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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Asri Insiana Putri
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan
Agriculture, Biological Sciences & Forestry

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Ponngamia pinnata- Rhi zobium: SPESIES SIMBIOTIK BERNILAI KONSEVASI TINGGI Asri Insiana Putri
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 2 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.2.73-81

Abstract

Pongamia pinnata is an arboreal legume with high conservation value (Conference of The Parties to The Convention on Biological Diversity at the IX/2 nd  on plants of high conservation volue criteria). Biotechnology breeding program with the selection  of legume crops that have a high nitrogen use efficiency becomes an important issue to improve productivity and quality of P. pinnata.Thus the important symbiotic effectiveness test done to improve the ability of N fixation as the main element forming a plant tissues . The purpose of this study was to obtain Rhizobium strain from different P. pinannta habitats which has the highest effectiveness compared with strain Rhizobium sp. Commercial (USDA 122) and controls to P. pinnata seedlings. The parameters used are the number of nodules, plant height, root length and diameter . From this activity obtained 3 strains of Ambon and 2 strains of Banyuwangi .  Strains selected on the basis of colony growth speed of Rhizobium sp.in vitro.The average number of nodules, plant height,  root length and diameter of P. pinnata that are inoculated with strain  AMI. AM2, AM3, Bal and Ba2 from seram, Ambon higher than from Baluran , Banyuwangi. On average, the highest number of nodules occurred in simbiose of P.  pinnata) Ambon  Rhizobium AM3  (50 ± 0,66) eguels P. pinnata Ambon –Rhizobium USDA 122 (50 ± 0,88); the best plant height occurred in P. pinnata Ambon-Rhizobium Ba2 (72cm ± 1,14) ; the best root length  (25 cm ± 0.33) and the best stem diameter (4.0 mm  ±  0.33) occurred in P. pinnata Ambon-Rhizobium AM2. The highest symbiose effectiveness value of P. pinnata-Rhizobium occureed in P. pinnata origin from Ambon with Rhizobium strain AM3 (32,45).
Inisiasi Tunas Acacia Hibrid (Acacia mangium x A.Auriculiformis) Secara In Vitro Sri Sunarti; Asri Insiana Putri
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 1 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.1.11-22

Abstract

Preliminary shoot groeth on acacia hybrids (Acacia mangium x A. Auriculiformis) was observed to assess their in- vitro shoot initiation. Explants were taken from 4 months old seedlings of A. Mangium, A. Auriculiformis and their hybrid. Murashige & Skoog’s (MS) medium was used supplemented with various concentration of BAP, IBA,NAA, and GA4. Parameters measured were bud breaking, number average number of leaves of acacia hybrid was 7 and 1.9 cm and 5.2 respectively, found in medium Ak2 culture. Bud breaking of acacia hybrids on that medium happened 15 days earlier than pure Acacia. This finding showed that acacia hybrid could be propagated by  micro propagation succesfully.
Kajian Glycocalyx bakteri Pada Kontaminasi Ulin (Eusideroxylon zwageri) In-Vitro Asri Insiana Putri
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 1 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1179.135 KB) | DOI: 10.20886/jpth.2009.3.1.33-42

Abstract

In-Vitro contamination of plant tissue cultures by bacteria is one of the most serious problem in plant micropropagation. Ulin (Eusideroxylon zwageri) bacterial contamination in vitro may survive surface sterization of the explant because they are in interior tissue and some Bacterial spores can also survive the sterilization procedure even if they are un the tissue surface. Bacteria  secrete some sort of glycocalyx, an auter viscous covering of fibers extending from the Bacterium. This Study was conducted to examine the colony thickness and to observe the bacteria glycocalyx contaminant of ulin in vitro folowing three surface explant sterization treatments with fungisida, detergent, alkhohol,HgCl2  and Na2CLO3 as antimicrobial agents. The longer the sterilization time the lesser the bacterial colony thickness farmed. Transmission binocular microscopy showed that distribution of glycocalyx material surrounding coccus ulin bacterial contaminant cells were capsulated like Steptococcus lactis or Enterobacter aerogenes. 
Co-Authors Sri Sunarti