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Ratna Damayanti Sianturi
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REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture) Ratna Damayanti Sianturi; Supriyanto Supriyanto; Arum Sekar Wulandari; Benny Subandy
Jurnal Penelitian Hutan Tanaman Vol 14, No 1 (2017): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2838.211 KB) | DOI: 10.20886/jpht.2017.14.1.1-17

Abstract

 ABSTRACTembesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program,and it can be achieved by tissue culture. The objective of the research was to find out the best modificationmedium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitiousshoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitiousshoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The resultsshowed (1) addition of BAP 1.5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves;(2) addition of BAP 0.1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3).clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant inacclimatization stage.Keywords : Fagraea fragrans, tissue culture, medium, organogenesisABSTRAKTembesu (Fagraea fragrans Roxb.) merupakan jenis pohon yang mampu tumbuh di hutan rawa gambut dan bernilai ekonomi tinggi. Untuk mendukung program rehabilitasi lahan gambut, maka diperlukan bibit yangberkualitas, jumlah yang cukup dan tepat waktu. Upaya yang diperlukan dalam menyediakan bibit tersebutadalah perbanyakan bibit melalui teknik kultur jaringan. Tujuan penelitian adalah mendapatkan komposisimedia tumbuh MS dan zat pengatur tumbuh yang tepat dalam pembentukan dan perbanyakan tunas adventifdari daun tembesu. Metode penelitian terdiri dari persiapan bahan tanaman, induksi tunas adventif,perbanyakan tunas adventif, elongasi, pengakaran dan aklimatisasi. Penelitian menunjukkan bahwa hasilyang terbaik yaitu (1) untuk menginduksi tunas adventif diperlukan media MS (80 mmol N) denganpenambahan BAP 1,5 ppm; (2) untuk perbanyakan tunas adventif diperlukan media MS (60 mmol N) denganpenambahan 0,1 ppm BAP; (3) pada tahap elongasi dan pengakaran lebih baik menggunakan tunas adventifdari klon 2; dan (4) pada tahap aklimatisasi lebih baik menggunakan tunas adventif klon 4.Kata kunci : Fagraea fragrans, kultur jaringan, media, organogenesis 
Correlation of Morphological Characteristics of Nyamplung Seedling (Calophyllum inophyllum L) with The Performance at Field Condition Ratna Damayanti Sianturi; Dede J Sudrajat
Jurnal Wasian Vol 6, No 1 (2019): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jwas.v6i1.2998

Abstract

The aim of this research is to assess the correlation of morphological characteristics of nyamplung seedling (Calophyllum inophyllum L) on field growth of two years age after outplanting in Parungpanjang, Bogor. Three seedlots collected from Carita, Pangandaran and Purworejo were grown in seedbeds with different ages (four months and six months). The seedlings are mixed and grouped according to their height and root collar diameter. Each group of seedlings is divided into 5 height classes and each class is divided into two diameter classes, so there are 10 classes of seed morphology. The parameters of seedling were observed on sturdirness quotient, dry weight, root length, shoot-root ratio, and number of leaves. The seedlings were planted in randomized completely block design (three seeds origins, 10 morphological class, three blocks, 30 seedlings per block). Seedling survival, height, and root collar diameter growth were assessed on two years after outplanting. The result showed that morphological classification affected on seedling survival, height and root collar diameter growth. Seedling height and root collar diameter were significantly correlated with other parameters and also with field growth. Two-year-old Nyamplung on field can grow significantly if we use seedling with a height above 31 cm and a diameter more than 5.1 mm. In addition, the ratio of height and diameter and seed quality index are become important consideration in seed selection.Keywords: Calophyllum inophyllum, diameter, height, morphology, seedling quality
Correlation of Morphological Characteristics of Nyamplung Seedling (Calophyllum inophyllum L) with The Performance at Field Condition Ratna Damayanti Sianturi; Dede J Sudrajat
Jurnal Wasian Vol 6, No 1 (2019): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1328.829 KB) | DOI: 10.20886/jwas.v6i1.2998

Abstract

The aim of this research is to assess the correlation of morphological characteristics of nyamplung seedling (Calophyllum inophyllum L) on field growth of two years age after outplanting in Parungpanjang, Bogor. Three seedlots collected from Carita, Pangandaran and Purworejo were grown in seedbeds with different ages (four months and six months). The seedlings are mixed and grouped according to their height and root collar diameter. Each group of seedlings is divided into 5 height classes and each class is divided into two diameter classes, so there are 10 classes of seed morphology. The parameters of seedling were observed on sturdirness quotient, dry weight, root length, shoot-root ratio, and number of leaves. The seedlings were planted in randomized completely block design (three seeds origins, 10 morphological class, three blocks, 30 seedlings per block). Seedling survival, height, and root collar diameter growth were assessed on two years after outplanting. The result showed that morphological classification affected on seedling survival, height and root collar diameter growth. Seedling height and root collar diameter were significantly correlated with other parameters and also with field growth. Two-year-old Nyamplung on field can grow significantly if we use seedling with a height above 31 cm and a diameter more than 5.1 mm. In addition, the ratio of height and diameter and seed quality index are become important consideration in seed selection.Keywords: Calophyllum inophyllum, diameter, height, morphology, seedling quality
Co-Authors Arum Sekar Wulandari Benny Subandy Dede J Sudrajat Supriyanto Supriyanto