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All Journal Jurnal Sain Veteriner Jurnal Kedokteran Hewan
Dwi Endrawati
Departemen Klinik, Reproduksi dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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EVALUATION OF SULFURIC ACID, BARIUM CHLORIDE, AND SEED GERMINATION ASSAY METHODS AS EARLY PREGNANCY DETECTION INSTRUMENTS IN CATTLE Azmi, Zul; Desem, Muhammad I.; S. Purba, Hastuti Handayani; Endrawati, Dwi; Rachmawati, Faidah; Kusumaningtyas, Eni; Subekti, Didik T.
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.15271

Abstract

This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.
IDENTIFIKASI GOLONGAN DARAH DAN KEMUNGKINAN HUBUNGANNYA DENGAN WARNA RAMBUT PADA KUCING DOMESTIK INDONESIA (Felis familiaris) g, Gunanti; Endrawati, Dwi; Supriadi, Helny Rosita; Siswandi, Riki; Agungpriyono, Srihadi
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.571

Abstract

Penelitian ini bertujuan mengetahui distribusi penyebaran golongan darah A, B, dan AB dan mengetahui kemungkinan hubungan antaragolongan darah dengan kemunculan pola warna rambut pada kucing domestik di Indonesia. Pada penelitian ini digunakan 50 ekor kucing domestik yang berasal dari daerah Bogor dan Bekasi. Pemeriksaan golongan darah kucing dilakukan dengan identifikasi golongan darah dengan kit antibodi monoklonal Shigeta(Jepang). Hasil penelitian menunjukkan bahwa semua kucing domestik yang diteliti bergolongan darah A dan tidak diperoleh tanda-tanda adanya hubungan antara golongan darah dengan warna rambut. Terdapat sembilan kemunculan warna rambut yang terdiri atas warna tunggal, kombinasi dua warna, dan kombinasi tiga warna.
Identification of Bovine Rotavirus Group A in Bogor, West Java Hewajuli, Dyah Ayu; Pratama, Yuda; Winarsongko, Agus; Purwani, Ani; Suyatno, Teguh; Fabeane, Ajeng; Ermayati, Ermayati; Nuradji, Harimurti; Assadah, Nur Sabiq; Endrawati, Dwi; Ratnawati, Atik; Saepulloh, Muharram; Dharmayanti, NLP Indi
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90683

Abstract

Abstrak  Diare merupakan penyakit yang menyebabkan angka kesakitan yang tinggi pada pedet dan kematian neonatal. Penyakit ini dapat disebabkan oleh beberapa agen penyakit yang berbeda. Rotavirus Grup A (RVA) atau Bovine Rotavirus merupakan salah satu agen infeksi penyebab diare pada pedet. Selanjutnya, diare neonatal pada pedet dapat berdampak pada kerugian ekonomi bagi ternak sapi perah dan sapi potong di seluruh dunia karena menyebabkan gangguan pertumbuhan, meningkatnya biaya perawatan, dan/atau kematian pada hewan sakit. Prevalensi Bovine Rotavirus dapat berbeda antar negara di seluruh dunia. Sirkulasi Bovine Rotavirus pada sapi telah dilaporkan di beberapa negara tetapi sirkulasi Bovine Rotavirus pada sapi di Indonesia belum diketahui. Untuk mengetahui prevalensi Rotavirus grup A atau Bovine Rotavirus (BRV), 100 sampel feses dikoleksi dari pedet dengan gejala klinis diare atau tidak diare di Kabupaten Bogor, Jawa Barat pada tahun 2021. Sampel dianalisis terhadap urutan yang mengkode protein kapsid bagian dalam VP6 (subkelompok) menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Lima dari 100 sampel feses sapi (5%) terdeteksi positif BRV. Pada penelitian ini menunjukkan bahwa kelompok Rotavirus atau Bovine Rotavirus (BRV) telah bersirkulasi di antara ternak sapi di Indonesia, khususnya Kabupaten Bogor. Sampel positif  Rotavirus grup A atau Bovine Rotavirus (BRV) dapat diidentifikasi dengan metode diagnosis dini (RT-PCR). Kata kunci : Bovine Rotavirus; RT PCR; Bogor Abstract              Diarrhea is the most disease that cause high morbidity in calves and neonatal mortality. This disease can be caused by several different infectious agents. Group A rotaviruses (RVA) or Bovine Rotavirus are one of the infectious agents causing diarrhea in calves. Then, Neonatal calf diarrhea can impact to economic losses to dairy and beef cattle herds worldwide, in consequence of growth disorders, value of treatment, and/or death of sick animals. The prevalence of Bovine Rotavirus can become different in the worldwide. The circulation of these bovine rotavirus in calves from the some region has already been demonstrated but the circulation of bovine rotavirus in Indonesia is not known. To investigate the prevalence of A group Rotaviruses or Bovine Rotavirus (BRV), 100 fecal samples were collected from calves with diarrhea or no diarrhea in Bogor district, West Java at 2021. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) using RT-PCR. Five of 100 specimens of bovine fecal (5%) were detected positive as BRV positive. In this study, A group Rotaviruses or Bovine Rotavirus (BRV) have been circulated among cattle herds in Indonesia, particularly Bogor District. The positive samples of A group Rotaviruses or Bovine Rotavirus (BRV) can be identified using the early diagnosis method (RT-PCR). Keywords : Bovine Rotavirus; RT PCR; Bogor District
Co-Authors Assadah, Nur Sabiq Atik Ratnawati Azmi, Zul Desem, Muhammad I. Didik T. Subekti Dyah Ayu Hewajuli Eni Kusumaningtyas Ermayati, Ermayati Fabeane, Ajeng Gunanti . Helny Rosita Supriadi NLP Indi Dharmayanti Nuradji, Harimurti Prima Astuti Handayani Purwani, Ani Rachmawati, Faidah Riki Siswandi Saepulloh, Muharram Srihadi Agungpriyono Suyatno, Teguh Winarsongko, Agus yuda pratama