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All Journal Jurnal Silvikultur Tropika (JST) JURNAL GALUNG TROPIKA Agrotech Journal
Muhammad Restu
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Social Sciences

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Informasi Genetik Bitti (Vitex cofassus) di Areal Sumber Daya Genetik (ASDG) BPTH Wilayah II Sulawesi Siti Halimah Larekeng; Zulfadilah Syam; M Musriati; Rathna Paelongan; Astuti Arif; Muhammad Restu; I Iswanto
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.801

Abstract

Bitti (Vitex cofassus) is one of the most important tree species in Sulawesi, which is used as material for phinisi boats. The aim study was to study the genetic diversity of Bitti in the genetic resources area in Bone Regency. This study used provenance Bulukumba, Muna, Kolaka, and North Luwu proof which was analyzed based on microsatellite markers. The resulting study showed evidence that provenance Muna has a Ho value 0f 0,65 or a relatively high genetic diversity value in comparison to provenance Bulukumba, Kolaka, and North Luwu.
POLLEN DISPERSAL PATTERNS EBONI LASITAE PROVENANCE BASED ON SIMPLE SEQUENCE REPEATS (SSR) Muhammad Restu; Gusmiaty .; Siti Halimah Larekeng
Jurnal Silvikultur Tropika Vol. 7 No. 3 (2016): Jurnal Silvikultur Tropika (Suplemen Desember)
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.7.3.S31-S35

Abstract

Parentage analysis has been used to evaluate pollen dispersal in ebony (Diosphyros celebica Bakh.). The objectives of this research were to evaluate (i) the dispersal of pollen, and (ii) the distance of pollen travel in ebony Lasitae provenances. The finding of this activities should be beneficial to support breeding of this ebony tree. There were 14 progeny arrays were harvested from 3 female parents. There were 32 ebony trees surrounding the female parents were analyses as the potential male parents for the progenies. Ebony tress were mapped according to their GPS position. All samples were genotyped using four SSR marker loci. Parentage analysis was done using CERVUS version 2.0 software. Results of the analysis indicated the evaluated markers were effective for assigning candidate male parents to all evaluated seedlings. There is no specific direction of donated pollen movement from assigned donor parents to the female ones. The donated pollens could come from assigned male parents in any directions relative to the female parent positions. Pollen dispersal pattern of ebony female parent occured in outcrossing pollination among its different donated trees. Based on the progenies analysis, ebony female parent is a dominantly outcrossing pollination species. Distances of pollen travel reach to 18 until 269 meter.Key words: Ebony, ssr marker, pollen dispersal.
Informasi Genetik Bitti (Vitex cofassus) di Areal Sumber Daya Genetik (ASDG) BPTH Wilayah II Sulawesi Siti Halimah Larekeng; Zulfadilah Syam; M Musriati; Rathna Paelongan; Astuti Arif; Muhammad Restu; I Iswanto
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.801

Abstract

Bitti (Vitex cofassus) is one of the most important tree species in Sulawesi, which is used as material for phinisi boats. The aim study was to study the genetic diversity of Bitti in the genetic resources area in Bone Regency. This study used provenance Bulukumba, Muna, Kolaka, and North Luwu proof which was analyzed based on microsatellite markers. The resulting study showed evidence that provenance Muna has a Ho value 0f 0,65 or a relatively high genetic diversity value in comparison to provenance Bulukumba, Kolaka, and North Luwu.
Genetic Diversity Hopea celebica an Indonesian endemic species by ISSR Marker Mirza Arsiaty Arsyad; Siti Halimah Larekeng; I. Iswanto; Muhammad Restu; Yuni Fitri Cahyaningsih; Michely Jauwdy Stevic
Agrotech Journal Vol 7, No 1 (2022): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v7i1.1737

Abstract

Indonesia is a mega-diversity country with numerous endemic plants distributed throughout its regions. An Indonesias’ island with the unique and the highest endemic plant species due to being located in the Wallace area is Sulawesi Island. Hopea celebica, an endemic species to Sulawesi Island, is currently categorized as endangered by IUCN. Here, we selected the ISSR primers suitable for the genetic study of H. celebica from Luwu and Konawe provenances and investigated their genetic diversity. Ten ISSR primers were employed in primer screening, and fifty H. celebicaindividuals were genetically analyzed for their genetic diversity. The selected ISSR primers for genetic diversity analysis were UBC 810, UBC 813, UBC 814, UBC 820, UBC 822, UBC 823, and UBC 827. The evaluated H. celebica individuals have high genetic diversity, and this information will be beneficial for designing H. celebica breeding and conservation strategies
Co-Authors Astuti Arif Gusmiaty . I Iswanto I. Iswanto M Musriati Michely Jauwdy Stevic Mirza Arsiaty Arsyad Rathna Paelongan SITI HALIMAH LAREKENG, SITI HALIMAH Yuni Fitri Cahyaningsih Zulfadilah Syam