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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences

Eni Kusumaningtyas

1) Balai Besar Penelitian Veteriner, Jl RE Martadinata 30 Bogor, Bogor 2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor

Author-ID : 303215

Veterinary

Published : 4 Documents Claim Missing Document

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Articles

The Role Of Milk Peptide As Antimicrobial Agent In Supporting Health Status Kusumaningtyas, Eni
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 2 (2013)
Publisher : Indonesian Animal Sciences Society

Antimicrobial peptide is commonly present in all species as a component of their innate immune defense against infection. Antimicrobial peptides derived from milk such as isracidin, casocidin, casecidin and other fragments with variety of amino acid sequence are released upon enzymatic hydrolysis from milk protein Ð-casein, Î±-casein, Î²-casein, Î±-lactalbumin and Î²- lactoglobulin. These peptides were produced by the activity of digestive or microbial protease such as trypsin, pepsin, chymosin or alcalase. The mode of action of these peptides is by interaction of their positive with negative charge of target cell membrane leading to disruption of membrane associated with physiological event such as cell division or translocation of peptide across the membrane to interact with cytoplasmic target. Modification of charged or nonpolar aliphatic residues within peptides can enhance or reduce the activities of the peptides against a number of microbial strains and it seems to be strain dependent. Several peptides act not only as an antimicrobial but also as an angiotensin-converting enzyme inhibitor, antioxidant, immunomodulator, antiinflamation, food and feed preservative. Although the commercial production of these peptides is still limited due to lack of suitable large-scale technologies, fast development of some methods for peptide production will hopefully increase the possibility for mass production. Â Key words: Peptide, antimicrobial, milk

Local isolate of Saccharomyces cerevisiae as biocompetitive agent of Aspergillus flavus Kusumaningtyas, Eni
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Aspergillus flavus is a toxigenic fungus that contaminates feed and influences the animal health. Saccharomyces cerevisiae can be used as a biocompetitive agent to control the contamination. The ability of local isolate of S. cerevisiae as a biocompetitive agent for A. flavus was evaluated. A. flavus (30ml) was swept on Sabouraud dextrose agar (SDA), while S. cerevisiae was swept on its left and right. Plates were incubated at 28oC for nine days. Lytic activity of S. cerevisiae was detected by pouring its suspension on the centre of the cross streaks of A. flavus. Plates were incubated at 28oC for five days. Growth inhibition of A. flavus by S. cerevisiae was determined by mixing the two fungi on Potato dextrose broth and incubated at 28oC for 24 hours. Total colony of A. flavus were then observed at incubation time ofÂ 2, 4, 6 and 24 hours by pour plates method on the SDA plates and incubated on 28oC for two days. Growth of hyphae of A. flavus sweep were inhibited with the swept of S. cerevisiae. The width of A. flavus colony treated with S. cerevisiae is narrower (3,02 cm) than that of control ( 4,60 cm). The growth of A. flavus was also inhibited on the centre of cross streak where the S. cerevisiae poured. S. cerevisiae gradually reduced the colony number of A. flavus in the mixed culture of broth fungi ie. 14 x 103 CFU/ml while colony number of control is 80 x 103 CFU/ml. Results showed that S. cerevisiae could be used as biocompetitive agent of A. flavus. Key Words: Aspergillus flavus, Saccharomyces cerevisiae, Biocompetitive agent

Evaluation of group of Alpinia galanga n-hexane-Extract against Candida albicans by bioautography and thin layer chromatography Kusumaningtyas, Eni; Sukmawati, Lusi; Astuti, Estie
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Alpinia galanga has been used for centuries as a remedy for human diseases because it contains of therapeutic compounds. The objectives of this study was to define groups of the antifungal compounds of Alpinia galangal n-hexane-extract. Alpinia galanga was extracted by maceration method and the compounds were analyzed by phytochemical analysis. The extract was run on the thin layer chromatography (TLC) plate silica gel GF254 with dichloromethane and toluene. Bioautography was conducted to determine antifungal compounds against Candida albicans. Active compounds on the previous step were identified by running extract on TLC plate and sprayed with Vanilin sulphuric acid and Liebermann-Burchard I. The results of phytochemical analysis showed that Alpinia galanga n-hexane-extract contains alkaloids, flavonoids, saponins, triterpenoid, tannins and aromatic oil. Bioautogram revealed that there was one inhibition zone against Candida albicans. The active compounds in the inhibition zone were in Rf value 0.75 and 0.89. One out of the two compounds was identified as a compound from terpenoid group. Â Â Key Words: Compound, Extract, Alpinia galanga, Candida albicans

Antimicrobial and antioxidative activities of peptides from goat milk hydrolyzed with protease Kusumaningtyas, Eni; Widiastuti, Raphaella; Kusumaningrum, H. D.; Suhartono, M. T.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Milk is highly nutritious food containing protein as a good source of bioactive peptide that beneficial for health. This research was aimed to explore potency of bioactive peptide derived from goat milk as an antimicrobial and antioxidant. Milk was hydrolyzed by trypsin, chymotrypsin, pepsin, or protease Bacillus sp. E.13. The peptides obtained were screened for antimicrobial activities through incubation with Staphylococcus aureus, Listeria monocytogenes, Salmonella thyphimurium and Escherichia coli at 106 CFU/mL at 37Â°C for two hours and plated on Mueller Hinton agar. Antimicrobial activities were determined by comparing the total bacterial colonies to that of bacterial control without peptides addition. Oxidative activity was determined by 2.2â-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2.2-diphenyl-1-picrylhydrazyl (DPPH) assays. Antimicrobial activities were shown in peptides produced from hydrolysis of goat milk protein by pepsin at 37Â°C, pH 2 for 90 min and by Bacillus sp. E.13 protease at 55Â°C, pH 11 for 30 and 60 min but the activities were not detected in peptides from hydrolysis by trypsin and chymotrypsin. Peptide from protein hydrolysis by Bacillus sp. E.13 protease could inhibit Listeria monocytogenes, Salmonella thyphimurium and Escherichia coli up to 5 log cycles. The antimicrobial peptides could scavenge ABTS radical up to 86 % and DPPH radical up to 9 % at 68 Î¼g protein/mL. Results indicated that goat milk protein hydrolyzed by Bacillus sp. E.13 protease is potential as antimicrobes and antioxidant. Key Words: Goat Milk, Peptide, Antimicrobe, Antioxidant

Co-Authors Dwi Endrawati Estie Astuti H. D. Kusumaningrum, H. D. Lusi Sukmawati M. T. Suhartono Raphaella Widiastuti

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