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All Journal Jurnal Ilmiah Farmasi EKSAKTA: Journal of Sciences and Data Analysis JSFK (Jurnal Sains Farmasi & Klinis)
Sista Werdyani
Department Of Pharmacy, Universitas Islam Indonesia, Yogyakarta
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Determination of antioxidant active fraction from ethanol extract of benalu leaves (Scurrula atropurpurea (Bl.) Denser) which grows on rambutan trees Sista Werdyani; Denda Suli Hartati; Pinus Jumaryatno
Jurnal Ilmiah Farmasi Vol. 15 No. 2 (2019): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol15.iss2.art3

Abstract

IntisariLatar belakang: Aktivitas antioksidan benalu dapat berbeda apabila inang yang ditumbuhi berbeda. Penelusuran senyawa aktif antioksidan pada benalu rambutan belum pernah dilakukan, padahal pohon rambutan diketahui memiliki aktivitas antioksidan yang tinggi.Tujuan: Mengetahui golongan senyawa yang terkandung dalam fraksi aktif dengan aktivitas antioksidan tertinggi dan menghitung aktivitas antioksidannya menggunakan metode DPPH.Metode: Ekstraksi dilakukan dengan metode maserasi dan dilanjutkan dengan fraksinasi menggunakan kromatografi kolom vakum (KCV). Uji kualitatif kandungan senyawa ekstrak dan fraksi selanjutnya dilakukan dengan metode kromatografi lapis tipis (KLT) dengan bantuan reagen semprot. Aktivitas antioksidan ekstrak dan fraksi kemudian dihitung dengan metode DPPH.Hasil: Aktivitas antioksidan tertinggi adalah fraksi 5 dengan nilai IC50 7,211   ± 0,072. Kelompok senyawa pada fraksi 5 adalah flavonoid dan tannin. Kesimpulan: Senyawa aktif antioksidan dalam ekstrak etanol daun benalu rambutan terdapat dalam fraksi 5 dan diduga termasuk ke dalam golongan senyawa flavonoid dan atau tannin.  Kata kunci: Antioksidan, DPPH, benalu rambutan, Scurrula atropurpurea (Bl.) Denser Determination of Antioxidant Active Fraction from Ethanol Extract of Benalu Leaves (Scurrula atropurpurea (Bl.) Denser) which Grows on Rambutan TreesAbstractBackground: Antioxidant activity of benalu can be different if the overgrown host is different. The study for antioxidant active compounds in benalu which grows on rambutan trees has never been done, even though rambutan trees are known to have high antioxidant activity.Objective: to investigate the class of compounds contained in the active fraction with the highest antioxidant activity and to calculate their antioxidant activity using DPPH methods.Method: Extraction was carried out by maceration method and continued with fractionation using vacuum column chromatography (KCV). Qualitative tests of extract compound content and subsequent fractions were carried out by thin layer chromatography (TLC) method with the help of spray reagents. The antioxidant activity of extracts and fractions was then calculated using the DPPH method.Results: The highest antioxidant activity was fraction 5 with IC50 value 7.211 ± 0.072. The group of compounds in fraction 5 are flavonoids and tannins. Conclusion: The antioxidant active compounds in the ethanol extract of benalu which grows in rambutan were found in fraction 5 and were thought to belong to the class of flavonoids and or tannins. Keywords: antioxidant, DPPH, rambutan’s benalu, Scurrula atropurpurea (Bl.) Denser
ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT AND FRACTION OF SALAK FRUIT SEEDS ( Salacca zalacca (Gaertn.) Voss. ) USING DPPH (2,2-diphenyl-1-picrylhydrazyl) METHOD Sista Werdyani; Pinus Jumaryatno; Nur Khasanah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 17, ISSUE 2, August 2017
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol17.iss2.art5

Abstract

Salak seeds have been developed as a beverage, but there was still a little amount of research that focused on salak seeds. This research was conducted to find out the chemical compounds and the antioxidant activity of ethanolic extract and fraction of salak fruits seeds ( Salacca zalacca (Gaertn.) Voss. ) which have been grown extensively in Sleman Yogyakarta. Extraction was conducted using maceration, followed by fractionation using vacuum liquid chromatography. The identification of the chemical compounds contained in the ethanolic extract and fraction was performed by thin layer chromatography method, while the antioxidant activity was performed by DPPH method. Comparison of antioxidant activity was seen using IC50 values. The results showed that ethanol extract and fraction contained phenol, flavonoid, and tannin. The largest antioxidant activity was found in F7 with an IC50 value of 110.16 μg / ml.
In Vitro Tracing of Cytotoxic Compounds in Jarak Cina Stem Bark (Jatropha Multifida Linn.) Sista Werdyani; Annisa Fitria; Sari Rakhmawati
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 1, ISSUE 1, February 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol1.iss1.art2

Abstract

Cancer remains one of the diseases with increasing number of sufferers, but research on compounds that act as anti-cancer is also ongoing. Terpenoids have been known as a compound that can inhibit the proliferation of cancer cells. One of the medical plants that produce terpenoids is Jarak cina (Jatropha multifida Linn.). Therefore, the possibility of Jarak cina (Jatropha multifida Linn.) to have an cytotoxic activity on cancer cell proliferation is reasonably high. This study was conducted to determine the cytotoxic activity of Jarak cina (Jatropha multifida Linn.) bark extracts against cancer cell MCF-7. Jarak cina bark was extracted using the multilevel soxhlet extraction method with n-hexane, ethyl acetate, and ethanol as the solvents. All the three extracts were then tested against MCF-7 cancer cells using MTT (3-(4,5-dimethylthiazol-2-yl) - 2,5-diphenyltetrazolium bromide) method. Data analysis was performed for IC50 (ppm) parameter. The results showed that the IC50 of n-hexane extract was 313.21 ppm, while the ethyl acetate extract reached 258.38 ppm of IC50, and the IC50 of ethanol extract was 418.51 ppm. The highest potential of cytotoxicity was found in the ethyl acetate extract, so further testing would be required to optimize the proliferation inhibitory activity.
Aplikasi Gold Nanopartikel dengan Bahan Alam sebagai Kosmetik Pemutih Wajah: Tinjauan Sistematis Lisnawati Tiara Putri; Yandi Syukri; Sista Werdyani
Jurnal Sains Farmasi & Klinis Vol 8, No 2 (2021): J Sains Farm Klin 8(2), Agustus 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1068.426 KB) | DOI: 10.25077/jsfk.8.2.116-127.2021

Abstract

Penggunaan bahan alam sebagai pemutih memiliki keuntungan lebih aman meskipun penyerapan dalam kulit relatif rendah. Oleh karenanya, pengembangan nanopartikel emas (AuNPs) bahan alam dapat menjadi solusi permasalahan tersebut. Review ini bertujuan untuk mengumpulkan data terkait pengembangan bahan alam dengan nanopartikel emas yang berkhasiat sebagai agen pemutih. Identifikasi dilakukan dengan mencari literature melalui media pubmed, Science Direct, dan Google Scholar dengan menggunakan kata kunci ‘gold nanoparticle’, ’natural ingredients’, ’ cosmetics’, ‘tyrosinase inhibition’,’ melanin’ dan ’whitening’. Pencarian didasarkan pada kriteria inklusi dan eksklusi yang telah di tetapkan. Hasil evaluasi literatur menunjukkan pengembangan bahan alam sebagai pemutih kulit telah banyak dikembangkan, namun dikarenakan  sifat senyawa aktif sulit terserap menyebabkan hasil yang kurang maksimal dalam menghambat aktivitas tirosinase. Sehingga dilakukan modifikasi dalam bentuk nanopartikel emas untuk mempermudah proses penyerapan. Hasil pengujian aktivitas tirosinase bahan alam dalam bentuk nanopartikel emas lebih baik jika dibandingkan dengan bahan alam dalam bentuk ekstrak. Hal ini ditunjukan dengan nilai IC50 yang semakin kecil jika dibandingkan dengan dengan bahan alam dalam bentuk ekstrak
Co-Authors Annisa Fitria Denda Suli Hartati Lisnawati Tiara Putri Nur Khasanah Pinus Jumaryatno Sari Rakhmawati Yandi Syukri