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PENETUAN FORMULA UNTUK MENETAPKAN ESTIMASI KOLESTEROL-LDL Nugraha, Gilang; Edijanto, Soebagijoh Poegoeh
Jurnal Ilmu Kesehatan dan Kesehatan Vol 2 No 2 (2018): AUGUST
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v2i2.587

Abstract

Abstract: Measurement LDL-Cholesterol (LDL-C) can be performed by estimating LDL-C (cLDL-C) inthe blood. In addition to the first proposed Friedewald formula, several new formulas that promise betterexamination in determining LDL-C levels, proposed by Anandaraja, Puavilai, Chen, Vujovic, deCordova and Dansethakul. The subjects of the study were patients who performed routine lipid profileexamination in Institution of Clinical Pathology of Gedung Pusat Diagnostik Terpadu RSUD Dr.Soetomo Surabaya who performs 10 to 12 hours of fasting with TG less than 400 mg / dL. The sampleconsisted of 41 men and 48 women with an average age of 52 years. The statistical test on each cLDLC gave the Friedewald -1.32% bias value; Anandaraja -3,92%; Puavilai 4.26%, Chen -2.19%; Vujovic-23.65%; de Cordova -0.91% and Dansethakul 4.45%. The correlation coefficient on Friedewald0.9509; Anandaraja 0.9013; Puavilai 0.9576, Chen 0.9585; Vujovic 0.8745; de Cordova 0.9300 andDansethakul 0.9505. The proposed cLDL-C formula Chen et al promises in determining the LDL-Cestimate and the Vujovic formula gives a poor result in this study. Validation methods should be appliedto each of the cLDL formulas if they are to be applied to clinical laboratories.
PEMERIKSAAN LABORATORIUM UNTUK DETEKSI DINI PENYAKIT PADA LANSIA DI KARANGREJO SURABAYA Nugraha, Gilang; Hanik, Umi; Sumarsono, Thomas
JURNAL PENGEMBANGAN KOMUNITAS Vol 1 No 1 (2017): July
Publisher : JURNAL PENGEMBANGAN KOMUNITAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.237 KB)

Abstract

Sebagian besar kematian lansia di negara berkembang disebabkan oleh penyakit regeneratif, penyakit tersebutmerupakan penyakit kronis yang sering diderita lansia dan menyebabkan ketidakmampuan (disabilitas) seperti penyakitjantung koroner, stroke, diabetes, kanker dan penyakit paru-paru. Sehingga penyakit tersebut sangat mengganggu bagilansia karena menurunkan kualitas hidup, sehingga perlu dilakukan pemeriksaan kesehatan rutin guna memantaumunculnya penyakit regeneratif. Pemeriksaan laboratorium merupakan salah satu pemeriksaan penunjang dalammendeteksi secara dini kemunculan penyakit kronis pada lansia. Dengan kegiatan Pengabdian pada Masyarakat,diharapkan dapat meningkatkan kesadaran pada masyarakat tentang pentingnya pemeriksaan kesehatan rutin khususnyapemeriksaan laboratorium kesehatan guna memantau kemunculan penyakit regeneratif. penyuluhan pemeriksaanlaboratorium untuk deteksi dini penyakit pada lansia dan mencegah kerusakan gigi dan jaringan penyangga sertapemeriksaan urine rutin memberikan wawasan bagi masyarakat tentang kebersihan mulut dan peran laboratorium dalammemantau kesehatan pada lansia dari penyakit degeneratif serta memantau kesehatan melalui urin rutin.Kata Kunci : penyakit pada lansia, penyakit regeneratif, kerusakan gigi
Pemeriksaan Kadar Hemoglobin dengan Metode POCT (Point of Care Testing) sebagai Deteksi Dini Penyakit Anemia Bagi Masyarakat Desa Sumbersono, Mojokerto Nidianti, Ersalina; Nugraha, Gilang; Aulia, Ilmiah Alvi Nisa; Syadzila, Saumia Khoirunnisa; Suciati, Sri Surya; Utami, Nila Dwi
Jurnal Surya Masyarakat Vol 2, No 1 (2019): November 2019
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.2.1.2019.29-34

Abstract

Kasus anemia di Indonesia masih cukup tinggi hasil menunjukkan bahwa angka prevalensi anemia secara nasional pada semua kelompok umur adalah 21,70 %. Sedangkan prevalensi anemia di Provinsi Jawa Timur sebesar 5,8 %. Anemia adalah kondisi penyakit dimana jumlah sel darah merah lebih rendah dari jumlah normal. Kondisi ini biasa disebut sebagai kekurangan darah. Anemia dapat dideteksi dengan melakukan pengukuran kadar hemoglobin (Hb) menggunakan metode POCT (Point of Care Testing). Metode POCT merupakan metode yang dilakukan untuk pemeriksaan sederhana dengan menggunakan sampel dalam jumlah sedikit, mudah, cepat serta efektif dilakukan di daerah-daerah dengan jumlah fasilitas kesehatan seperti pusat kesehatan masyarakat (puskesmas), rumah sakit yang relatif sedikit khususnya di lokasi pengabdian masyarakat di Desa Sumbersono, Mojokerto. Jenis metode yang digunakan adalah observasional analitik. Pengabdian masyarakat ini bertujuan untuk skrining (pemeriksaan) kadar hemoglobin sebagai deteksi dini penyakit anemia dengan metode POCT, mengetahui hubungan antara umur, pendidikan, pekerjaan terhadap kejadian anemia serta sebagai upaya preventif (pencegahan) untuk meminimalisir penyakit anemia. Hasil menunjukkan dari 48 reponden (laki-laki 11 orang), (perempuan 37 orang) bahwa kejadian anemia pada perempuan 40 % lebih besar dibandingkan dengan laki-laki 6 %. Jumlah  kasus anemia terbanyak terjadi pada umur ≥ 60 presentase 36,36 % dibandingkan kelompok umur lainnya. Jumlah kejadian anemia terbanyak dengan presentase 46 % pekerjaan Petani dibandingkan pekerjaan lainnya. Jumlah anemia dengan pendidikan sekolah dasar presentase 16,67 % terbanyak dibandingkan dengan pendidikan lainnya. Kesimpulannya tidak terdapat hubungan antara umur, pendidikan, pekerjaan terhadap penyakit anemia bagi masyarakat desa Sumbersono, Mojokerto.Kata kunci: Anemia, Kadar Hemoglobin . POCT, Faktor AnemiaAbstract Cases of anemia in Indonesia are still quite high. The results show that the national prevalence of anemia in all age group is 21.70%. While the prevalence of anemia in East Java Province was 5.8%. Anemia is a disease because the number of red blood cells is lower than the normal number. This condition is commonly referred to blood deficiency. Anemia can be detected by measuring of hemoglobin (Hb) levels using the POCT (Point of Care Testing) method. The POCT method is a method that is carried out for a simple examination using a small samples, easy, fast and effective carried out in areas with a number of health facilities such as community health centers, hospitals that are relatively small, especially in community service locations in the village Sumbersono, Mojokerto. The type of method used is analytic observational. This community service activities aims to screening (examining) hemoglobin levels as an early detection of anemia using the POCT method, to find out the relationship between age, education, work and anemic events and as a preventive measure (prevention) to minimize anemia. The results showed there were 48 population, while (11 men), (37 women) that the incidence of anemia in women was 40% greater than 6% men. The highest number of anemia cases occurred at age ≥ 60 is 36.36% compared to other age groups. The highest number of anemia occurrences with 46% of Farmers' work compared to other occupations. The highest number of anemia with primary school education level is 16.67% compared to other education levels. Conclution There was no significant correlation between age, educational background, works and anemia cases in community the Sumbersono village, Mojokerto.
Hubungan Antara Karakteristik dengan Kadar Ferritin pada Ibu Hamil Trimester III di Puskesmas Jagir Surabaya Nur Masruroh; Gilang Nugraha
Jurnal Sehat Mandiri Vol 15 No 2 (2020): Jurnal Sehat Mandiri, Volume 15, No.2 Desember 2020
Publisher : Poltekkes Kemenkes Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.667 KB) | DOI: 10.33761/jsm.v15i2.200

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Ferritin is an iron storage protein and is extracellular in serum. Ferritin serum functions as a clinical marker of the body's iron stores status. Low ferritin levels illustrate the low iron stores that lead to iron deficiency conditions resulting in anemia in pregnant women. Some maternal characteristics affect ferritin levels in pregnant women.This study aims to determine the relationship between characteristics with ferritin levels. Taking the location of the research at the Jagir Public Health Center in Surabaya with a sample of 30 respondents who met the inclusion and exclusion criteria. The research method was observational analytic by taking maternal venous blood to be examined for ferritin levels then looking for the relationship with the characteristics of pregnant women and analyzed using chi square. More than half (83%) of respondents had ferritin levels <30 µg / ml. The results of the analysis using chi square (p = <0.05) show that there is a relationship between age (0.04) and parity (0.01) with ferritin levels. No relationship between education (0.71) and employment (0.23) with ferritin levels. Age and parity were related to ferritin levels. Education and work are not related to ferritin levels for pregnant women in the third trimester. It is expected that pregnant women increase consumption of foods that contain lots of iron to prevent anemia and health workers provide socialization of foods that contain lots of iron.
Comparative Test of Ret-He Examination in Diagnosis of Iron Deficiency in Pregnant Women Gilang Nugraha; Nur Masruroh; Diyan Wahyu Kurniasari
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (821.795 KB) | DOI: 10.31964/mltj.v6i2.303

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The most common cause of anemia is iron deficiency, which found in 50% of anemia cases. There are 66.7% of pregnant women in Indonesia that still lack iron intake. Reticulocyte hemoglobin equivalent (Ret-He) examination as a single parameter is the initial indicator of iron deficiency, considered more sensitive in detecting iron reserves in the body. This study aims to determine the effectivity of the Ret-He examination as a single parameter in establishing the diagnosis of iron deficiency in pregnant women. This research was conducted on 30 pregnant women. The sensitivity, specificity, and predictive value of Ret-He were determined by comparing the results of the biochemical examination of iron profiles. Iron deficiency defined as an abnormal examination result on two or more biochemical parameters, which were SI 425 µg/dL, serum ferritin
PENGARUH NORMAL FLORA Streptococcus sp. KARANG GIGI TERHADAP PEMERIKSAAN DARAH LENGKAP PADA MAHASISWA UNIVERSITAS NAHDLATUL ULAMA SURABAYA Rahayu Anggraini; Umi Hanik; Gilang Nugraha; Dwi Lestari Pertiwi
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 1 No 1 (2017): Medical Technology and Public Health Journal March 2017
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v1i1.757

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This study aims at determining the normal flora of Streptococcus sp., the inhabitants of the oral cavity in the tartar that can lead to health problems. This study is an observational study with cross sectional analytic approach performed on 47 samples of dental smear for Streptococcus sp. bacteria identification and EDTA blood samples for Hemoglobin level examination, hematocrit percent, the number of erythrocytes, leukocytes and platelets in University of Nahdlatul Ulama Surabaya students. The result of Independent Samples T Test data analysis in a cut point showed that the levels of hemoglobin, number of leukocytes and platelets look significantly different with p <0.05in individuals with normal flora Streptococcus alpha and Streptococcus gamma. The conclusion in this study, in individuals with normal flora Streptococcus alpha, can decrease hemoglobin level, whereas in individuals with normal flora Streptococcus gamma can increase the number of leukocytes and decrease the number of platelets.
PENGARUH PENINGKATAN LAJU ENDAP DARAH TERHADAP HASIL PEMERIKSAAN FERITIN SERUM PADA IBU HAMIL gilang nugraha; Nur Masruroh
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 3 No 2 (2019): Medical Technology and Public Health Journal September 2019
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v3i2.1133

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Serum ferritin (SF) examination is a test parameter used to assess iron deposits in the body. SF examination has limitations, the degree is influenced by inflammation because ferritin is an acute phase protein. Elevated SF levels follow the increase in other acute phase proteins, the acute phase proteins of the gamma-globulin group to increase Erythrocyte Sedimentation Rate (ESR), as the proteins neutralize the membrane of the negatively charged erythrocytes Thus accelerating the ESR. Enhanced ESR can also be found in pregnant women, the increase in such ESR occurs during pregnancy. This research aims to determine whether there is an increase in ESR relation to SF examination results in pregnant women. The study used Cross sectional studies conducted in Puskesmas Jagir, Wonokromo, Surabaya City which was conducted in May 2019, with the samples of pregnant women research. Blood sampling is 6 mL and is accommodated as much as 3mL on purple tubes for ESR inspection and 3mL on red tubes for SF examination. Laboratory test results are conducted in correlation tests. A total of 30 pregnant women respondents participated in the study, an average age of 27 years and an average gestational age of 34 weeks. ESR inspection results are 56 mm/hr (range: 27 – 117 mm/hr) and SF 20 ng/mL test result (range: 2 – 149 ng/mL). The correlation test result indicates that there is no increase in the ESR value of the SF level (P = 0.240). It was concluded that the increase in ESR in pregnant women did not affect the results of SF examinations, increased due to hemodilution due to the physiological factor of pregnancy, resulting in decreased blood viscosity and increased blood erythrocyte sedimentation rate.
Pengaruh Lama Perawatan Terhadap Indeks Platelet Pasien Demam Berdarah Dengue (Dbd) Di Rumah Sakit Islam Jemursari Surabaya Ruri Arinil Khoiro; Gilang Nugraha; Wieke Sriwulan
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 5 No 1 (2021): Medical Technology and Public Health Journal March 2021
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v5i1.1150

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Dengue Hemorrhagic Fever (DHF) is one of the most tropical infectious diseases in Indonesia caused by dengue virus and mediated by the bites of Aedes aegypti and Aedes albopictus mosquitoes. One of the clinical symptoms of dengue disease is a decrease in the number of blood platelets. There are several platelet indices that are known as indicators of platelet activation, namely Mean Platelet Volume (MPV), and Platelet Distribution Width (PDW). DHF patients who experience treatment at the hospital only monitor the low platelet count in healing. The purpose of this study was to determine the effect of length of treatment on the MPV Platelet Index and PDW of DHF patients. Data was obtained by taking primary data at Jemursari Islamic Hospital Surabaya. This research is an One Shot Case Studies research with description analitic method and Cross sectional design in DHF patients at Jemursari Islamic Hospital Surabaya. The sampling technique uses accidental sampling technique. Analysis of MPV and PDW data using the Wilcoxon Sign Rank Test non-parametric test. The test was used to see the effect of patient length of care on the MPV and PDW platelet index with a significance level of α = 0.05. The results of the Wilcoxon Signk Rank Test on the MPV show a significance of (ρ = 0.845), and the results of the Wilcoxon Signk Rank Test statistic on the PDW show a significance of (ρ = 0.469). Based on the results of the study, it can be concluded that there is no effect on the length of treatment on the MPV and PDW Platelet Index in DHF patients at Jemursari Hospital Surabaya.
SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS Gilang Nugraha; Rohayati Rohayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.913

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Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE Noor Febryani; Ikke Nanda Amalia; Intan Dwi Anggraeni; Gilang Nugraha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1311

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Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test.
Co-Authors Abdul Muhith Abraham Ahmad Ali Firdaus Ahmad Jazuly Nabil Akhmad Rizal Effendi Alfia, Shofa Alya Salsabila Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Ariska Widya Arsya Tazkiya Asror, Anita Rizki Rahmawati Atika Nur Laila Aulia, Ilmiah Alvi Nisa Ayu Slatim Maifanda Ballihgoo, Hamiduumajid Ballihg Bermawi, Bastiana Brina Thursina Dibiasi Budiarti, Rina Devyana Dyah Wulandari Diyan Wahyu Kurniasari Dwi Handayani Dwi Lestari Pertiwi Dwijayanti, ira Edhi Rianto Edijanto, Soebagijo Poegoeh Edijanto, Subagijo Poegoeh Edjianto, Soebagijo Poegoeh Effendi, Akhmad Rizal Ersalina Nidianti Fakurazi, Sharida Fauziah, Luqiana Hakim, Muhammad Yusuf Ramadhani Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Hidayatih, Nurul Ikke Nanda Amalia Ilmiah Alvi Nisa Aulia Intan Dwi Anggraeni Ira Dwijayanti Janetra Yudhis Nata P Kurniasari, Diyan Wahyu Laila, Atika Nur Layla Agustina, Nurul Ivada Maghfuroh, Qonita Iklila Maritza, Phasa Shoofii Luthf Marselli Widya Lestari Maulana Bagas Syaifulloh Mirshall Abiedama Prayoga Moch. Sahri Mohammad Nasir Muhamad Damar Baskara Muhammad Afwan Romdloni Muhammad Nasir Mukarramah, Imraatul Murdiningsih, Eka Indah Nastasya Nunki Nathalya Dwi Kartika Sari Navis, Khiliah Nila Dwi Utami Noor Febryani Nugraha, Dayu Satria Nur Anita Ningsih Nur Masruroh Nur Masruroh Nur Masruroh, Nur Nur Muhamad Nuzulul Syufi Nur Zalsabila Isnaini Prayekti, Endah Putri, Dwi Yuniar Nandita Rachmayanti, Notrisia Rahayu Anggraini Rahayu, Mita Rahmawati, Lili Susan Rizka Wahyu Safitri Rizki Nurmalya Kardina Rohayati Rohayati Rosa Sastrawati Rosda Rodhiyana Rodhiyana Ruri Arinil Khoiro S. P. Edijanto Penulis Salimi, Syafrina Maulidyatus Salman Firmansyah Salsabila, Alya Salsabillah Karunia Sugiarto Saumia Khoirunnisa Syadzila Siegfried Syafier, Siegfried Siti Husnul Khotimah Siti Rosa Lina Sitti Fitria Sri Surya Suciati Subagijo Poegoeh Edijanto Suciati, Sri Surya Sumarsono, Thomas Syadzila, Saumia Khoirunnisa Syinta Nur Isnaini Thomas Sumarsono Titik Sulifah Umi Hanik Utami, Nila Dwi Widya Novita Sari Wieke Sriwulan Wijanarko, Robert Aditya Wulan, Wieke Sri Zalfa, Anisatuz