Ratna Nirmala
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Induksi Multiplikasi Ubi Kayu var. Gajah (Manihot esculenta crantz) Melalui Kultur Jaringan Dengan Zat Pengatur Tumbuh BAP dan NAA Muhammad Fauzan; Ratna Nirmala; Widi Sunaryo; Penny Pujowati
Jurnal Agroekoteknologi Tropika Lembab Vol 3, No 2 (2021): Agroekoteknologi Tropika Lembab Volume 3 Nomor 2 Februari 2021
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.3.2.2021.4813.79-85

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh BAP dan NAA terhadap pertumbuhan eksplan ubi kayuvar.Gajah(Manihot esculenta crantz). Percobaan faktorial dalam rancangan acak lengkap dengan ulangan sebanyak tujuh kali digunakandalam penelitian. Faktor pertama yaitu konsentrasi NAA dengan dua taraf sebesar 0 ppm dan 0,5 ppm, sedangkan faktor kedua adalahkonsentrasi BAP dengan empat taraf sebesar 0 ppm, 0,5 ppm, 0,75 ppm dan 1 ppm. Data dianalisa menggunakan sidik ragam dandilanjutkan dengan Uji Beda Nyata Terkecil (BNT) dengan taraf 5%. Hasil penelitian menunjukkan bahwa BAP berpengaruh nyataterhadap tinggi tunas dan jumlah daun tetapi tidak berpengaruh nyata terhadap panjang akar tunas ubi kayu var. gajah. NAAberpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi tunas dan jumlah daun tunas ubi kayu var.gajah. Interaksi antara BAP dengan NAA berpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi danjumlah daun tunas ubi kayu var. gajah. Media MS dengan konsentrasi BAP: 1 ppm + NAA: 0,5 ppm merupakan konsentrasi terbaikuntuk induksi multiplikasi ubi kayu var. gajah.
Pertumbuhan dan Ketahanan Penyakit Kepok "Kuning" Pisang Paska Aklimatisasi Bibit di Pembibitan dengan Pupuk Organik Nasa Cair dan Trichoderma Ratna Nirmala; Ratna Shanti
Jurnal Agroekoteknologi Tropika Lembab Vol 1, No 2 (2019): Agroekoteknologi Tropika Lembab Volume 1 No 2 Februari 2019
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.1.2.2019.1982.77-87

Abstract

Several years ago at 2000 Kepok “Yellow” Banana plantation  in East Kalimantan was attached disease, so that the production was decrease therefor in vitro propogation was a preferred a n alternative method. The aim of this study was to knew the growth and disease resistance of Kepok “Yellow” Banana post acclimatization seedling in nursery giving trichoderma as a biological control technique. This study was conducted in April until July 2018 in Samarinda. The completely randomized factorial was used as experiemental design with two factor ie: giving trichoderma (T) consist two level : with trichoderma (T1) and without trichoderma (T0), another factor ie : giving Nasa liquid organic fertilizer (N), consist four level : N0  (0 ml/l water); N1 (2 ml/l water); N2 (4 ml/l water); N3 (6 ml/l water). So that became 8 treatments, each treatment were repeated for five times.The total population were 40 polybags. Each polybags was planted 1 seedling after post acclimatization  healthy and  strong.  The  parameter  observed    were  increase  of  plant  hight, number of leaves, length of the longest leaves, width of the widest of leaves and diameter of stem. Observation the parameter was every two weeks, except diameter of stem, wich was only the end of observation. Polybag was filled with a mixture of   top soil and goat manure with ratio 4:1. Trichoderma treatment T1 by providing trichoderma agent in mixture top soil. Treatment was done before the seedling planted in the polybag. The treatment of Nasa was done one for week. The result of research showed that the growth of banana seedling have significant different to the treatment of Nasa liquid organic fertilizer, wich was the best concentration N1 (Nasa 2 ml/liter of water. The effect of interaction giving Trichoderma and Nasa, to almost the all of growth parameter were not significant different, except in increase the length of leaves. Al trought it have tendency T1N1  the best effect to the growth of all observation. The proves that the Kepok “Yellow” Banana seedling from tissue culture were fertile growing and healthy without disease attack. Except in treatment without trichoderma on the end observation.Several years ago at 2000 Kepok “Yellow” Banana plantation  in East Kalimantan was attached disease, so that the production was decrease therefor in vitro propogation was a preferred a n alternative method. The aim of this study was to knew the growth and disease resistance of Kepok “Yellow” Banana post acclimatization seedling in nursery giving trichoderma as a biological control technique. This study was conducted in April until July 2018 in Samarinda. The completely randomized factorial was used as experiemental design with two factor ie: giving trichoderma (T) consist two level : with trichoderma (T1) and without trichoderma (T0), another factor ie : giving Nasa liquid organic fertilizer (N), consist four level : N0  (0 ml/l water); N1 (2 ml/l water); N2 (4 ml/l water); N3 (6 ml/l water). So that became 8 treatments, each treatment were repeated for five times.The total population were 40 polybags. Each polybags was planted 1 seedling after post acclimatization  healthy and  strong.  The  parameter  observed    were  increase  of  plant  hight, number of leaves, length of the longest leaves, width of the widest of leaves and diameter of stem. Observation the parameter was every two weeks, except diameter of stem, wich was only the end of observation. Polybag was filled with a mixture of   top soil and goat manure with ratio 4:1. Trichoderma treatment T1 by providing trichoderma agent in mixture top soil. Treatment was done before the seedling planted in the polybag. The treatment of Nasa was done one for week. The result of research showed that the growth of banana seedling have significant different to the treatment of Nasa liquid organic fertilizer, wich was the best concentration N1 (Nasa 2 ml/liter of water. The effect of interaction giving Trichoderma and Nasa, to almost the all of growth parameter were not significant different, except in increase the length of leaves. Al trought it have tendency T1N1  the best effect to the growth of all observation. The proves that the Kepok “Yellow” Banana seedling from tissue culture were fertile growing and healthy without disease attack. Except in treatment without trichoderma on the end observation.
Metode Mengatasi Browning pada Eksplan Ulin (Eusideroxylon zwageri) untuk Inisiasi Regenerasi Secara In Vitro trios carito; Sulistiawati sulistiawati; Ratna Nirmala
Jurnal Agroekoteknologi Tropika Lembab Vol 1, No 2 (2019): Agroekoteknologi Tropika Lembab Volume 1 No 2 Februari 2019
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.1.2.2019.1972.106-113

Abstract

Ulin (Eusideroxylon zwageri) is one of the native plants of Indonesia whose growth is spread in tropical forest, among others in southern Sumatra and Kalimantan. Based on the results of the 1998 Asia Regional Workshop meeting held at the Hanoi (Vietnam) International Union for Conservation of Nature and Natural Resources (IUCN), it was determined that ironwood is on Vulnerable A1cd + 2cd status which means sensitive or is facing a high risk of extinction. Tissue culture is a technique that needs to be applied to overcome the problem of ironwood regeneration initiation. However, the concentration of tannin with high concentration so that the formation of browning which leads to the death of ulin tissue is one of the causes of the low success of ironwood tissue culture. This study was formulated based on the potential of activated charcoal and vitamin C to overcome browning, so that it can significantly affect the growth of Ulin regeneration initiation (Eusideroxylon zwager). The analytical method used is Observation of Qualitative Parameter Treatment, namely explant color and the quantitative parameter observed is the number of explants that have browned and not browned in this case are calculated in percent. Based on the results of the research that has been done, obtained methods to overcome browning in ulin explants (Eusideroxylon zwageri) which is divided into 2 stages. In stage 1 (pre-condition) browning can be overcome with a 100% success rate, explants soaked for 24 hours in 50% MS liquid media with pH 4. In stage 2, browning can be overcome with a 100% success rate on 100% MS solid media addition of BAP 1.0 mg / L with A2B3 treatment (Vitamin C / 1 mg / L ascorbic acid, 4.00 g / L activated charcoal) placed in the dark room.
PENGARUH KONSENTRASI PUPUK ORGANIK CAIR KOSARINE TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SELADA (Lactuca Sativa L) Ratna Nirmala
Agrin Vol 17, No 2 (2013): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2013.17.2.204

Abstract

Tujuan penelitian adalah untuk mengetahui: (1) pengaruh pupuk organik cair kosarine terhadappertumbuhan dan hasil tanaman selada; dan (2) konsentrasi pupuk organik cair kosarine yang tepat dalammempengaruhi pertumbuhan dan hasil sayur selada yang terbaik. Penelitian dilaksanakan pada bulan Februarisampai April 2013 di lokasi lahan pada Jalan Kenyah Sempaja Kelurahan Sempaja Kecamatan Samarinda Utara.Rancangan percobaan yang digunakan Rancangan Acak Lengkap (RAL) terdiri atas empat perlakukankonsentrasi POC kosarine yang diulang sebanyak sembilan kali yaitu : 0 mL/L air sebagai kontrol (k0), 10 mL/Lair (k1), 20 mL/L air (k2) dan 30 mL/L air (k3). Sehingga terdapat 36 populasi tanaman yang masing-masingditanam pada polibag yang berisi tanah bekas ditanami selada, yang medianya diberi pupuk Trichokompos. Hasilpenelitian menunjukkan bahwa pengaruh berbagai konsentrasi POC kosarine terhadap pertumbuhan dan hasilsayur selada tidak memberikan perbedaan yang signifikan pada semua variabel pengamatan baik rata-rata tinggitanaman, jumlah daun, panjang daun pada umur 7, 14, 21, dan 28 hari setelah tanam (HST) maupun berat segartanaman pada saat panen. Namun cenderung pada konsentrasi 30 mL/L air (k3) pengaruh lebih baikdibandingkan pada konsentrasi yang lebih rendah terutama pada berat segar saat panen.Kata kunci : pupuk organik cair kosarine, pertumbuhan dan hasil, seladaABSTRACTAims of this research were to know the effect of several concentration of Kosarine liquid organicfertilizer on the growth and yield of lettuce (Lactuca sativa L) and to find proper concentration of kosarine forbetter growth and production of of lettuce. It was conducted at Kenyah street, Sempaja Village North Samarinda,starting from February until April 2013. This research used Randomized Completely Block Design with fourtreatments concentration of kosarine liquid organic fertilizer, consisting of 0 ml kosarine/l water as control (k0),10 ml kosarine/l water (k1), 20 ml kosarine/l water (k2), and 30 ml kosarine/l water (k3). Each treatment wasreplicated nine times, so that all treatments were 36 polybags. The polybag contained top soil mixed residuetrichocompose manure. One seedling of lettuce was planted in one polybag. Results of the research showed thatall treatments were insignificantly different in all variables of the growth and yield of lettuce like : increasingheight of plant, number of leaves, length of leaves at 7, 14, 21 and 28 days after transplanting and fresh weightof plant. Nevertheless, there was a tendency at 30 ml kosarine/l water (k3) concentration to peform better resultthan the lowest concentration on fresh weight of plant at harvest time.Key words : kosarine organic liquid fertilizer,growth and yield, lettuce