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Potensi Liken Sebagai Bioindikator Kualitas Udara Di Kawasan Sentul Bogor Surti Kurniasih; Munarti Munarti; Dimas Prasaja; Anna Ayu Lestari
Jurnal Penelitian Ekosistem Dipterokarpa Vol 6, No 1 (2020): Jurnal Penelitian Ekosistem Dipterokarpa
Publisher : Jurnal Penelitian Ekosistem Dipterokarpa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jped.2020.6.1.17-24

Polusi udara merupakan salah satu masalah lingkungan yang terjadi di perkotaan terutama di daerah dengan kepadatan lalu lintas yang tinggi. Liken sangat peka terhadap parameter lingkungan seperti suhu, kelembaban, angin dan polusi udara sehingga dapat dijadikan sebagai bioindikator pencemaran udara. Penelitian ini bertujuan untuk mengetahui jenis liken yang dapat dijadikan sebagai bioindikator untuk memonitoring kualitas udara di Kawasan Sentul Eco Edu Tourism Forest. Metode penelitian yang digunakan adalah deskripif eksploratif, Pengambilan data liken dilakukan di tiga lokasi secara purposive sampling yaitu memilih secara sengaja lokasi yang dianggap memiliki tingkat polusi yang berbeda berdasarkan tingkat aktivitas manusia. Luas pengamatan liken dilakukan pada permukaan kulit batang pohon secara melingkar setinggi ±150 cm dari permukaan tanah, Analisis kandungan logam berat yaitu timbal (Pb) dan kromium (Cr) dengan metode Atomic Absorption Spectrophotometer (AAS). Hasil penelitian menunjukkan bahwa terdapat Sembilan jenis liken yang berhasil diidentifikasi dengan dua jenis tipe tallus yaitu Foliose dan Crustose. Jenis Parmelia paling sedikit ditemukan dan merupakan jenis liken yang sensitif sedangkan jenis liken Cryptochenia effusa ditemukan paling banyak dan terdapat pada semua lokasi pengamatan sehingga jenis ini tergolong toleran terhadap perubahan kualitas udara. Berdasarkan akumulasi Pb dan Cr pada tallus, jenis Parmelia lebih banyak mengakumulasi Pb dan Cr dibanding jenis Crypthocenia dan Physcia.

ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker Surti Kurniasih; Rubiyo Rubiyo; Asep Setiawan; Agus Purwantara; Sudarsono Sudarsono
Jurnal Penelitian Tanaman Industri Vol 17, No 4 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n4.2011.156-162

Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among evaluated cacao clones. Results of the experiment indicated that all SSR primer pairs evaluated were able to produce SSR markers for 29 cacao clones. The results also indicated that 34 out of 39 microsatellite loci evaluated were polymorphic, while 5 others were monomorphic. The total number of observed alleles among 29 clones was 132. Number of alleles per locus ranged from 4-8, with an average of 5.5 alelles per locus. Results of data analysis indicated that the PIC value was 0.665, the observed heterozigosity (Ho) was 0.651, and the gene diversity (He) was 0.720. The PIC, Ho, and He values were considered high. Genetic distances were evaluated using NTSys version 2.1 and dendrogram was constructed. Results of analysis indicated that 12 cacao clones evaluated were clustered in the first group with diversity coefficient of < 3.75. Nine cacao clones were in the second group but with the same value of diversity coefficient (<7.50). The rest of the cacao clones were in the third group with diversity coefficient of>7.50. Based on those finding, all SSR primer pairs evaluated could be used to analyze cacao genome and be useful for genetic diversity analysis of cacao germplasm. The SSR marker analysis in ICCRI cacao collections resulted in high PIC, high observed heterozygosity, and high genetic diversity.Key words: Theobroma cacao L, microsatelite, molecular marker, genetic diversity, heterozygosity AbstrakMarka mikrosatelit atau sekuens sederhana berulang (simple sequence repeat = SSR) terbukti merupakan alat yang bagus untuk identifikasi kultivar, analisis pedigree, dan evaluasi jarak genetik berbagai organisme. Penelitian ini bertujuan untuk:1) karakterisasi kakao koleksi Pusat penelitian Kopi dan Kakao Indonesia menggunakan marka SSR dan 2) analisis keragaman genetik klon-klon kakao koleksi dengan menggunakan marka SSR. Dalam penelitian ini, 39 pasangan primer SSR telah digunakan untuk amplifikasi DNA genomik dari 29 klon kakao. Skoring pita SSR hasil amplifikasi menggunakan masing-masing pasangan primer dilakukan secara terpisah dan digunakan untuk menentukan jarak genetik di antara klon kakao yang dievaluasi. Hasil percobaan menunjukkan bahwa semua pasangan primer SSR yang digunakan mampu menghasilkan pita DNA hasil amplifikasi (marka SSR) untuk 29 klon kakao yang diuji. Hasil penelitian juga menunjukkan bahwa 34 dari 39 lokus SSR yang dianalisis bersifat polimorfik sedangkan lima primer yang lain bersifat monomorfik. Dari 29 klon kakao yang dievaluasi, telah berhasil diamplifikasi sebanyak 132 alel, dengan kisaran antara 4-8 alel/lokus. Rataan jumlah alel per lokus sebanyak 5,50. Hasil analisis data yang dilakukan juga menunjukkan nilai PIC untuk marka SSR yang digunakan sebesar 0,665. Untuk populasi klon kakao yang dievaluasi, diperoleh nilai rataan heterosigositas pengamatan (Ho) sebesar 0,651 dan rataan diversitas gen (He) sebesar 0,720. Nilai PIC Ho dan He yang didapat tergolong tinggi. Berdasarkan analisis keragaman dengan menggunakan program NTSys, diperoleh hasil 12 klon kakao berada dalam grup pertama (koefisien keragaman<3,75) dan9 klon berada dalam grup kedua, dengan koefisien keragaman < 7,50. Sedangkan klon-klon lainnya mempunyai koefisien keragaman > 7,50. Berdasarkan hasil penelitian dan analisis data disimpulkan bahwa marka SSR dapat digunakan untuk menganalisis keragaman genetik plasma nutfah kakao. Tingkat polimorfisme yang dihasilkan marka SSR relatif tinggi. Tingkat heterosigositas plasma nutfah kakao koleksi Puslit Kopi dan Kakao Indonesiarelatif tinggi, dan keragaman genetiknyacukup tinggi.Kata kunci : Theobroma cacao L, mikrosatelit, marka molekuler, keragaman genetik, heterosigositas

ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker Surti Kurniasih; Rubiyo Rubiyo; Asep Setiawan; Agus Purwantara; Sudarsono Sudarsono
Jurnal Penelitian Tanaman Industri Vol 17, No 4 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n4.2011.156-162

Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among evaluated cacao clones. Results of the experiment indicated that all SSR primer pairs evaluated were able to produce SSR markers for 29 cacao clones. The results also indicated that 34 out of 39 microsatellite loci evaluated were polymorphic, while 5 others were monomorphic. The total number of observed alleles among 29 clones was 132. Number of alleles per locus ranged from 4-8, with an average of 5.5 alelles per locus. Results of data analysis indicated that the PIC value was 0.665, the observed heterozigosity (Ho) was 0.651, and the gene diversity (He) was 0.720. The PIC, Ho, and He values were considered high. Genetic distances were evaluated using NTSys version 2.1 and dendrogram was constructed. Results of analysis indicated that 12 cacao clones evaluated were clustered in the first group with diversity coefficient of < 3.75. Nine cacao clones were in the second group but with the same value of diversity coefficient (<7.50). The rest of the cacao clones were in the third group with diversity coefficient of>7.50. Based on those finding, all SSR primer pairs evaluated could be used to analyze cacao genome and be useful for genetic diversity analysis of cacao germplasm. The SSR marker analysis in ICCRI cacao collections resulted in high PIC, high observed heterozygosity, and high genetic diversity.Key words: Theobroma cacao L, microsatelite, molecular marker, genetic diversity, heterozygosity AbstrakMarka mikrosatelit atau sekuens sederhana berulang (simple sequence repeat = SSR) terbukti merupakan alat yang bagus untuk identifikasi kultivar, analisis pedigree, dan evaluasi jarak genetik berbagai organisme. Penelitian ini bertujuan untuk:1) karakterisasi kakao koleksi Pusat penelitian Kopi dan Kakao Indonesia menggunakan marka SSR dan 2) analisis keragaman genetik klon-klon kakao koleksi dengan menggunakan marka SSR. Dalam penelitian ini, 39 pasangan primer SSR telah digunakan untuk amplifikasi DNA genomik dari 29 klon kakao. Skoring pita SSR hasil amplifikasi menggunakan masing-masing pasangan primer dilakukan secara terpisah dan digunakan untuk menentukan jarak genetik di antara klon kakao yang dievaluasi. Hasil percobaan menunjukkan bahwa semua pasangan primer SSR yang digunakan mampu menghasilkan pita DNA hasil amplifikasi (marka SSR) untuk 29 klon kakao yang diuji. Hasil penelitian juga menunjukkan bahwa 34 dari 39 lokus SSR yang dianalisis bersifat polimorfik sedangkan lima primer yang lain bersifat monomorfik. Dari 29 klon kakao yang dievaluasi, telah berhasil diamplifikasi sebanyak 132 alel, dengan kisaran antara 4-8 alel/lokus. Rataan jumlah alel per lokus sebanyak 5,50. Hasil analisis data yang dilakukan juga menunjukkan nilai PIC untuk marka SSR yang digunakan sebesar 0,665. Untuk populasi klon kakao yang dievaluasi, diperoleh nilai rataan heterosigositas pengamatan (Ho) sebesar 0,651 dan rataan diversitas gen (He) sebesar 0,720. Nilai PIC Ho dan He yang didapat tergolong tinggi. Berdasarkan analisis keragaman dengan menggunakan program NTSys, diperoleh hasil 12 klon kakao berada dalam grup pertama (koefisien keragaman<3,75) dan9 klon berada dalam grup kedua, dengan koefisien keragaman < 7,50. Sedangkan klon-klon lainnya mempunyai koefisien keragaman > 7,50. Berdasarkan hasil penelitian dan analisis data disimpulkan bahwa marka SSR dapat digunakan untuk menganalisis keragaman genetik plasma nutfah kakao. Tingkat polimorfisme yang dihasilkan marka SSR relatif tinggi. Tingkat heterosigositas plasma nutfah kakao koleksi Puslit Kopi dan Kakao Indonesiarelatif tinggi, dan keragaman genetiknyacukup tinggi.Kata kunci : Theobroma cacao L, mikrosatelit, marka molekuler, keragaman genetik, heterosigositas

PHYTOCHEMICAL SCREENING AND GASS CROMATOGRAPHY – MASS SPECTROMETER (GC-MS) ANALYSIS ETHANOL EXTRACT OF PURPLE SWEET POTATO (Ipomoea batatas L.) Surti Kurniasih; Dina Dyah Saputri
Journal of Science Innovare Vol 2, No 2 (2019): Journal of Science Innovare, September 2019
Publisher : Universitas Pakuan

Purple sweet potato (Ipomoea batatas L.) leaves are extremely versatile that possesses high value. Sweet potato (Ipomoea batatas L.) leaves have secondary metabolism compounds that used as antibacterial and antifungal. The content of secondary metabolite compounds contained in plants an important role in providing antibacterial and antifungal activity so that this study was conducted to develop the utilization of purple sweet potato leaves through phytochemical screening. Phytochemical screening aims to provide an overview of the class of compounds contained in purple sweet potato leaf plants including of alkaloids, steroids-triterpenoids, saponins, flavonoids, and tannins test. Samples were taken from the local community of Bogor. The extract is made by extracting the simplicia of positive purple sweet potato leaves containing alkaloid, steroids-triterpenoids, saponins, flavonoids, and tannins. Further identification of active compounds was performed by Gass Cromatography-Mass Spectrometer (GC-MS) analysis to detect the active compound contained in purple sweet potato leaf extract more specifically. GC-MS analysis results show several active compounds including: 1.4-Benzenediol (CAS) Hydroquinone, Benzenesulfonic acid 4-hydroxy (CAS), Hexadecanoic acid (CAS) Palmitic acid.

EFEKTIVITAS KULIT BAWANG MERAH (Allium ascalonicum) TERHADAP PERTUMBUHAN PAKCOY (Brassica rapa) Yosi Dwi Ananda Putri; Surti Kurniasih; . Munarti
EKOLOGIA Vol 21, No 2 (2021): EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v21i2.3635

Mustard production in indonesia from 2017 to 2019 had been decreasing year by year, while market demanded for vegetable production had increased. On the other handed, shallot production in 2017 to 2019 increased, resulting in an abundance of shallot waste. Natural resources in the form of organic materials and organic waste that exist around farmers and the community in general could been made as organic fertilizer. Based on this, it was necessary to studied the effectiveness of liquid organic fertilizer (poc) of onion peel waste on the growth of pakcoy plants. The purpose of this studied was to studied the effect of onion peel waste on the growth of pakcoy. This studied used an experimental method with a completely randomized design (crd) consisting of 5 treatments and each treatment consisted of 5 replications, so the total number of experimental units was 25 experimental units. The concentration given to each treatment was p0 (0%), p1 ( 20%), p2 (40%), p3 (60%) and p4 (80%). Growth parameters observed was number of left, plant height and wet weight. The results showed that the administration of red onion peel waste had a significant effect on the growth of pakcoy on the three parameters observed. The optimal treatment for the number of left was p1(20%), plant height p3 (60%) and wet weight p1(20%) Keywords: Liquid Organic Fertilizer, Pakcoy, Shallots

KEANEKARAGAMAN JAMUR MAKROSKOPIS DI JALUR CURUG CIBEUREUM, TAMAN NASIONAL GUNUNG GEDE PANGRANGO Melly Audina Nurhikmawati; Surti Kurniasih; M. Taufik Awaludin
EKOLOGIA Vol 22, No 1 (2022): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4046

Gunung Gede Pangrango National Park (GGPNP) is a representative type of mountainous tropical rain forest ecosystem that is rich in biodiversity potential. Research on the diversity of Macrofungi has not been widely, especially in the Cibeureum Waterfall Trail, GGPNP. This study aims to study the diversity of Macrofungi in the Cibeureum Waterfall Trail, GGPNP route. The research was carried out from November 2020 to August 2021. Sampling was carried out by the roaming method. The results of the study of Macrofungi in the Cibeureum Waterfall Trail, GGPNP route obtained 2 phyllum, 9 orders, 22 families and 57 species with a total of 1,360 individuals. The value of the Shannon-Wiener diversity index (H') belongs to the medium category, the evenness index (E) is moderate and the dominance index (D) is low. The abiotic conditions in this area based on the recording of temperature, pH, humidity and light intensity indicate conditions that support the life of Macrofungi in the Cibeureum Waterfall Trail, GGPNP route.

Potensi Liken Sebagai Bioindikator Kualitas Udara Di Kawasan Sentul Bogor Surti Kurniasih; Munarti Munarti; Dimas Prasaja; Anna Ayu Lestari
Jurnal Penelitian Ekosistem Dipterokarpa Vol 6, No 1 (2020): Jurnal Penelitian Ekosistem Dipterokarpa
Publisher : Jurnal Penelitian Ekosistem Dipterokarpa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jped.2020.6.1.17-24

Polusi udara merupakan salah satu masalah lingkungan yang terjadi di perkotaan terutama di daerah dengan kepadatan lalu lintas yang tinggi. Liken sangat peka terhadap parameter lingkungan seperti suhu, kelembaban, angin dan polusi udara sehingga dapat dijadikan sebagai bioindikator pencemaran udara. Penelitian ini bertujuan untuk mengetahui jenis liken yang dapat dijadikan sebagai bioindikator untuk memonitoring kualitas udara di Kawasan Sentul Eco Edu Tourism Forest. Metode penelitian yang digunakan adalah deskripif eksploratif, Pengambilan data liken dilakukan di tiga lokasi secara purposive sampling yaitu memilih secara sengaja lokasi yang dianggap memiliki tingkat polusi yang berbeda berdasarkan tingkat aktivitas manusia. Luas pengamatan liken dilakukan pada permukaan kulit batang pohon secara melingkar setinggi ±150 cm dari permukaan tanah, Analisis kandungan logam berat yaitu timbal (Pb) dan kromium (Cr) dengan metode Atomic Absorption Spectrophotometer (AAS). Hasil penelitian menunjukkan bahwa terdapat Sembilan jenis liken yang berhasil diidentifikasi dengan dua jenis tipe tallus yaitu Foliose dan Crustose. Jenis Parmelia paling sedikit ditemukan dan merupakan jenis liken yang sensitif sedangkan jenis liken Cryptochenia effusa ditemukan paling banyak dan terdapat pada semua lokasi pengamatan sehingga jenis ini tergolong toleran terhadap perubahan kualitas udara. Berdasarkan akumulasi Pb dan Cr pada tallus, jenis Parmelia lebih banyak mengakumulasi Pb dan Cr dibanding jenis Crypthocenia dan Physcia.

Enhancing Students’ Creative Thinking Skills through Biotechnology Module based Socio-Scientific Issues Indarini Dwi Pursitasari; Nia Alfitriyani; Surti Kurniasih
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 2 (2022): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i2.1438

This study aims to enhance students’ creative thinking skills through the biotechnology module based on socio-scientific issues. This is pre-experimental research with a one-group pretest-posttest design. The learning is carried out online using the Zoom meeting application, which is familiar to the students. The instrument for collecting data is a valid creative thinking skill test sheet. The students’ responses were analyzed using a questionnaire. The data analysis was done by descriptive quantitative and inferential. The result of the inferential statistical test shows that there is a significant effect of using SSI-based biotechnology modules on students’ creative thinking skills. The SSI-based biotechnology module is effective to improve creative thinking skills, indicated by the N-Gain value is 0.63 in the moderate category. The learning implementation has an average value above 75%, indicating that the learning process is carried out very well. 87.50% of the students gave excellent responses to the use of the SSI-based module in learning. There is an increase in the N-Gain value in creative thinking skills, so we can conclude that the biotechnology module based on the socio-scientific issue is effective to improve creative thinking skills

Assessment for Learning: Changes in the Role of Assessment in Learning Tatang Mulyana; Surti Kurniasih; Didit Ardianto
IJORER : International Journal of Recent Educational Research Vol. 2 No. 5 (2021): September
Publisher : Faculty of Teacher Training and Education Muhammadiyah University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46245/ijorer.v2i5.146

The purpose of this research is to describe the state of assessment for learning (AfL) in science learning. The research was conducted in February 2021. This research was conducted with a descriptive method using data collection techniques, namely a survey via google form. Before the instrument is used, calibration and testing are carried out and the test results of all items are declared valid and reliable. The results showed that the teacher agreed to the use of Assessment for Learning (AfL) in science learning, which could activate the learning process and accelerate the achievement of goals. In addition to the use of Assessment for Learning (AfL) in science learning, teachers also answered effectively in developing students' literacy skills according to subject coverage. Assesment for Learning (AfL) provides an overview of how students learn and achieve learning goals effectively that can be reported to students' parents. The obstacle in implementing assessment for learning (AfL) is that teachers still find it difficult to compile and develop assessment-based learning designs that are in accordance with process standards, so that most of these teachers adopt learning designs that are already available from various sources, so that the role of assessment is not yet a an integral part of the teaching and learning process.

STUDENT TEACHING MATERIALS AND SCIENCE LITERATURE IN SCIENCE LEARNING DURING THE COVID-19 PANDEMIC Eka Puspita Rahayu; Indarini Dwi Pursitasari; Surti Kurniasih
JPPS (Jurnal Penelitian Pendidikan Sains) Vol. 11 No. 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jpps.v11n1.p52-61

The research aims to analyze the teaching materials needed by the teacher and the level of students' scientific literacy in the science learning process in the COVID-19 pandemic era. The descriptive quantitative method was utilized in the present study. The subject of the survey is 35 science teachers in Bogor and 150 grade VIII students at one of the junior high schools in the Bogor district. The instruments used in this study were questionnaires and scientific literacy tests. The collected data were analyzed quantitatively by calculating the percentage acquisition of students' scientific literacy then categorized. The study results revealed that the teaching materials used were not proportional in containing aspects of scientific literacy; 50% of students have scientific literacy ability in the low category, 40% in the moderate category, and 10% in the high category. The questionnaire results indicate that the factors affecting students' scientific literacy are their interest in science, learning motivation, teacher strategies in learning, and the facilities.

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