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Isolasi Bakteri Asam Laktat dari Bekasam Ikan Patin dan Potensi Antimikrobanya terhadap Beberapa Bakteri Patogen Retno Tri astuti; Hefti Salis Yufidasari; Happy Nursyam; Jessica Della G.B
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 3 (2021): JFMR VOL 5 NO.3
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.03.10

Abstract

Bekasam merupakan produk fermentasi spontan asal Indonesia yang terbuat dari ikan air tawar dengan tambahan garam dan sumber karbohidrat. Salah satu jenis bakteri yang tumbuh pada bekasam dan menentukan keberhasilan proses fermentasinya adalah bakteri asam laktat. Bakteri asam laktat memproduksi berbagai senyawa yang berperan dalam pembentukan rasa dan aroma produk serta mencegah kerusakan/pembusukan produk. Penelitian ini dilakukan dengan tujuan untuk melakukan isolasi bakteri asam laktat asal bekasam serta potensinya sebagai antibakteri terhadap bakteri patogen pangan. Penelitian ini bertujuan untuk melakukan isolasi bakteri asam laktat asal bekasam ikan patin dan menguji aktivitas antagonisnya terhadap E. coli, Salmonella dan S. aureus. Pengujian aktivitas antimikroba dilakukan dengan metode double layer pada media MRSA. Hasil uji antibakteri menunjukkan sebanyak 58,3%, 66,67% dan 91,67% total BAL memiliki aktivitas antibakteri terhadap E. coli, Salmonella dan S. aureus. Tiga isolat BAL dengan aktivitas tertinggi berturut-turut adalah isolat HRB.1.10; HRB.1.7; dan HRB.1.11. Ketiga isolat BAL tersebut merupakan bakteri gram positif, tidak memiliki aktivitas katalase dan tidak menghasilkan gas pada media mengandung glukosa.
Characteristics and Use of Peptones from Catfish (Clarias gariepinus) and Pangas Catfish (Pangasius pangasius) Heads as Bacterial Growth Media Dwi Setijawati; Abdul Aziz Jaziri; Hefti Salis Yufidasari; Mohammad Dwi Pratomo; Dian Wahyu Wardani; Dinda Ersyah; Nurul Huda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 1 (2020): May 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.789 KB) | DOI: 10.15578/squalen.v15i1.437

Abstract

Peptone is a hydrolysate product rich in amino acids, and it is uncoagulated at high temperature. Commercial peptone produced from land animals cannot be declared as acceptable in terms of lawfulness due to religious concerns. Catfish (Clarias gariepinus) and pangas catfish (Pangasius pangasius) are important species for the fish processing industry in Indonesia. The filleting process resulted in value by-products. The fish head as the by-products can be utilized as a main raw material for higher economic value products, such as peptone. The aim of this study was to characterize peptones extracted from the heads of catfish and pangas catfish with different acid conditions. The characteristics of chemical composition, yield, color parameter, solubility, amino acid content, bacterial growth rate and biomass production were observed. The catfish peptone (CFP) and pangas catfish peptone (PCP) obtained with different acid conditions showed high protein content in the range of 84.35% to 90.80% (P0.05). The yields of CFP and PCP were significantly different (P0.05) and varied between 4.75% and 5.66%. The solubility of treated peptones varied between 98.03% and 99.52%, and the peptones were rich in glycine, glutamic acid, proline and leucine. Bacterial growth test showed that both CFP and PCP had better growth rates compared to the commercial peptone tested in this study. In addition, the biomass production with peptone from catfish and pangas catfish was higher than that with the commercial product (P0.05). This research proposed that catfish and pangas catfish heads could be developed as an alternative source for peptone production.