Garuda - Garba Rujukan Digital

Toxocariasis in Animals and The Risks in Human Being Estuningsih, S Endah
Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Toxocariasis is usually caused by infection of Toxocara cati to cats, T. canis to dogs and T. vitulorum to cattle or buffaloes. The disease is not recognized and often underestimated by the owners or farmers. Toxocara is a pathogenic gastrointestinal worm and the larva could attack internal organs, cause diarrhea and kill the animals if the disease is not seriously handled . Some species of Toxocara is not only danger to the animals but it could also infect the human being . In human infected by with Toxocara, the larval can cause visceral larval migrans and resulting in symptom such as vomiting. Ocular larval migrans can also result from infection, which causes permanent eye damage in human. Prevention of toxocariasis could be done by avoiding contamination of the environment from eggs, coming from infected animals and also by treating with anthelmintic regularly. Key words: Toxocariasis, Toxocara cati. T. canis, T. vitulorum

The responses of eosinophil and packed cell volume (PCV) on sheep infected with Fasciola gigantica Widjajanti, S; Estuningsih, S Endah; Partoutomo, Subandriyo; Raadsma, H.W; Spithill, T.W; Piedrafita, D
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

The responses of eosinophil and packed cell volume (PCV) values were verified in infected sheep, in order to identify whether these parameters could be used to predict the flukes burden and their correlation with breed resistance. Fifteen Indonesian thin tail sheep (ET), 9 Merino sheep and 148 backcross sheep generated from mating of Merino sheep and F1 sheep (Merino X ET cross) were infected with 300 metacercariae of Fasciola gigantica. The blood samples were collected every 2 weeks by using EDTA venoject tubes in order to determine the amount of eosinophils and the PCV value. After 14 weeks of infection all of sheep were killed and the liver was collected in order to determine the number of flukes. The results showed that the amount of eosinophils increased 2 weeks after infection and reached the peak at week 4 after infection. The average of eosinophils in ET appeared higher than the other 2 breeds (Merino was the lowest and the backcross was in between). The correlation between the number of flukes recovered from the liver and the eosinophil counts were positive in ET and Merino, but negative in the backcross sheep. The PCV values remained constant along the trial, except at week 14 after infection; the PCV values were slightly decreased in backcross sheep and Merino sheep, but not in ET sheep. The correlation between number of flukes in the liver and the PCV values were negative in all breeds of sheep. These results suggested that the eosinophilic and PCVâs response of ET were higher compared to backcross and Merino sheep, thus that responses were thought to be associated with the resistant phenomenon. Â Key words: Fasciolosis, eosinophil, PCV, sheep

Comparison between antibody-elisa test and fecal egg count for detecting Fasciola gigantica infection in cattle Estuningsih, S Endah; Widjajanti, S; Adiwinata, Gatot
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

The comparison between antibody-ELISA test and fecal egg count for detection of natural infection of Fasciola gigantica in cattle was observed. One hundred and fifty samples of blood, feces and livers were collected from cattle slaughtered in the abattoir in Jakarta. Serum was collected from the blood samples and the level of antibody was determined by using antibody- ELISA test. The fecal samples were processed by using sedimentation technique in order to detect the present of F. Gigantica eggs. The livers were processed for liver flukes count. The result showed that from the liver examination, 38.7% cattle were negative flukes, 16% had 1-10 flukes, 34% cattle had 11-100 flukes and 11.3% cattle had more than 100 flukes. About 44.7% infected cattle had less than 100 eggs of F. gigantica per gram feces, however no eggs of F. gigantica were found in 13% infected cattle. The result of antibody-ELISA test showed that from 92 cattle infected with F. gigantica, 84 cattle had OD > 0.38 (range from 0.38-1.77) and 8 cattle had OD < 0.38 (range from 0.18-0.37). In contrast, from 58 cattle without flukes, 7 cattle had OD > 0.38 (range from 0.38-1.95) and 51 cattle with the OD < 0.38 (range from 0.1-0.33). The sensitivity of the fecal examination technique was 87% and the specificity was 100%. The sensitivity and specificity for antibody-ELISA test were 91 and 88% respectively. Â Key words: Antibody-ELISA, Fasciola gigantica, feces, liver

Characterisation of protein antigen from Fasciola gigantica of different age Estuningsih, S Endah; Widjajanti, S
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

The protein antigens extracted from adult fluke Fasciola gigantica, 3, 6 and 9 weeks old and newly excysted juvenile (NEJ) were identified using SDS-PAGE and immunoblotting techniques. Sera from fat-tailed sheep which artificially infected with the metacercariae of F. gigantica were used for immunoblotting. The results showed that the protein antigen profile of adult fluke, 6 and 9 weeks old flukes had similar. Bands with molecular weight between 24 kDa to 114 kDa. Protein bands with molecular weight <24 kDa and >198 kDa were also detected from the adult fluke. The use of immunoblotting technique, there were two antigenic protein molecules identified from adult fluke, NEJ and 3, 6, and 9 weeks old fluke with the molecular weight 46 kDa and 47 kDa. The protein band with molecular weight >198 kDa shown thicker on the NEJ than that of adult fluke, and 6 and 9 weeks old flukes. The role of protein with molecular weight of 46 and 47 kDa were the interested findings need to be evaluatedfor serological analysis. Â Key words : Protein antigen, Fasciola gigantica, SDS-PAGE, immunoblotting

Comparison between antibody-elisa test and fecal egg count for detecting Fasciola gigantica infection in cattle S Endah Estuningsih; S Widjajanti; Gatot Adiwinata
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The comparison between antibody-ELISA test and fecal egg count for detection of natural infection of Fasciola gigantica in cattle was observed. One hundred and fifty samples of blood, feces and livers were collected from cattle slaughtered in the abattoir in Jakarta. Serum was collected from the blood samples and the level of antibody was determined by using antibody- ELISA test. The fecal samples were processed by using sedimentation technique in order to detect the present of F. Gigantica eggs. The livers were processed for liver flukes count. The result showed that from the liver examination, 38.7% cattle were negative flukes, 16% had 1-10 flukes, 34% cattle had 11-100 flukes and 11.3% cattle had more than 100 flukes. About 44.7% infected cattle had less than 100 eggs of F. gigantica per gram feces, however no eggs of F. gigantica were found in 13% infected cattle. The result of antibody-ELISA test showed that from 92 cattle infected with F. gigantica, 84 cattle had OD > 0.38 (range from 0.38-1.77) and 8 cattle had OD < 0.38 (range from 0.18-0.37). In contrast, from 58 cattle without flukes, 7 cattle had OD > 0.38 (range from 0.38-1.95) and 51 cattle with the OD < 0.38 (range from 0.1-0.33). The sensitivity of the fecal examination technique was 87% and the specificity was 100%. The sensitivity and specificity for antibody-ELISA test were 91 and 88% respectively. Key words: Antibody-ELISA, Fasciola gigantica, feces, liver

Characterisation of protein antigen from Fasciola gigantica of different age S Endah Estuningsih; S Widjajanti
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The protein antigens extracted from adult fluke Fasciola gigantica, 3, 6 and 9 weeks old and newly excysted juvenile (NEJ) were identified using SDS-PAGE and immunoblotting techniques. Sera from fat-tailed sheep which artificially infected with the metacercariae of F. gigantica were used for immunoblotting. The results showed that the protein antigen profile of adult fluke, 6 and 9 weeks old flukes had similar. Bands with molecular weight between 24 kDa to 114 kDa. Protein bands with molecular weight <24 kDa and >198 kDa were also detected from the adult fluke. The use of immunoblotting technique, there were two antigenic protein molecules identified from adult fluke, NEJ and 3, 6, and 9 weeks old fluke with the molecular weight 46 kDa and 47 kDa. The protein band with molecular weight >198 kDa shown thicker on the NEJ than that of adult fluke, and 6 and 9 weeks old flukes. The role of protein with molecular weight of 46 and 47 kDa were the interested findings need to be evaluatedfor serological analysis. Key words : Protein antigen, Fasciola gigantica, SDS-PAGE, immunoblotting

The responses of eosinophil and packed cell volume (PCV) on sheep infected with Fasciola gigantica S Widjajanti; S Endah Estuningsih; Subandriyo Partoutomo; H.W Raadsma; T.W Spithill; D Piedrafita
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The responses of eosinophil and packed cell volume (PCV) values were verified in infected sheep, in order to identify whether these parameters could be used to predict the flukes burden and their correlation with breed resistance. Fifteen Indonesian thin tail sheep (ET), 9 Merino sheep and 148 backcross sheep generated from mating of Merino sheep and F1 sheep (Merino X ET cross) were infected with 300 metacercariae of Fasciola gigantica. The blood samples were collected every 2 weeks by using EDTA venoject tubes in order to determine the amount of eosinophils and the PCV value. After 14 weeks of infection all of sheep were killed and the liver was collected in order to determine the number of flukes. The results showed that the amount of eosinophils increased 2 weeks after infection and reached the peak at week 4 after infection. The average of eosinophils in ET appeared higher than the other 2 breeds (Merino was the lowest and the backcross was in between). The correlation between the number of flukes recovered from the liver and the eosinophil counts were positive in ET and Merino, but negative in the backcross sheep. The PCV values remained constant along the trial, except at week 14 after infection; the PCV values were slightly decreased in backcross sheep and Merino sheep, but not in ET sheep. The correlation between number of flukes in the liver and the PCV values were negative in all breeds of sheep. These results suggested that the eosinophilic and PCV’s response of ET were higher compared to backcross and Merino sheep, thus that responses were thought to be associated with the resistant phenomenon. Key words: Fasciolosis, eosinophil, PCV, sheep

Toxocariasis in Animals and The Risks in Human Being S Endah Estuningsih
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Toxocariasis is usually caused by infection of Toxocara cati to cats, T. canis to dogs and T. vitulorum to cattle or buffaloes. The disease is not recognized and often underestimated by the owners or farmers. Toxocara is a pathogenic gastrointestinal worm and the larva could attack internal organs, cause diarrhea and kill the animals if the disease is not seriously handled . Some species of Toxocara is not only danger to the animals but it could also infect the human being . In human infected by with Toxocara, the larval can cause visceral larval migrans and resulting in symptom such as vomiting. Ocular larval migrans can also result from infection, which causes permanent eye damage in human. Prevention of toxocariasis could be done by avoiding contamination of the environment from eggs, coming from infected animals and also by treating with anthelmintic regularly. Key words: Toxocariasis, Toxocara cati. T. canis, T. vitulorum

Title

Found 8 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 8 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search