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All Journal HAYATI Journal of Biosciences Pro-Life Jurnal Penelitian Pendidikan IPA (JPPIPA) Bio-Lectura Reswara: Jurnal Pengabdian Kepada Masyarakat Spizaetus: Jurnal Biologi dan Pendidikan Biologi Indigenous Biologi International Journal Of Science, Technology & Management (IJSTM) Bioeduca : Journal of Biology Education Inteligensi : Jurnal Ilmu Pendidikan Jurnal ComunitA Servizio Bioscientist : Jurnal Ilmiah Biologi Oryza : Jurnal Pendidikan Biologi Journal of Natural Sciences International Journal of Health and Pharmaceutical (IJHP) Cocos Bio Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI
Jendri Mamangkey
Universitas Kristen Indonesia
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Nursing Physics Public Health Social Sciences Transportation Other

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Characteristics of Curds With Milk Clotting Enzyme from Indonesian Local Isolate of Lactic Acid Bacteria Putranto, Wendry; Mustopa, Apon; Mamangkey, Jendri; Aritonang, Netty
International Journal of Science, Technology & Management Vol. 1 No. 2 (2020): Juli 2020
Publisher : International Journal of Science, Technology & Management

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v1i2.18

Abstract

To get the potential of lalcat acid bacteria isolate to produce Milk Clotting Enzyme (MCE), it is necessary to screen milk clotting activity both quantitatively and qualitatively. Through qualitative observation, the characteristics of the curd resulting from enzyme activity can be obtained. MCE is a protease that has the characteristics of milking. Based on the results of this observational research, the curd characteristic produced can be used as a benchmark to determine the length of time of fermentation and optimization of the determination of ammonium sulfate precipitation concentration. Isolate BAL shows the results of a compact curd at a fermentation time of 25 hours at 37 ℃ and the optimization results of the deposition of ammonium sulfate which shows the characteristics of a compact curd by 45% ammonium sulfate.
Bioprospecting For Bacterial Endophytes Associated With Zingiberaceae Family Rhizomes In Sibolangit Forest, North Sumatera mamangkey, Jendri; Lucas William Mendes; Mada Triandala Sibero; Pilidai Endorina Nadapdap
International Journal of Science, Technology & Management Vol. 1 No. 1 (2020): May 2020
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v1i1.7

Abstract

The present study was conducted aiming to isolate and characterize endophytic bacterial isolates with antibacterial ability, phosphate solubilization, and proteolytic activity from rhizomes of the Zingiberaceae family (Etlingera sp., Globba patens, Globba pendula, and Zingiber multibracteata). Nineteen bacterial isolates were obtained from Zingiberaceae rhizomes with isolate codes of EZS27, EZS18, EZS19, EZS25, EZS16, EZS08, EZS09, EZS13, EZS20, EZS14, EZS10, EZS11, EZS03, EZS05, EZS06, EZS43, EZS45, EZS47, and EZS28. The screening of the endophytes for antibacterial activity was done through the paper disc method. Four bacterial isolates presented antibacterial activities. EZS06 isolate inhibited the growth of EPEC (11 mm), P. vulgaris ATCC 13315 (10 mm), and L. monocytogenes BTCC B693 (9 mm). Also, EZS20 isolate inhibited the growth of S. aureus ATCC 29213 (17 mm), EZS28 isolate inhibited MRSA ATCC 43300 (8.6 mm), and EZS45 isolate inhibited S. Epidermidis ATCC 12228 (9 mm). The EZS19, EZS03, and EZS16 isolates dissolved the phosphate most effectively. Eight isolates (EZS19, EZS47, EZS27, EZS25, EZS09, EZS20, EZS45, and EZS06) showed the best protease activity. In general, our results showed that the endophytic bacterial strains can be used as a new and useful antibacterial agent since it showed antibacterial activity and chemical diversity. Furthermore, it also has the potential for exploitation in a wide variety of medical, agricultural, and industrial areas.
Endophytic Bacteria and Fungi from Indonesian Medicinal Plants with Antibacterial, Pathogenic Antifungal and Extracellular Enzymes Activities: A Review Jendri Mamangkey; Lucas William Mendes; Arman Harahap; Dana Briggs; Cengiz Kayacilar
International Journal of Science, Technology & Management Vol. 3 No. 1 (2022): January 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i1.428

Abstract

The habitat of endophytic bacteria in plant tissues making it automatically used by plants to protect themselves against pathogenic bacteria. Endophytic bacteria have been applied in various fields, including agriculture, pharmacy, medicine, and biotechnology. This review was carried out to summarize recent studies focusing on the diversity of endophytic bacteria derived from traditional medicinal plants which have antibacterial activity and extracellular enzymes. This article was prepared and written by referring to literature studies collected from books and online journal publication. The literature review of endophytic bacteria focused on Vernonia anthelmintic, Saurauia scaberrinae, and Ki rinyuh (Chromolaena odorata) plants. The results revealed that endophytic bacteria obtained from Vernonia anthelmintic plant is from Micrococcus, Bacillus, Pseudomonas, Stenotrophomonas, and Pantoea genus which possess the same biological properties as the host plant, producing antibacterial activity. Saurauia scaberrinae plant successfully isolated endophyte fungus Phoma sp. which produces phomodione (4) and cercosporamide. Phomodione (4) exhibit inhibitory activity against S. aureus, P. ultimum, S. sclerotiorum and R. solani. Cercosporamide exhibits inhibitory activity against S. aureus. Endophytic bacteria were also isolated from Ki rinyuh (Chromolaena odorata), including BECB3, BECB 4, BECA 8, BECA 5, BECA 1, and BECA 10 isolates which have extracellular enzyme activities such as β-amylase, α-amylase, cellulase, chitinase, and protease. Understanding endophytic bacteria in medicinal plants can help researchers apply them effectively.
AKTIVITAS PROTEOLITIK BAKTERI LOKAL YANG DI ISOLASI DARI TEMPAT PENANGKARAN BUAYA Jendri Mamangkey; Dwi Suryanto
Jurnal Pro-Life Vol. 8 No. 2 (2021): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Indonesia has an ecological diversity which can be used as significantly potential habitat for local bacteria. One of such environments is crocodile breeding where research on proteolytic bacteria is still minimal. Proteolytic bacteria are a type of bacteria which can produce protease enzymes. This research was performed aiming to find selected proteolytic bacteria that can produce protease enzymes. Pour plate method using skim milk agar media was employed to isolate the bacteria. Bacteria isolates were selected based on qualitative and quantitative tests. Current research results discovered 6 bacteria isolates which had proteolytic ability after the qualitative test. Among those 6 isolates, 3 (FB2, THB1b, and THB6a) were able to hydrolyze skim milk media with the fastest incubation time of 18 hours. Meanwhile, based on quantitative test, 1 isolate produced the highest protease activity, which is FB2 isolate with 958 U/mL having a total of 12.05 cells/mL. Based on this research report, FB2 isolate collected from crocodile feces (FB) has the potential to be utilized in protease enzymes application in the environment.
Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Jendri Mamangkey; Lukas Pardosi; Riska Septia Wahyuningtyas
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Endophytic bacteria and fungi inhibite the tisues of living plant. Both of them are significant components of plant microecosystems. Current research was performed to explain several microbiological activities (production of extracellular enzymes, antioxidants, and antibacterials) of endophytic bacteria and fungi from binahong leaves extract (Anredera cordifolia (Ten.) Steenis). In this case, the research was conducted by applying a review of several literature reviews published online on Google Scholar and several journals listed on the ScienceDirect site. Based on the results obtained from the research reports from several scientific journal sources, there are four types of extracellular enzymes produced by the endophytic bacteria of the Anredera cordifolia plant, including proteinase, amylase, esterase, and cellulase. In addition, the endophytic bacteria of Pseudomonas aeruginosa from the leaves extract of Anredera cordifolia has antioxidant activity, which is volatile compounds including cis-9-Octadecenoic acid, 2-dodecenoic acid, and 9 octadecenamide produced by endophytic bacteria of Pseudomonas sp. Furthermore, the endophytic bacteria isolated from Anredera cordifolia leaves extract inhibits the growth of E. coli ATCC 35216 and S. aureus ATCC 29523. Meanwhile, the endophytic fungi from Anredera cordifolia leaves extract inhibite the growth of E.coli and S. aureus bacteria. Therefore, understanding the relationship between bacteria and endophytic fungi from the leaves extract of binahong (Anredera cordifolia (Ten.) Steenis) is essential. This knowledge can be utilized and applied well for the future production of higher quality and more medicinal plants from binahong plant (Anredera cordifolia (Ten.) Steenis).
Gambaran Kejadian Ikutan Pasca Imunisasi Covid-19 pada Mahasiswa Fakultas Kedokteran UKI Penyintas Covid-19 dan Non Penyintas Covid-19 Desy Ria Simanjuntak; Jendri Mamangkey; Nive Winda Sari Lumban Tungkup; Anggi Izdihar Mahaswari; Talitha Amalia; Bethaniel Roy Matthew
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Adverse Events Following Immunization (AEFI) is defined as 'any adverse medical event that occurs after immunization, but which does not necessarily have a cause-and-effect relationship with vaccine use. Research conducted by krammer, et al said that vaccine recipients with pre-existing immunity (seropositive) experienced systemic side effects with a much higher frequency than antibody-nave vaccines. UKI Medical Faculty student environment class 2018 and 2019. The research design used is descriptive research with a sample of primary data taken using google form as a media for filling out questionnaires with a sampling method with simple random sampling , then the data is collected and will be processed with the Microsoft program excel. The number of respondents in this study amounted to 229 respondents, consisting of 115 students of class 2018 and 114 students of class 2019. The percentage of students who were non-survivors of COVID-19 was 77.73% and survivors of COVID-19 was 27.73%. Types of vaccines 1,2, and 3 received by the two groups included Sinovac, Astrazeneca, Moderna, and Pfizer. Symptoms of AEFI in non-survivors of COVID-19 are dominated by local symptoms in the form of pain at the injection site (vaccine 1; 88.3%, vaccine 2: 92.6%, vaccine 3: 93.8%), followed by other systemic symptoms such as myalgia., fever, and headache (vaccine 1: 11.7%, vaccine 2: 7.4%, vaccine 3: 6.2%). Symptoms of AEFI in COVID-19 survivors are dominated by local symptoms in the form of pain at the injection site (vaccine 1; 69.05%, vaccine 2: 93.55%, in vaccines 3 respondents experienced mixed local and systemic symptoms. Adverse Event Following Immunization (AEFI) which dominated in both groups in the form of local symptoms such as pain at the injection site, followed by systemic symptoms such as fever, myalgia, and headache Keywords: COVID-19, COVID-19 Vaccine, Adverse Events Following Immunization (AEFI)
Gambaran Pedagogical Content Knowledge (PCK) pada Calon Guru Biologi Di Universitas Kristen Indonesia Riska Septia Wahyuningtyas; Janed Lauren Soplantila; Jendri Mamangkey
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This research aimed to determine the ability level of Pedagogical Content Knowledge (PCK) students of biology education at the Christian University of Indonesia. This research was a qualitative descriptive research. Data collection was carried out in two ways, namely by conducting a survey of respondents about pedagogic knowledge and content knowledge, then observing the learning implementation plan document which was assessed by two panelists. The population in this study were students of the Biology Education study program at the Christian University of Indonesia. The conclusion of this study was 1) The level of Pedagogical Content Knowledge (PCK) ability of biology education students was sufficient in terms of the results of written tests regarding Pedagogical Content Knowledge (PCK) knowledge, 2) Biology education students' Pedagogical Content Knowledge (PCK) ability level entry into the criteria is sufficient in terms of the results of the observation of the learning implementation plan (RPP) document
Improving Maternal Role on Toddler Oral Hygiene in Integrated Service Center (Posyandu) Beber, Cirebon, Indonesia through Participatory Approach Assistance Yenny Lisbeth Siahaan; Sekar Restuning; Ni Made Yuliana; Jendri Mamangkey
International Journal of Health and Pharmaceutical (IJHP) Vol. 1 No. 1 (2021): November 2021
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.693 KB) | DOI: 10.51601/ijhp.v1i1.5

Abstract

Poor dental health on children including caries causes pain and difficulty in chewing. This study aimed to analyze the effect of assistance model on mothers to impove toddler oral health aged 2-5 years old. This research employed quasi-experiment through pretest and posttest approach intervention. The samples were mothers having 2-5 years old toddlers who met inclusion criteria including having PHP-M teeth index score of 40, willing to be involved, the mothers have at least junior high school education level and living in the Posyandu are. Meanwhile, the exclusion criteria was mothers who rarely visited Posyandu. Furthermore, data were analyzed using univariate and bivariate. If the data were not normally distributed, then Wilcoxon test would be done. Difference obtained between before and after the assistance model was provided to the mothers with significance value of p=0.001 (p<0.05). This indicates that assistance model caused changes on mothers’ knowledge at Posyandu. Same results were obtained from attitude, skills and PHP-M index score before and after the intervention with significance value of P=0.001 (p<0.05). This shows that assistance model caused changes on attitude, skills and decrease of toddlers’ PHP-M index score at Posyandu Beber. The outcome of the current research obtained that assistance model is proven to be more effective in improving toddler oral hygiene than extension that was carried out at Posyandu.
PENERAPAN PEMBELAJARAN ONLINE DENGAN MODEL PROJECT BASED LEARNING (PBL) TERHADAP HASIL BELAJAR PESERTA DIDIK Gusniarty Simanjuntak; Riska Septia Wahyuningtyas; Jendri Mamangkey
Inteligensi : Jurnal Ilmu Pendidikan Vol 5, No 1 (2022): Inteligensi Vol.5 No.1
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/ilg.v5i1.3503

Abstract

This study was conducted to determine the application of online learning with the Project Based Learning (PBL) model on student learning outcomes. This research was classified as a quasi-experimental, with a sample of 36 students of class XI IPA 1 and IPA 2 at SMA Negeri 1 Siborongborong as the control class and the experiment through purposive sampling technique. The results show that there is an increase in the value, where previously only 65.94 increased to 82.06. In addition, further ANOVA test was also carried out with sig. 0.023 0.05 which indicates that PBL learning has an effect where the PBL model is better than conventional learning, which shows that there is a difference in the average learning outcomes of control and experimental class students. This is because the advantages of PBL are able to construct and provide opportunities to convey, listen and reflect on ideas based on individual experiences in groups so that they are able to provide meaningful learning during online learning
Bio-Priming Using Indigenous Pseudomonas Fluorescens To Improve Local Upland Rice (Padi Gogo) Production Of Paebiu Kolopua And Pae Wuna Cultivars Asri Razad; Gusti Ayu Kade Sutariati; Tresjia C Rakian; Jendri Mamangkey; Marina Silalahi
International Journal of Science, Technology & Management Vol. 2 No. 6 (2021): November 2021
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v2i5.337

Abstract

Research was performed to evaluate the effect of biopriming technique using indigenous rhizobacteria in improving local upland rice from Southeast Sulawesi. Randomized group design was applied in this research using factorial pattern consisting of 2 factors. The first factor was upland rice cultivars consisting of 2 (two) levels of Paebiu Kolopua (V1) and Pae Wuna (V2) cultivars. Meanwhile, the second factor was rhizobacteria isolates, consisting of 4 (four) levels, those are without rhizobacteria treatment (R0), KNS11 isolate (R1), KLKU02 isolate (R2) and KNW11 isolate (R3). Research results indicated that interaction between Paebiu Kolopua cultivar and KLKU02 isolate treatment significantly affected plants height with 29.22% improvement and the amount of rice per panicle with 176.33% improvement compared to the control. Therefore, KLKU02 isolate can be recommeded as the best treatment to improve the production and outcome of local upland rice plant.
Co-Authors Abdillah, Latifah Ningrum Adinugraha, Fajar Adisti Ratnapuri Adrian Hartanto, Adrian Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Andika, Vrista Andry Pratama, Andry Angela, Ezra Anggi Izdihar Mahaswari Apon Zaenal Mustopa Arfa Yanti, Nur Ario Betha Juanssilfero Aritonang, Netty Arman Harahap Aryanti Pada Soa, Christina Asri Razad Aulia Septiani Bethaniel Roy Matthew Cengiz Kayacilar Chairunnisa, Sheila Chihombori, Tatenda Calvin Dana Briggs Dian Winarty Budadana, Christyani DWI SURYANTO Eka Wulandari Ekawati, Nurlaili Elida Octavia Evi Yulinda Tpoenifu Fatimah Frans Grovy Naibaho Gulo, Joice Elma Miranda Gusniarty Simanjuntak Gusti Ayu Kade Sutariati Hibatullah, Alnadia Yusriya Hutabarat, Anne Serafin Irawan, Herman Ivan Julius Rajagukguk Jajang Gumilar Janed Lauren Soplantila Janed Lauren Soplantila Jefrino Reis Pereira Joice Elma Miranda Gulo Joko Wahyono, Daniel Kartika Sari Dewi KIKI NURTJAHJA Lilis Suryaningsih Lomo, Jhoni Yakobus Lucas William Mendes Lukas Pardosi Mada Triandala Sibero Manguntungi, Baso Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Medyawanti Pane Mustopa, Apon Mutiara, Ilma Muttaqin, Salwa Zainum Napitupulu, Agnes Ester Napitupulu, Alexis Rachel Maria Rosauli Nelius Harefa Ni Made Yuliana Nitte, Tri Maharani Permata Nive Winda Sari Lumban Tungkup Novianti, Ela Nowlinda Kamuihkar Nur Amani, Febriyanti Nurfatwa, Maritsa Octavia, Elida Ode Adi Parman Rudia, La Ode Muhamad Iman Sulaiman, La Pereira, Jefrino Reis Pilidai Endorina Nadapdap Purba, Dian Pirma Natasari Putranto, Wendry Raden Mohamad Herdian Bhakti RATIH ASMANA NINGRUM Ratnapuri, Adisti Refli, Redoyan Ria Simanjuntak, Desy Riana, Febi Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Ripaldo Sihombing Riska Septia Wahyunigtyas Riska Septia Wahyuningtyas Rivaldi Sitinjak Rosdiana Virginia Rosenta Purba, Ika Sakka, Deni Frans Salwa Zainum Muttaqin Santhya Anaomi Tamansa Sekar Restuning Sembiring, Geskia Avikasari Septiani, Aulia Serafin, Anne Setiyadi Putranto, Wendry Siahaan, Frisca Olivia Sitinjak, Rivaldi Situmorang, Agnes Feronika Sri Swasthikawati, Sri SUNARTO Sunarto Sunarto Sunarto Sunarto Talitha Amalia Tesalonika Febriani Zebua Tpoenifu, Evi Yulinda Tresjia C Rakian Tri Maharani Permata Nitte Trinugroho, Joko P Wafaretta, Erona Wahyuningtyas, Riska Septia Wijaya, Hans Yenny Lisbeth Siahaan Yudi Purnomo Zaenal, Apon Zefanya Sipahelut