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Karakteristik Kuantitatif Sapi Bali dan Sapi Simbal (Simmental X Bali) di Kecamatan Renah Pamenang Kabupaten Merangin Almakmum, Husni; Depison, Depison; Ediyanto, Helmi
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 11 No 1 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i1.132

Abstract This study aimed to determine the quantitative characteristics, the determinant of body size and shape, and the correlation of body measurements and the bodyweight of Bali and Simbal cattle in Renah Pamenang, Merangin District. The survey method was used along with purposive sampling, the samples were Bali and Simbal cattle aged I1 (Incicivus 1) and not in pregnant condition, with 60 cattle consisting of 30 males and 30 females. Observed data included: body weight, body weight gain, body length, shoulder height, chest circumference, chest weight, chest width, hip height, and canon circumference were analyzed using the t-test method. The principal component analysis was used to determine the determinant of size and shape of Bali and Simbal cattle followed by the regression and correlation analysis to determine the relationship and closeness of the relationship between body measurements with body weight. The result showed that the body weight, body weight gain, and body size of Bali cattle were significantly different (P <0.05) from Simbal. The body weight, body weight gain, and body size of Bali cattle were lower than Simbal cattle. The main characteristic of size in Bali and Simbal cattle was the Chest Circumference. The dominant characteristic of shape in Bali and Simbal cattle was shoulder height. The highest correlation between body measurements and body weight in Bali and Simbal cattle was the chest circumference. Keywords: Quantitative Characteristics; Bali Cattle; Simbal Cattle. Abstrak Penelitian ini bertujuan untuk mengetahui karakteristik kuantitatif, penciri ukuran dan bentuk tubuh, serta korelasi ukuran-ukuran tubuh dengan bobot badan sapi Bali dan Simbal di Kecamatan Renah Pamenang Kabupaten Merangin. Metode yang digunakan adalah survey dengan pengambilan sampel secara purposive sampling, yaitu sapi Bali dan Simbal, umur I1 (Incicivus 1), dan tidak dalam keadaan bunting sebanyak 60 sampel yang terdiri dari 30 ekor jantan dan 30 ekor betina pada masing-masing bangsa. Data yang dihimpun meliputi : bobot badan, pertambahan bobot badan, panjang badan, tinggi pundak, lingkar dada, dalam dada, lebar dada, tinggi pinggul dan lingkar kanon dianalisis menggunakan uji-t. Analisis komponen utama digunakan untuk mengetahui penciri ukuran dan bentuk tubuh sapi Bali dan Simbal kemudian dilanjutkan analisis regresi dan korelasi untuk mengetahui hubungan dan keeratan hubungan antara ukuran-ukuran tubuh dengan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan, pertambahan bobot badan dan ukuran-ukuran tubuh sapi Bali berbeda nyata (P<0,05) dengan Simbal. Bobot badan, pertambahan bobot badan dan ukuran-ukuran tubuh sapi Bali lebih rendah dibandingkan Simbal. Penciri ukuran pada sapi Bali dan Simbal adalah lingkar dada. penciri bentuk pada sapi Bali dan Simbal adalah tinggi pundak. Korelasi tertinggi antara ukuran-ukuran tubuh dengan bobot badan sapi Bali dan Simbal adalah lingkar dada. Kata kunci: Karakteristik kuantitatif; Sapi Bali; Sapi Simbal

Phenotype Diversity and Gene Myostatin (MSTN) of Bangkok Chicken using PCR-RFLP Triana Mardiah; Depison Depison; Helmi Ediyanto
Buletin Peternakan Vol 45, No 4 (2021): BULETIN PETERNAKAN VOL. 45 (4) NOVEMBER 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i4.68968

This study aims to characterize the phenotype and determine the diversity of the Myostatin (MSTN) gene at Bangkok chickens using the Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method. This study used 50 Bangkok chicken blood samples taken from the axillary vein on the wing. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR (polymerase chain reaction) using a pair of primers F: 5'GGT TTT GAC GAC ATG AGC CT3' R: 5'CAG GTG GAA TGT CAT GCA GA3' with product length 955 bp. Amplification products were cut using restriction enzyme MboI with cutting site ↓GATC. MSTN|MboI fragments of the Bangkok chicken were electrophoresed using 2% agarose gel and visualized using doc gel. The average difference test (T-test) on body weight and weight gain of Bangkok chickens from DOC to 3 months by gender. Polymorphism analysis includes allele frequency and genotype. Male and female Bangkok chickens have low phenotype diversity. The MSTN|MboI gene fragment is monomorphic with band positions of 492 bp, 244 bp, and 219 bp resulting in a genotype of ++, and there is one type of allele with a + allele frequency of 100%.

Karakteristik Kuantitatif Sapi Bali dan Sapi Simbal (Simmental X Bali) di Kecamatan Renah Pamenang Kabupaten Merangin: Quantitative characteristics of Bali cattle and the Simbal cattle in the Renah Pamenang Sub-district, Merangin District Husni Almakmum; Depison Depison; Helmi Ediyanto
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 11 No. 1 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i1.132

Abstract This study aimed to determine the quantitative characteristics, the determinant of body size and shape, and the correlation of body measurements and the bodyweight of Bali and Simbal cattle in Renah Pamenang, Merangin District. The survey method was used along with purposive sampling, the samples were Bali and Simbal cattle aged I1 (Incicivus 1) and not in pregnant condition, with 60 cattle consisting of 30 males and 30 females. Observed data included: body weight, body weight gain, body length, shoulder height, chest circumference, chest weight, chest width, hip height, and canon circumference were analyzed using the t-test method. The principal component analysis was used to determine the determinant of size and shape of Bali and Simbal cattle followed by the regression and correlation analysis to determine the relationship and closeness of the relationship between body measurements with body weight. The result showed that the body weight, body weight gain, and body size of Bali cattle were significantly different (P <0.05) from Simbal. The body weight, body weight gain, and body size of Bali cattle were lower than Simbal cattle. The main characteristic of size in Bali and Simbal cattle was the Chest Circumference. The dominant characteristic of shape in Bali and Simbal cattle was shoulder height. The highest correlation between body measurements and body weight in Bali and Simbal cattle was the chest circumference. Keywords: Quantitative Characteristics; Bali Cattle; Simbal Cattle. Abstrak Penelitian ini bertujuan untuk mengetahui karakteristik kuantitatif, penciri ukuran dan bentuk tubuh, serta korelasi ukuran-ukuran tubuh dengan bobot badan sapi Bali dan Simbal di Kecamatan Renah Pamenang Kabupaten Merangin. Metode yang digunakan adalah survey dengan pengambilan sampel secara purposive sampling, yaitu sapi Bali dan Simbal, umur I1 (Incicivus 1), dan tidak dalam keadaan bunting sebanyak 60 sampel yang terdiri dari 30 ekor jantan dan 30 ekor betina pada masing-masing bangsa. Data yang dihimpun meliputi : bobot badan, pertambahan bobot badan, panjang badan, tinggi pundak, lingkar dada, dalam dada, lebar dada, tinggi pinggul dan lingkar kanon dianalisis menggunakan uji-t. Analisis komponen utama digunakan untuk mengetahui penciri ukuran dan bentuk tubuh sapi Bali dan Simbal kemudian dilanjutkan analisis regresi dan korelasi untuk mengetahui hubungan dan keeratan hubungan antara ukuran-ukuran tubuh dengan bobot badan. Hasil penelitian menunjukkan bahwa bobot badan, pertambahan bobot badan dan ukuran-ukuran tubuh sapi Bali berbeda nyata (P<0,05) dengan Simbal. Bobot badan, pertambahan bobot badan dan ukuran-ukuran tubuh sapi Bali lebih rendah dibandingkan Simbal. Penciri ukuran pada sapi Bali dan Simbal adalah lingkar dada. penciri bentuk pada sapi Bali dan Simbal adalah tinggi pundak. Korelasi tertinggi antara ukuran-ukuran tubuh dengan bobot badan sapi Bali dan Simbal adalah lingkar dada. Kata kunci: Karakteristik kuantitatif; Sapi Bali; Sapi Simbal

Association of Quantitative Characteristics with Growth Hormone Gene (GH Gene) in Kerinci Duck Using PCR-RFLP Method Amalia Fildzah Ghassani; Depison Depison; Helmi Ediyanto
Buletin Peternakan Vol 46, No 4 (2022): BULETIN PETERNAKAN VOL. 46 (4) NOVEMBER 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i4.75810

This study aims to determine the association of quantitative characteristics with growth hormone gene (GH gene) in kerinci duck using PCR-RFLP method. Samples total used was 96 Kerinci ducks consisting of 43 males and 53 females and 96 blood samples. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR using a pair of primers5'-CAA GGA ACA GAG GGT TTC CA-3' and Revers : 3'-GGG AGA TAG GGC AAA CAT CA-5', with a length of product 855 bp. The amplification product was cut using restriction enzyme AluI with the AG↓CT cutting site. Growth hormone/AluI fragments of Kerinci duck were electrophoresed using 1.5% agarose gel and visualized using doc gel. Data collected includes body weight, weight gain, body measurements, and blood of Kerinci ducks. The differences in body weight, body weight gain, and body measurements, as well as differences in body weight between genotypes were tested by T-test. The determinants of the size and shape of Kerinci duck were analyzed using PCA. This study showed that the body weight, weight gain, and body sizes of male Kerinci ducks were significantly different (P<0.05) than female Kerinci ducks. The Kerinci duck GH|AluI gene is polymorphic with three genotypes, i.e, +/+ of (49%), +/- of (39.6%), and -/- of (11.5%), and two alleles, namely (+) by 69% and (–) by 31%. Conclusion: body weight, weight gain, and body sizes of male Kerinci ducks were higher than female. The size identifier of male and female Kerinci ducks were sternum, shank lengh, and shank circumference, while shape identifier was wing length. GH Genes|AluI in Kerinci duck is polymorphic. GH gene |AluI of Kerinci ducks was associated with body weight, weight gain, and body measurements, and the best is the genotype (+/+).

Polimorfisme Protein Darah Domba di Kabupaten Batanghari Silvia Erina; Helmi Ediyanto
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 15 No. 1 (2012): Mei 2012
Publisher : Fakultas Peternakan Universitas Jambi

In Pemayung and Muara Bulian Subdistrict, Batanghari District, the population of sheep is relatively high. However, information on genetic characteristics of the sheep has not been well-identified yet . Therefore, in order to reach one of the objectives of veterinary development, conservating local breeds, it is necessary to know blood protein polymorphism of the sheep. One way to identify genetic characteristic of the sheep is by analizing blood protein electrophoretically.The research is aimed at analizing genetic characteristics of sheep in Batanghari District, including characteristics of blood protein by electrophoresis to know blood protein polymorphism patterns. Blood samples were taken from 20 sheep, 10 sheep from Pemayung Subdistrict and 10 sheep from Muara Bulian Subdistrict. Analysis was conducted at Livestock Molecular Genetic Laboratory Veterinary Faculty Bogor Agriculture Institute. Allelic Frequency was directly calculated with Hardy-weinberg proportion and genetic distance is was calculated with method of Nei . The result of electrophoretic analysis has found 5 kinds of proteins ; Albumin, Postalbumin, Transferin, Posttransferin-1 and Posttransferin-2. Albumin and Postalbumin are controlled by 2 alleles ; Allele A=0,6 and allele B=0,4, Transferin is controlled by 4 alleles ; allele A = 0,025, allele B=0,50, allele C=0,45, and allele D=0,025. Posttransferin-1 and posttransferin-2 are controlled by an allele; allele A=1,00. In conclusion, there are 5 kinds of blood protein, they are Albumin, Postalbumin, Transferin, Posttransferin-1 and Posttransferin-2.Polymorphism is detected in 3 loci; Albumin, Postalbumin and Transferin with Heterozigocity Average 0,29 in Pemayung Subdistrict and 0,287 in Muara Bulian Subdistrict. Albumin and Postalbumin loci can be considered as main genetic characteristics.

Perbandingan Daya Tunas, Daya Tetas, dan Karakteristik Kuantitatif Ayam KUB dengan Ayam Kampung (G1) Utama, Millisani; Depison, Depison; Gushairiyanto, Gushairiyanto; Ediyanto, Helmi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

ABSTRAKPenelitian ini bertujuan untuk mengetahui perbandingan daya tunas, daya tetas dan karakteristik kuantitatif ayam KUB dengan ayam kampung (G1) sampai umur 3 bulan. Materi yang digunakan 315 butir telur dan 174 ekor ayam per galur dengan Metode penelitian eksperimen. Data yang dikumpulkan meliputi daya tunas, daya tetas, bobot badan, dan pertambahan bobot badan serta ukuran-ukuran tubuh. Data bobot badan, pertambahan bobot badan, dan ukuran-ukuran tubuh dianalisis menggunakan uji-t, sedangkan vektor rataan ukuran-ukuran tubuh dianalisis menggunakan uji T2-Hotelling. Penciri bentuk dan ukuran tubuh dianalisis menggunakan Analisis Komponen Utama. Hasil penelitian ini menunjukkan bahwa daya tunas ayam KUB dan ayam kampung yaitu 82,86% dan 81,59%, sedangkan daya tetas yaitu 71,26% dan 69,65%. Karakterisitik kuantitatif ayam KUB berbeda nyata p<0,05) lebih tinggi dibandingkan dengan ayam kampung. Penciri ukuran tubuh ayam KUB dan ayam kampung adalah lingkar dada, sedangkan penciri bentuk tubuh adalah panjang tubuh atas dan panjang sayap. Respon seleksi Ayam KUB dan ayam kampung yaitu 7 gr dan 9,3 gr. Kesimpulan: Ayam KUB dan ayam kampung memiliki daya tunas dan daya tetas yang relatif sama. Karakteristik kuantitatif ayam KUB lebih baik dibandingkan dengan ayam kampung. Ayam KUB dan ayam kampung memiliki respon seleksi yang positif.Keywords: Ayam KUB, ayam kampung, G1, respon seleksiComparison of Fertility, Hatchability and Quantitative Characteristics between KUB Chicken and Kampung Chicken (G1)ABSTRACTThis study aims to determine the comparison of fertility, hatchability, and quantitative characteristics of KUB chickens with kampung chickens (G1) 3 months of age. The material used is 315 eggs and 174 chickens per strain and using experimental methods. The data collected fertility, hatchability, body weight, body weight gain, and body measurements. Data on body weight, weight gain, and body measurements were analyzed using a t-test, while the mean vector of body measurements was analyzed using test T2-Hotelling. Body shape and size characteristics were analyzed using Principal Component Analysis. The results of this study showed the fertility of KUB and kampung chicken is 82.86% and 81.59%, while the hatchability is 71.26% and 69.65%. The quantitative characteristics of KUB chickens are significantly (p<0,05) higher than those of kampung chickens. The body size characteristics of KUB and kampung chickens are chest circumference, while the shape characteristics are back length and wing length. The selection response of KUB and kampung chicken is 7 g and 9.3 g. Conclusion: KUB chickens and kampung chickens have fertility and hatchability relative same. The quantitative characteristics of KUB chickens are better than of kampung chickens. KUB and kampung chickens have a positive selection response.Keywords: KUB chickens, kampung chickens, G1, selection responses

Asosiasi Karakteristik Kuantitatif Gen Hormon Pertumbuhan pada Itik Kerinci Menggunakan Metode PCR-RFLP dengan Enzim Pemotong XbaI Hidayat, Gradiandri; Ediyanto, Helmi; Depison, Depison
Jurnal Peternakan Vol 21, No 2 (2024): September 2024
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v21i2.24455

ABSTRAK. Itik kerinci merupakan salah satu plasma nutfah Provinsi Jambi yang perlu dilestarikan. Penelitian itik kerinci perlu dilakukan dalam rangka memperoleh data dasar tentang keragaman genetiknya berdasarkan karakteristik kuantitatif maupun molekuler. Tujuan penelitian ini untuk mengetahui asosiasi gen hormone pertumbuhan itik Kerinci dengan karakteristik kuantitatif menggunakan metode PCR-RFLP. Materi yang digunakan adalah itik Kerinci sebanyak 96 ekor dan sampel darah itik Kerinci sebanyak 96 sampel. Data yang dihimpun meliputi Asosiasi gen GH dengan karakteristik kuantitatif itik Kerinci menggunakan analisis (uji-t). Vektor nilai rata-rata ukuran-ukuran tubuh itik Kerinci jantan dan betina di analisis menggunakan uji T2-Hotelling. Penentu ukuran dan bentuk itik kerinci dianalisis dengan Analisis Komponen Utama. Analisis data molekuler meliputi: frekuensi genotipe dan alel, keseimbangan Hardy-Weinberg, heterozigositas, dan Polymorphic Information Content (PIC). Hasil penelitian menunjukkan bahwa bobot badan, pertambahan bobot badan, dan ukuran-ukuran tubuh itik Kerinci jantan berbeda nyata (P<0,05) lebih tinggi dibandingkan itik Kerinci betina. Hasil analisis gen GH didapatkan tiga genotip yaitu +/+ (42%), +/- (39%), dan -/- (19%), dengan dua alel yaitu (+) 62% dan (-) 38%. Populasi itik kerinci berada dalam kesetimbangan Hardy-Weinberg (P<0,05) dengan χ2 3,19. Keragaman itik Kerinci tergolong dalam tingkat sedang dengan hubungan genetik yang masih relatif jauh dengan nilai heterozigositas 0,47. Nilai PIC 0,41 yang menunjukkan bahwa primer yang digunakan cukup informatif sebagai penciri gen GH|XbaI itik kerinci. Kesimpulan: Gen GH|XbaI itik kerinci bersifat polimorfik dan memiliki asosiasi dengan bobot badan, pertambahan bobot badan dan ukuran-ukuran tubuh dengan genotipe terbaik yaitu +/+.Kata kunci: Karakteristik kuantitatif, gen hormon pertumbuhan, itik kerinci, XbaIAssociation of Quantitative Characteristics of Growth Hormone Genes in Kerinci Ducks Using PCR-RFLP Method with XbaI Cutting EnzymeABSTRACT. Kerinci duck is a germplasm of Jambi Province that needs to be preserved. Kerinci duck research must be conducted to obtain primary data on its genetic diversity based on quantitative and molecular characteristics. The aim of this study was to determine the association of Kerinci duck growth hormone genes with quantitative characteristics using the PCR-RFLP method. The material used was 96 kerinci ducks and 96 kerinci duck blood samples. Data collected include the Association of the GH gene with quantitative characteristics of Kerinci ducks using analysis (t-test). The mean value vector of body measurements of male and female kerinci ducks was analyzed using the T2-Hotelling test. Principal Component Analysis analyzed determinants of the size and shape of kerinci ducks. Molecular data analysis included: genotype and allele frequencies, Hardy-Weinberg balance, heterozygosity, and Polymorphic Information Content (PIC). The results showed that body weight, weight gain, and body measurements of male kerinci ducks were significantly different (P<0.05) higher than female kerinci ducks. The results of GH gene analysis obtained three genotypes, namely +/+ (42%), +/- (39%), and -/- (19%), with two alleles, namely (+) 62% and (-) 38%. The kerinci duck population was in Hardy-Weinberg equilibrium (P<0.05) with χ2 3.19. Kerinci duck diversity is classified as moderate, with relatively distant genetic relationships with a Heterozygosity value of 0.47. The PIC value is 0.41, which indicates that the primers used are informative enough to characterize the GH|XbaI gene of kerinci ducks. Conclusion: The GH|XbaI gene of kerinci ducks is polymorphic and has associations with body weight, weight gain, and body measurements, with the best genotype being +/+.

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