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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Media Analis Kesehatan
Nur Anita Ningsih
Universitas Nahdlatul Ulama Surabaya
Health Professions Immunology & microbiology Public Health

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STABILITAS PEMERIKSAAN HEMATOLOGI RUTIN PADA SAMPEL DARAH YANG DIDIAMKAN PADA SUHU RUANG MENGGUNAKAN CELL-DYN RUBY Gilang Nugraha; Nur Anita Ningsih; Titik Sulifah; Sitti Fitria
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.8255

Abstract

Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.
PENUNDAAN PEMERIKSAAN DIFFERENTIAL COUNT TERHADAP GAMBARAN SCATTERGRAM HEMATOLOGY ANALYZER CELL-DYN RUBY Gilang Nugraha; Nur Anita Ningsih
Jurnal Media Analis Kesehatan Vol 12, No 1 (2021): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v12i1.2036

Abstract

Dilaporkan bahwa penundaan pemeriksaan dapat mengubah morfologi leukosit pada sediaan apusan darah tepi. Cell-Dyn Ruby merupakan salah satu hematology analyzer 5-part differential dengan teknologi multiangle polarized scatter separation (MAPSS) yang membentuk scattergram WBC Differential dan scattergram NEU-EOS. Tujuan penelitian ini untuk mendapatkan gambaran scattergram diff count Cell-Dyn Ruby pada bahan pemeriksaan yang ditunda. Penelitian menggunakan desain Experimental Laboratory, pemeriksaan menggunakan hematology analyzer Cell-Dyn Ruby. Pemeriksaan 0 jam sebagai kontrol, pemeriksaan 6, 12 dan 24 jam sebagai perlakuan. Sebanyak 30 responden berpartisipasi dalam penelitian ini, gambaran scattergram setelah penundaan berupa peningkatan jumlah titik pada warna hitam dan merah muda, serta adanya pelebaran distribusi titik warna oranye. Berdasarkan uji statistik, terdapat perbedaan hasil pemeriksaan secara signifikan (P < 0,01) pada seluruh parameter pemeriksaan leukosit yang terdiri dari white blood cell (WBC), neutrofil segmen (SEG), neutrofil batang (BAND), immature granulocytes (IG), monosit (MONO), eosinofil (EO), basofil (BASO) dan limfosit (LYM). Hasil penelitian kami menunjukkan bahwa penundaan pemeriksaan dapat terdeteksi oleh scattergram Cell-Dyn Ruby khususnya channel WBC Differential setelah penundaan 12 jam, dan terlihat secara nyata setelah penundaan 24 jam. Saran penelitian ini adalah pemanfaatan scattergram untuk tindakan verifikasi kelayakan bahan pemeriksaan dan perlunya ada penelitian lanjutan untuk validasi dengan hapusan darah serta adanya komparasi dengan alat lain yang memiliki teknologi berbeda.Kata kunci : Cell-Dyn Ruby, diff count, penundaan, scattergram
Co-Authors Gilang Nugraha Sitti Fitria Titik Sulifah