap rokok menyebabkan stress oksidatif dan memicu  aktifitas Epidermal Growth Factor Receptor (EFGR)pada sel epitel bronkial, sehingga menyebabkan hiperplasia dan peningkatan jumlah sel epitel tersebut.  Buah kersen (Muntingia Calabura) mempunyai aktifitas antioksidan yang kuat dan diharapkan dapat menurunkan jumlah oksidan yang ditimbulkan oleh paparan asap rokok.Tujuan penelitian:  mengetahui efek ekstrak buah kersen terhadap jumlah sel epitel bersilia pada saluran nafas pada tikus wistar yang dipapar asap rokok.Metode: Tikus wistar berjumlah 24 ekor dibagi menjadi 4 kelompok : kelompok kontrol negatif (K-)diberikan  plasebo saja, kelompok kontrol positif (K+) diberikan plasebo dan dipaparkan asap rokok, kelompok perlakuan 1 (P1) dan kelompok perlakuan 2 (P2) yang dipapar asap rokok dan diberi ekstrak buah kersen per oral  dengan dosis 100 mg/kg BB /hari dan 200 mg/kgBB/hari. Pemaparan asap rokok dilakukan selama 30 menit setiap hari. Perlakuan ini dilakukan selama 20 hari  , kemudian semua binatang coba diterminasi. Kemudian dilakukan analilsis sel epitel bronkial.Hasil: Melalui analisis Kruskal Wallis menunjukkan perbedaan yang signifikan (p=0,000) antara  jumlah sel sepitel bersilia pada kelompok kontrol negatif (K-)   dengan kelompok kontrol positif (K+). Terdapat perbedaan yang signifikan (p=0,001) jumlah epitel bersilia antara kelompok kontrol positif (K+) dengan kelompok perlakuan 1 (P1).Simpulan: Pemberian ekstrak buah kersen dengan dosis 100mg/kgBB/hari memberikan efek signifikan dalam hal menurunkan jumlah sel epitel bersilia bronkial pada tikus yang dipapar asap rokok. Background: Cigarette smoke causes oxidative stress and triggers the activity of Epidermal Growth Factor Receptor (EFGR) in epithellium bronchial cells that lead to hyperplasia and increasing the number of cells. Kersen fruit (Muntingia calabura Linn) has strong antioxidant activity, was expected to decrease the amount of oxidant caused by cigarette smoke exposure. The purpose of this study was to examine the effect of kersen fruit extract on the number of ciliated bronchial epithellium cells in wistar rats exposed to cigarette smoke.Methods: 24 rats were divided into 4 groups : Negative control group (C-) received a placebo, while the positive control group (C+) received a placebo and cigarrete smoke. Both treatment groups 1 (T1) and groups 2 (T2) exposed cigarrete smoke and received kersen extract by gavage at a dose of 100mg/kgBW/day and 200 mg/kgBW/day. The cigarrete smoke were exposed for 30 minutes in each day. These intervention were carried out for 20 days, and finally the animals were terminated. The differences in bronchial epithellium cells were then analyzed.Results: The Kruskal Wallis analysis showed significant differences (p = 0,000) in the ammount of ciliated epithelium cellsin negative control group (C-) compare with positive control group (C+). There were significant difference (p = 0.001) in the ammount of ciliated epithellium cells in positive control group (C+) compare with treatment group 1 (T1).Conclusion: Receiving kersen fruit extract 100mg / kgBW has significant effect on reducing the ammount of ciliated bronchial epithellium cells in rats exposed to cigarettes.
Co-Authors Arum Kartikadewi Arum Kartikadewi Dyah Ayuning Permatasari Ferry Sandra Ika Dyah Kurniati Kisdjamiatun Kisdjamiatun Noor Yazid Rifqi Fauzan Hakim Sarjadi Sarjadi Winarto Winarto Yanuarita Tursinawati