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Preliminary Study on Potential Bacterial Species Introducing Antibacterial Agent from Sponge in Unggeh Island Central Tapanuli, Indonesia Latifah Ningrum Abdillah; Dwi Suryanto; Kiki Nurtjahja; Jendri Mamangkey
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.8264

Abstract

Sponges have the potential to symbiont with bacteria that produce antibacterial compounds. This study was conducted to isolate and test the antibacterial activity of sponge symbiont bacteria. Bacterial isolates obtained were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli using the Kirby-Bauer method. The identification results of both sponges have similarities with Clathria sp. and Hyrtios sp. symbiont bacteria obtained from both sponges as many as 16 isolates. Antibacterial activity testing had a weak inhibitory effect on isolates Sp1A, Sp2C, Sp2D and Sp2F, while Sp2J showed moderate inhibition against gram-negative and positive bacteria. The antibacterial activity of the whole supernatant of symbiont bacteria obtained the diameter of the inhibition zone with weak, medium and strong categories. Sp1A isolate supernatant was obtained, potentially able to fight Escherichia coli bacteria with a strong category as a representation of gram-negative bacteria and against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) classified into the medium category. In conclusion, 16 symbiont bacterial supernatants have antibacterial activity mostly active against gram-negative bacteria Escherichia coli and gram-positive bacteria Bacillus subtilis. There are 6 isolates (Sp1A, Sp1B, Sp1E, Sp1F, Sp2D, Sp2F) active as antibacterial against Staphylococcus aureus with weak and moderate categories.
Antimicrobial Activity Test of Methanol Extract of Haliclona sp. from Sibolga Waters Ulfa Mashitah; Dwi Suryanto; Kiki Nurtjahja
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.8287

Abstract

Haliclona sp. is a genus of demosponges in the family Chalinidae. Demospongiae are a type of sponge that lives in marine environments and is known for its unique, often branching skeletal structure. The use of antibiotics has led to the selection of pathogenic microorganisms in humans that are more resistant to many types of drugs, resulting in poorer treatment outcomes in cases of infection. Bacteria can become resistant to antibiotics due to improper dosing, inaccurate diagnosis, and inappropriate use of antibiotics against the causative bacteria. This study aims to determine the antimicrobial activity of the methanol extract of Haliclona sp. as an inhibitor of pathogenic microbes. This research employs a descriptive method with antagonistic testing. The results of the methanol extraction process of the symbiotic bacteria in Haliclona sp. produced a thick, dark brown extract. The results of the antagonistic test showed that the clear zones formed on the three test microbes were categorized as strong.
Phytochemical Screening, Activity Antioxidant and Activity Test of Antimicrobials Ethanol Extract of Gagatan Harimau Leaves (Paraboea leuserensis B.L Burtt) Ade Putrianti Marbun; Dwi Suryanto; Elimasni
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.8428

Abstract

Paraboea leuserensis BL Burtt, a plant endemic to the provinces of Aceh and North Sumatra, is traditionally used for medicinal purposes by chewing or boiling, treating conditions such as stomach aches and increasing stamina. Research This aiming to know difference activity antioxidant and antimicrobial from extract ethanol gagatan harimau leaves. Extraction was carried out using the maceration method using ethanol solvent. Phytochemical testing includes tests alkanoids, flavonoids, glycosides, saponins, tannins, steroids and triterpenoids. Antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picryl hydrazyl) method which was measured at a wavelength of 515 nm and antibacterial activity test by disc diffusion method (Kirby-Bauer Test). Test results phytochemicals show metabolit secondary contained​ in extract ethanol leaf idea tiger among them alkaloids, flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids. Extract ethanol gagatan harimau leaf own activity antioxidants that are classified as strong based on IC50 value obtained​ as big as 45.95 ppm Activity test antimicrobial In E. coli inhibition zone value highest at concentrations of 75%, and 50%., S. aureus mark the highest inhibition zone at concentrations of 75% and 50%, then in C. albicans with inhibition zone values at concentrations of 75% and 50%.