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Induksi Multiplikasi Ubi Kayu var. Gajah (Manihot esculenta crantz) Melalui Kultur Jaringan Dengan Zat Pengatur Tumbuh BAP dan NAA Muhammad Fauzan; Ratna Nirmala; Widi Sunaryo; Penny Pujowati
Jurnal Agroekoteknologi Tropika Lembab Vol 3, No 2 (2021): Agroekoteknologi Tropika Lembab Volume 3 Nomor 2 Februari 2021
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.3.2.2021.4813.79-85

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh BAP dan NAA terhadap pertumbuhan eksplan ubi kayuvar.Gajah(Manihot esculenta crantz). Percobaan faktorial dalam rancangan acak lengkap dengan ulangan sebanyak tujuh kali digunakandalam penelitian. Faktor pertama yaitu konsentrasi NAA dengan dua taraf sebesar 0 ppm dan 0,5 ppm, sedangkan faktor kedua adalahkonsentrasi BAP dengan empat taraf sebesar 0 ppm, 0,5 ppm, 0,75 ppm dan 1 ppm. Data dianalisa menggunakan sidik ragam dandilanjutkan dengan Uji Beda Nyata Terkecil (BNT) dengan taraf 5%. Hasil penelitian menunjukkan bahwa BAP berpengaruh nyataterhadap tinggi tunas dan jumlah daun tetapi tidak berpengaruh nyata terhadap panjang akar tunas ubi kayu var. gajah. NAAberpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi tunas dan jumlah daun tunas ubi kayu var.gajah. Interaksi antara BAP dengan NAA berpengaruh nyata terhadap panjang akar tetapi tidak berpengaruh nyata terhadap tinggi danjumlah daun tunas ubi kayu var. gajah. Media MS dengan konsentrasi BAP: 1 ppm + NAA: 0,5 ppm merupakan konsentrasi terbaikuntuk induksi multiplikasi ubi kayu var. gajah.
Somatic Embryos Induction of East Kalimantan Local Rice (Oryza sativa L.) Cultivars and In Vitro Selection Against Salinity Muktirianur Muktirianur; Bambang Supriyanto; Widi Sunaryo; Nurhasanah Nurhasanah
AGRIVITA, Journal of Agricultural Science Vol 44, No 2 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v0i0.2193

Abstract

Soil salinity is one major environmental constraint on rice production, especially in coastal areas. The development of salt-tolerant genotypes is considered to be the most effective breeding strategy to overcome the constraint. This study aims to induce somatic embryos formation of East Kalimantan local rice cultivars and to obtain tolerant somatic embryos under saline condition via in vitro selection. Four commonly cultivated local rice cultivars, namely Buyung, Siam, Ketalun Tawar and Serai Gunung, were used in this study. The somatic embryos were produced using three different plant growth regulator (PGR) compositions. The salinity tolerance level of somatic embryos was induced by in vitro selection in salt toxicity medium containing 0 mM; 50 mM; 100 mM; 150 mM; 200 mM NaCl. The best medium for somatic embryogenesis contains 1 mg/l 2,4-D + 0.5 m/l  BAP, resulting the highest percentage of cream and white non-compact callus on the tested cultivars. More than 70% of the somatic embryos were tolerant against salinity (NaCl 200 mM). However, only somatic embryos derived from Serai Gunung could regenerate into normal plantlets.
The diversity of banana cultivars in East Kalimantan based on morphological characteristic Sonja Verra Tinneke Lumowa; Widi Sunaryo; Reflinur Reflinur; Dora Dayu Rahma Turista; Ruqoyyah Nasution; Yaskinul Anwar; Zenia Lutfi Kurniawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 7 No 02 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i02.1672

Abstract

Banana is a plant with nutritious fruit and can help prevent stunting. Banana plants are widely cultivated in East Kalimantan, but the type characterization has not been identified. The purpose of this study was to determine the diversity of banana plants in East Kalimantan and to characterize banana plants based on morphology. This research is explorative in nature by identifying directly in the field the cultivated banana cultivars. Morphological characterization of stems, leaves and fruit was carried out based on the banana description contained in IPGRI-INIBAP/CIRAD. The research instrument is a banana morphology observation sheet which contains parameters: leaf habit, pseudostem color, predominant underlying color of the pseudostem, sap color, blotches at the petiole base, blotches color, petiole canal leaf III, color of leaf upper surface, color of midrib dorsal surface, color of leaf lower surface, color of midrib ventral surface, wax on leaves. The result shows that at East Kalimantan is found 12 types of banana cultivars were found, Talas bananas, Rutai bananas, Kepok bananas, Mas bananas, Morosebo bananas, Tanduk bananas, Susu bananas, Maulin bananas, Red bananas, Ambon bananas, Raja bananas, and Cavendish bananas.There are variations in the morphological characteristics of banana plants, leaves and fruit that can characterize each banana.
PENAMBAHAN PUPUK NITROGEN TERHADAP PERTUMBUHAN DAN HASIL SELADA (Lactuca sativa L.) PADA SISTEM HIDROPONIK SUMBU Syamad Ramayana; Penny Pujowati; Widi Sunaryo; Herdi Herdi; Helda Syahfari
Agrika Vol. 18 No. 1 (2024)
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v18i1.5824

Abstract

ABSTRAKPeningkatan hasil Selada (Lactuca sativa L.) hidroponik sistem sumbu dapat dilakukan apabila faktor-faktor pendukung pertumbuhannya terpenuhi, terutama faktor ketersediaan nutrisi tanaman. Larutan AB-mix merupakan nutrisi pada budidaya hidroponik yang menyediakan unsur hara untuk kebutuhan tanaman. Penambahan nutrisi nitrogen dari sumber lain dapat dilakukan untuk meningkatkan pertumbuhan tanaman karena meningkatkan kandungan protein yang terbentuk sehingga pertumbuhan tanaman lebih baik. Penelitian ini bertujuan mengetahui pengaruh dan konsentrasi pupuk nitrogen yang tepat dalam meningkatkan pertumbuhan dan hasil selada pada hidroponik sistem sumbu. Penelitian dilaksanakan Juni-Agustus 2023 di Desa Panca Jaya, Kecamatan Muara Kaman, Kabupaten Kutai Kartanegara. Penelitian menggunakan rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan. Perlakuan terdiri dari 5 taraf, yaitu: d0: 0 mg urea/l air; d1: 100 mg urea/l air; d2: 200 mg urea/l air; d3: 300 mg urea/l air dan d4: 400 mg urea/l air. Hasil penelitian menunjukkan bahwa penambahan pupuk nitrogen berpengaruh nyata terhadap pertambahan tinggi tanaman pada umur 7, 14, 21, 28, 35 dan 42 hari setelah pindah tanam (HSPT), pertambahan jumlah daun umur 14, 21, 28, dan 42 HSPT, berat basah tanaman, dan panjang akar. sedangkan pertambahan jumlah daun pada umur 7 dan 35 HSPT, berat basah akar, berat kering tanaman, dan berat kering akar tidak berbeda tidak nyata pada tanaman selada sistem hidroponik sumbu. Berat basah tanaman selada terbaik pada sistem hidroponik sumbu diperoleh pada konsentrasi pupuk nitrogen 200 mg nitrogen/l air; 300 mg nitrogen/l air dan 400 mg nitrogen/l air. ABSTRACTIncreasing the yield of lettuce (Lactuca sativa L.) using a hydroponic wick system can be done if the factors supporting its growth are sufficient, especially the availability of plant nutrients. AB-mix solution is nutrition for hydroponic cultivation which provides nutrients for plant needs. The addition of nitrogen nutrition from other sources can be done to increase plant growth because it increases the protein content formed so that plant growth is better. This research aims to determine the effect and appropriate concentration of nitrogen fertilizer in increasing the growth and yield of lettuce in a hydroponic wick system. The research was carried out June-August 2023 in Panca Jaya Village, Muara Kaman District, Kutai Kartanegara Regency. The study used a completely randomized design with 5 treatments and 4 replications. The treatment consisted of 5 levels, namely: d0: 0 mg urea/l water; d1: 100 mg urea/l water; d2: 200 mg urea/l water; d3: 300 mg urea/l water and d4: 400 mg urea/l water. The results showed that the addition of nitrogen fertilizer had a significant effect on increasing plant height at ages 7, 14, 21, 28, 35 and 42 HSPT, increasing the number of leaves at ages 14, 21, 28 and 42 HSPT, plant fresh weight and root length. while the increase in the number of leaves at the ages of 7 and 35 HSPT, root wet weight, plant dry weight, and root dry weight were not significantly different in lettuce plants with the wick hydroponic system. The best wet weight of lettuce plants in the wick hydroponic system was obtained at a nitrogen fertilizer concentration of 200 mg nitrogen/l water; 300 mg nitrogen/l water and 400 mg nitrogen/l water.
The diversity of banana cultivars in East Kalimantan based on morphological characteristic Sonja Verra Tinneke Lumowa; Widi Sunaryo; Reflinur; Dora Dayu Rahma Turista; Ruqoyyah Nasution; Yaskinul Anwar; Zenia Lutfi Kurniawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 02 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i02.1936

Abstract

Banana is a plant with nutritious fruit and can help prevent stunting. Banana plants are widely cultivated in East Kalimantan, but the type characterization has not been identified. The purpose of this study was to determine the diversity of banana plants in East Kalimantan and to characterize banana plants based on morphology. This research is explorative in nature by identifying directly in the field the cultivated banana cultivars. Morphological characterization of stems, leaves and fruit was carried out based on the banana description contained in IPGRI-INIBAP/CIRAD. The research instrument is a banana morphology observation sheet which contains parameters: leaf habit, pseudostem color, predominant underlying color of the pseudostem, sap color, blotches at the petiole base, blotches color, petiole canal leaf III, color of leaf upper surface, color of midrib dorsal surface, color of leaf lower surface, color of midrib ventral surface, wax on leaves. The result shows that at East Kalimantan is found 12 types of banana cultivars were found, Talas bananas, Rutai bananas, Kepok bananas, Mas bananas, Morosebo bananas, Tanduk bananas, Susu bananas, Maulin bananas, Red bananas, Ambon bananas, Raja bananas, and Cavendish bananas.There are variations in the morphological characteristics of banana plants, leaves and fruit that can characterize each banana.