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All Journal Current Biochemistry Journal of Science Innovare Journal Of Biology Education Research (JBER)
Dina Dyah Saputri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology

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Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer Dina Dyah Saputri; Maria Bintang; Fachriyan H Pasaribu
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.
PHYTOCHEMICAL SCREENING AND GASS CROMATOGRAPHY – MASS SPECTROMETER (GC-MS) ANALYSIS ETHANOL EXTRACT OF PURPLE SWEET POTATO (Ipomoea batatas L.) Surti Kurniasih; Dina Dyah Saputri
Journal of Science Innovare Vol 2, No 2 (2019): Journal of Science Innovare, September 2019
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.829 KB) | DOI: 10.33751/jsi.v2i2.1527

Abstract

Purple sweet potato (Ipomoea batatas L.) leaves are extremely versatile that possesses high value. Sweet potato (Ipomoea batatas L.) leaves have secondary metabolism compounds that used as antibacterial and antifungal. The content of secondary metabolite compounds contained in plants an important role in providing antibacterial and antifungal activity so that this study was conducted to develop the utilization of purple sweet potato leaves through phytochemical screening. Phytochemical screening aims to provide an overview of the class of compounds contained in purple sweet potato leaf plants including of alkaloids, steroids-triterpenoids, saponins, flavonoids, and tannins test. Samples were taken from the local community of Bogor. The extract is made by extracting the simplicia of positive purple sweet potato leaves containing alkaloid, steroids-triterpenoids, saponins, flavonoids, and tannins. Further identification of active compounds was performed by Gass Cromatography-Mass Spectrometer (GC-MS) analysis to detect the active compound contained in purple sweet potato leaf extract more specifically. GC-MS analysis results show several active compounds including: 1.4-Benzenediol (CAS) Hydroquinone, Benzenesulfonic acid 4-hydroxy (CAS), Hexadecanoic acid (CAS) Palmitic acid.
Analysis of Understory Vegetation in the Pusat Suaka Satwa Elang Jawa Area Aip Muhamad Irpan; Suci Siti Latifah; Dina Dyah Saputri; Muhamad Alfieansyah; Najla Nurul Amalia
Journal Of Biology Education Research (JBER) Vol. 6 No. 1 (2025): JBER (Journal Of Biology Education Research), Vol. 6 No. 1 (2025)
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55215/jber.v6i1.23

Abstract

Understory vegetation is the basic vegetation of the forest which can include grasses, shrubs, herbs and ferns which make an important contribution to ecological function and biodiversity. It contributes to nutrient cycling, soil stabilization, and forest regeneration. This study aims to analyze the structure of vegetation composition and diversity of understory plants in the Pusat Suaka Satwa Elang Jawa (PSSEJ). The research method uses square transects by determining the research location by purposive sampling. In each square transect, a plot measuring 2.5 m x 2.5 m was made. The number of plots made was 36 plots. The understory plants found were identified by cross-checking with the iNaturalist, PlantNet, Picture This - Plant identifier identification applications. Based on the results of the study, 52 species were found spread across 50 genera and 27 families. The dominant families are the Poaceae and Solanaceae families. The highest importance value index of Pseudechinolaena polystachya from the Poaceae family. The understory plant diversity index with a moderate category. It can be concluded that high species abundance does not always correlate with high INP values.
Co-Authors Aip Muhamad Irpan Fachriyan H Pasaribu MARIA BINTANG Muhamad Alfieansyah Najla Nurul Amalia Suci Siti Latifah Surti Kurniasih