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Identifikasi Parasit Helmint Pada Ternak Sapi Di Dukuh Gading Wetan Klaten Dengan Metode Sedimentasi Fitria Diniah Janah Sayekti; Dwi Haryatmi
Cendekia Journal of Pharmacy Vol 3, No 1 (2019): Cendekia Journal of Pharmacy
Publisher : STIKES Cendekia Utama Kudus

Parasit helmint bersifat zoonosis yang dapat menginfeksi manusia dan menyebabkan hepatalgia, demam, dan penurunan berat badan. Kondisi peternakan di Gading Wetan Kecamatan Tulung Klaten yang masih tradisional dan dekat dengan pemukiman warga memungkinkan terjadinya penularan infeksi parasit helmint. Tujuan penelitian ini adalah untuk mengetahui infeksi parasit helmint pada ternak sapi di Dukuh Gading Wetan Kecamatan Tulung, Klaten. Pemeriksaan parasit helmint menggunakan feses sapi dengan metode sedimentasi. Berdasarkan hasil penelitian, menunjukkan prevalensi parasit helmint pada sapi di ternak di Dukuh Gading Wetan, Kecamatan Tulung, Klaten sebesar 50 % yaitu Fasciola sp. sebesar 25%, Trichuris sp. 10%, Hook worm 10%, telur tidak terindentifikasi 20%.

AKTIVITAS ANTIANGIOGENESIS EKSTRAK BERAS HITAM (Oryza sativa L. indica) PADA CHORIOALANTOIC MEMBRANE (CAM) SEBAGAI KANDIDAT ANTIKANKER ANTIANGIOGENESIS ACTIVITY OF BLACK RICE (Oryza sativa L. indica) ON CHORIOALANTOIC MEMBRANE (CAM) AS ANTICANCER CANDIDATE Fitria Diniah Janah Sayekti; Muhammad Taufiq Qurrohman
Indonesian Journal of Applied Sciences Vol 8, No 1 (2018)
Publisher : Universitas Padjadjaran

AbstrakAngiogenesis memberikan kontribusi pada karsinogenesis atau pertumbuhan sel kanker yang tidak terkendali. Pertumbuhan tumor atau kanker dapat dikontrol melalui penghambatan angiogenesis. Salah satu bahan alam yang berpotensi sebagai bahan pangan fungsional prevensi kanker adalah beras hitam. Penelitian ini bertujuan untuk mempelajari dan mengetahui aktivitas antiangiogenesis ekstrak beras hitam (Oryza sativa L. indica) pada Chorioallantoic Membrane (CAM) sebagai kandidat antikanker. Parameter yang diamati dalam penelitian adalah banyaknya pembuluh darah baru atau respon angiogenesis pada CAM setelah pemberian ekstrak beras hitam dengan konsentrasi masing-masing 20, 25, 50, 60 dan 75 µg/mL. Ekstrak beras hitam menghambat pembentukan pembuluh darah pada CAM. Rata-rata jumlah pembuluh darah pada konsentrasi ekstrak beras hitam 20, 25, 50, 60 dan 75 µg/mL berturut-turut adalah 27,6; 21,0; 3,67; 3,0; 2,0. Pada kontrol dengan larutan NaCl 0,9 %, jumlah rata-rata pembuluh darah adalah adalah 35,33. Kata Kunci : Beras hitam (Oryza sativa L. indica), angiogenesis, antikanker AbstractAngiogenesis contributes to uncontrolled carcinogenesis or cancer cell growth. Tumor or cancer growth can be controlled through inhibition of angiogenesis. One of the natural ingredients that have the potential as a cancer prevention functional food ingredient is black rice. This study aims to study and determine the antiangiogenesis activity of black rice extract (Oryza sativa L. indica) on the Chorioallantoic Membrane (CAM) as an anticancer candidate. The parameters observed in the study were the number of new blood vessels or the response of angiogenesis to CAM after the administration of black rice extract with a concentration of 20, 25, 50, 60 and 75 µg / mL, respectively. The higher the concentration of black rice extract showed a decrease in the number of blood vessels formed. The average number of blood vessels at concentrations of black rice extract 20, 25, 50, 60 and 75 µg / mL were 27.6; 21.0; 3.67; 3.0; 2.0. In the control with 0.9% NaCl solution, the average number of blood vessels was 35.33. Keywords: Black rice ( Oryza sativa L. indica), angiogenesis, anticancer

Pengaruh Kombinasi Buah Jeruk Nipis dan Buah Mengkudu Terhadap Mortalitas Pediculus humanus capitis Fitria Diniah Janah Sayekti; Muhammad Taufiq Qurrohman; Damar Ajeng Priyandari; Chris Srikandini
At-Taqaddum Vol 12, No 1 (2020)
Publisher : Quality Assurance Institute (LPM) State Islamic University Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/at.v12i1.5148

Head lice (Pediculus humanus capitis) greatly interferes with human activity because it can cause itching of the head, redness and severe infections. The use of natural insecticides is recommended, because natural insecticides are considered safer. Potential anti lice plants are lime and noni. The combination of the two is believed to have a more effective effect on head lice mortality. This study aims to study and determine the effect of a combination of lime juice and noni on the mortality of Pediculus humanus capitis. The concentration of lime and noni which are used are 25%, 50%, 75% and 100%, respectively. The combination of test materials used was 25% limes 75% noni; 50% lime 50% noni; 75% lime and 25% noni, negative control and positive control. The combination of the extract of noni and lime juice had the highest mortality effect on Pediculus humanus capitis with a concentration of 25% combination of lime; 75% noni.

Combination of Corn, Cassava and Yellow Sweet Potato as Media Growth of Candida Albicans Muhammad Taufiq Qurrahman; Fitria Diniah Janah Sayekti; Dwi Haryatmi
Indonesian Journal of Global Health Research Vol 4 No 3 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Fungal infections are quite common in people in tropical countries including Indonesia. Candida albicans is a fungus that can cause infection in humans. Infection caused by Candida albicans is called candidiasis. Diagnosis of Candida albicans with a culture system aims to identify and confirm the results of microscopic examination. Alternative media for fungal growth from various carbohydrate sources have been widely used. Alternative media for fungal growth used included corn, cassava and sweet potato. This study aims to determine the growth of Candida albicans on media with a combination of corn, cassava and sweet potato. The source of data was obtained from primary data, namely data on the results of differences in growth observed from the number of Candida albicans colonies formed on a combination of corn, cassava, and yellow sweet potato media. Data analysis was carried out by statistical tests, including normality test and homogeneity test. If the normality test and homogeneity test meet the requirements, then proceed with the ANOVA and post hoc tests. The results showed that the growth of Candida albicans after 48 hours of incubation, the average number of colonies on corn-cassava media was 58.4x1011 CFU/ml colonies with a colony diameter of 1 mm. Colonies on yellow sweet potato-corn media were 51.5x1011 CFU/ml with a diameter of 1 mm. Colonies on PDA media were 24.8x1011 CFU/ml with a diameter of 1-2mm. In this study, it can be seen that there is a significant difference (p<0.05) in the growth of Candida albicans fungal colonies in each group of media with the highest colony growth, namely on corn and cassava media.

Combination of Corn, Cassava and Yellow Sweet Potato as Media Growth of Candida Albicans Muhammad Taufiq Qurrahman; Fitria Diniah Janah Sayekti; Dwi Haryatmi
Indonesian Journal of Global Health Research Vol 4 No 3 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Fungal infections are quite common in people in tropical countries including Indonesia. Candida albicans is a fungus that can cause infection in humans. Infection caused by Candida albicans is called candidiasis. Diagnosis of Candida albicans with a culture system aims to identify and confirm the results of microscopic examination. Alternative media for fungal growth from various carbohydrate sources have been widely used. Alternative media for fungal growth used included corn, cassava and sweet potato. This study aims to determine the growth of Candida albicans on media with a combination of corn, cassava and sweet potato. The source of data was obtained from primary data, namely data on the results of differences in growth observed from the number of Candida albicans colonies formed on a combination of corn, cassava, and yellow sweet potato media. Data analysis was carried out by statistical tests, including normality test and homogeneity test. If the normality test and homogeneity test meet the requirements, then proceed with the ANOVA and post hoc tests. The results showed that the growth of Candida albicans after 48 hours of incubation, the average number of colonies on corn-cassava media was 58.4x1011 CFU/ml colonies with a colony diameter of 1 mm. Colonies on yellow sweet potato-corn media were 51.5x1011 CFU/ml with a diameter of 1 mm. Colonies on PDA media were 24.8x1011 CFU/ml with a diameter of 1-2mm. In this study, it can be seen that there is a significant difference (p<0.05) in the growth of Candida albicans fungal colonies in each group of media with the highest colony growth, namely on corn and cassava media.

PREVALENSI INFEKSI SOIL TRANSMITTED HELMINTH PADA PENGRAJIN GENTENG DI DESA WIRUN MOJOLABAN SUKOHARJO MENGGUNAKAN METODE DIRECT DAN INDIRECT Fitria Diniah Janah Sayekti; Muhammad Taufiq Qurrohman; Dwi Haryami
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2428

Background : Soil Transmitted Helminths (STH) is an intestinal nematode which in its life cycle requires soil for the egg maturation process. Included in this group are Ascaris lumbricoides, Trichuris trichiura, and Hookworm. Lack of attention to clean living behavior can make a person easily infected with helminthiasis. One profession that is closely related to STH infection is work related to soil, including tile craftsmen.Wirun Village in Mojolaban District, Sukoharjo Regency has residents who partly work as roof tile craftsmen. In carrying out their profession, tile craftsmen sometimes do not use personal protective equipment such as gloves and footwear so that they are in direct contact with the ground and have a potential risk of STH infection. Aims:This study aims to determine the prevalence of Soil Transmitted Helminths (STH) using two methods, direct and indirect.Methode :The direct method is carried out to see the presence of eggs or STH larvae with a faster time. The indirect method is carried out by adding special ingredients that can catch worm eggs or larvae. The indirect technique used in this study was to use saturated NaCl flotation. The results:Based on the research results, it can be seen that 6.67% of the respondents were positively infected with STH using either direct or indirect methods. The STH species found was Ascaris lumbricoides. Based on this, it can be seen that the use of the 2 methods does not affect the different results. Conclusion : The prevalence of soil-transmitted helminth infection among tile craftsmen in Wirun village is low because only 6.67% of respondents were found to be positively infected

Description of polimorfism FTO Gene (Fat and Mass Obesity associated) rs9939609 on Obesity and Non Obesity Subject Fitria Diniah Janah Sayekti; Alfian Marfianto
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.16547

Obesity is a major risk factor for several chronic diseases and is a major health and economic burden on society. The FTO gene (Fat and Mass Obesity associated) was identified as a gene that has an influence on the incidence of obesity according to Genome-wide Association Studies. Examination of the FTO gene polymorphism rs9939609 using 15 samples of obese subjects and 15 samples of non-obese subjects at Prodia Pusat Jakarta employees. This study used descriptive research to describe the polymorphism or variation of the FTO (Fat mass and obesity-associated) gene rs9939609 in obese and non-obese objects among Prodia Pusat Jakarta employees. In this study, non-obese subjects obtained 100% AT allele results. This indicates that genetically there is a potential risk factor for obesity. In obese subjects, 60% of the TT alleles were found. Genetically it should not be a risk allele for the risk of obesity. This can be caused by lifestyle or diet. In this study, an AA allele of 6.67% was found, indicating a greater risk of obesity. From the study, it was concluded that the percentage of the FTO gene polymorphism rs9939609 in obese and non-obese subjects in Prodia Pusat Jakarta employees was the AT allele 100% for obese subjects and for obese subjects. non-obese subject of 33.33%. The AA allele is 6.67% for obese subjects, and the TT allele is 60% for obese subjects.

EXAMINATION RESULTS OF TCM AND RISK FACTORS FOR PULMONARY TUBERCULOSIS IN HIV PATIENTS Fitria Diniah Janah Sayekti; Indah Rismarwati
Journal of Nursing and Health Vol. 9 No. 2 (2024): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v9i2.363

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